Functional divergence of the Arg/N-degron pathway between the crop Brassica rapa and the model plant Arabidopsis thaliana https://www.biorxiv.org/content/10.64898/2025.12.04.692320v1
December 9, 2025 at 6:02 AM
Functional divergence of the Arg/N-degron pathway between the crop Brassica rapa and the model plant Arabidopsis thaliana https://www.biorxiv.org/content/10.64898/2025.12.04.692320v1
Functional divergence of the Arg/N-degron pathway between the crop Brassica rapa and the model plant Arabidopsis thaliana https://www.biorxiv.org/content/10.64898/2025.12.04.692320v1
December 9, 2025 at 6:02 AM
Functional divergence of the Arg/N-degron pathway between the crop Brassica rapa and the model plant Arabidopsis thaliana https://www.biorxiv.org/content/10.64898/2025.12.04.692320v1
Our latest publication
Physiological and transcriptomic dissection of submergence tolerance in cabbage highlights ERF-VII and N-degron regulation
doi.org/10.1016/j.en...
Physiological and transcriptomic dissection of submergence tolerance in cabbage highlights ERF-VII and N-degron regulation
doi.org/10.1016/j.en...
Redirecting
doi.org
December 2, 2025 at 5:35 AM
Our latest publication
Physiological and transcriptomic dissection of submergence tolerance in cabbage highlights ERF-VII and N-degron regulation
doi.org/10.1016/j.en...
Physiological and transcriptomic dissection of submergence tolerance in cabbage highlights ERF-VII and N-degron regulation
doi.org/10.1016/j.en...
Does anybody know if an N-degron mechanism has been discovered for proteins with unblocked N-terminal alanines? I can't seem to find one, but finding anything in "the literature" has become a hit or miss proposition.
December 1, 2025 at 5:13 PM
Does anybody know if an N-degron mechanism has been discovered for proteins with unblocked N-terminal alanines? I can't seem to find one, but finding anything in "the literature" has become a hit or miss proposition.
Does anybody know if an N-degron mechanism has been discovered for proteins with unblocked N-terminal alanines? I can't seem to find one, but finding anything in "the literature" has become a hit or miss proposition.
December 1, 2025 at 5:11 PM
Does anybody know if an N-degron mechanism has been discovered for proteins with unblocked N-terminal alanines? I can't seem to find one, but finding anything in "the literature" has become a hit or miss proposition.
🌿 Charlene Dambire 🌿
A postdoctoral researcher at the University of Nottingham, Charlene's research on plant oxygen sensing through the N-degron pathway is key to unlocking plant stress responses.
Beyond science, she is a keen advocate for diversity & gender equality in STEM 🧪
#plantscience
A postdoctoral researcher at the University of Nottingham, Charlene's research on plant oxygen sensing through the N-degron pathway is key to unlocking plant stress responses.
Beyond science, she is a keen advocate for diversity & gender equality in STEM 🧪
#plantscience
October 29, 2025 at 3:55 PM
🌿 Charlene Dambire 🌿
A postdoctoral researcher at the University of Nottingham, Charlene's research on plant oxygen sensing through the N-degron pathway is key to unlocking plant stress responses.
Beyond science, she is a keen advocate for diversity & gender equality in STEM 🧪
#plantscience
A postdoctoral researcher at the University of Nottingham, Charlene's research on plant oxygen sensing through the N-degron pathway is key to unlocking plant stress responses.
Beyond science, she is a keen advocate for diversity & gender equality in STEM 🧪
#plantscience
New research led by Maggie Wong (@MPI_NL) reveals that SETBP1 variants outside the degron can disrupt transcription & neuron development - redefining the disease spectrum. Largest cohort to date (n=18). Nature Comms: www.nature.com/articles/s41...
#genetics #SETBP1
#genetics #SETBP1
Client Challenge
www.nature.com
October 14, 2025 at 8:35 AM
New research led by Maggie Wong (@MPI_NL) reveals that SETBP1 variants outside the degron can disrupt transcription & neuron development - redefining the disease spectrum. Largest cohort to date (n=18). Nature Comms: www.nature.com/articles/s41...
#genetics #SETBP1
#genetics #SETBP1
Two new volumes of Methods in Enzymology with 29 chapters/papers on Protein termini methods and reviews are now out! Thx to all authors!🙌 @ispt-proteinterm.bsky.social #N-degron #prenylation #myristoylation #formylation #arginylation #oxidation #acetylation www.sciencedirect.com/bookseries/m...
Methods in Enzymology | Volume 719: Protein Termini - Part B | ScienceDirect.com by Elsevier
Read the latest chapters of Methods in Enzymology at ScienceDirect.com, Elsevier’s leading platform of peer-reviewed scholarly literature
www.sciencedirect.com
September 25, 2025 at 9:17 AM
Two new volumes of Methods in Enzymology with 29 chapters/papers on Protein termini methods and reviews are now out! Thx to all authors!🙌 @ispt-proteinterm.bsky.social #N-degron #prenylation #myristoylation #formylation #arginylation #oxidation #acetylation www.sciencedirect.com/bookseries/m...
Does inhibition of the insulin receptor DAF-2 during starvation inhibit feeding behavior by activating the transcription factor DAF-16/FoxO in enteric neurons? To test this, we removed DAF-16/FoxO from the ENS using the auxin-inducible degron (AID) technology.
(9/n)
(9/n)
September 24, 2025 at 8:31 PM
Does inhibition of the insulin receptor DAF-2 during starvation inhibit feeding behavior by activating the transcription factor DAF-16/FoxO in enteric neurons? To test this, we removed DAF-16/FoxO from the ENS using the auxin-inducible degron (AID) technology.
(9/n)
(9/n)
Developing leaves form a spatiotemporal oxygen gradient that is sensed through the oxygen sensing machinery and directs morphogenesis | New preprint from @daanweits.bsky.social and @syno2xis.bsky.social labs #N-degron #Cysteine #hypoxia @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...
September 23, 2025 at 7:38 AM
Developing leaves form a spatiotemporal oxygen gradient that is sensed through the oxygen sensing machinery and directs morphogenesis | New preprint from @daanweits.bsky.social and @syno2xis.bsky.social labs #N-degron #Cysteine #hypoxia @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...
We link the PCO N-degron pathway to acquisition of specialized cell fates and cell expansion, resulting in an altered leaf shape. A detailed RNAseq analysis led us to identify key genes downstream of the oxygen sensing pathway involved in the formation of specialized cells in leaves.
September 22, 2025 at 6:52 AM
We link the PCO N-degron pathway to acquisition of specialized cell fates and cell expansion, resulting in an altered leaf shape. A detailed RNAseq analysis led us to identify key genes downstream of the oxygen sensing pathway involved in the formation of specialized cells in leaves.
Anatomy and habitat shape the oxygen sensing machinery of flowering plants | new preprint from Licausi lab @syno2xis.bsky.social #hypoxia #N-degron @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...
Anatomy and habitat shape the oxygen sensing machinery of angiosperms
Hypoxia sensing via the Cys/Arg branch of the N-degron pathway (Cys-NDP) is central for flooding responses in plants, yet how evolutionary and ecological factors have shaped the core oxygen sensing me...
www.biorxiv.org
September 18, 2025 at 12:40 PM
Anatomy and habitat shape the oxygen sensing machinery of flowering plants | new preprint from Licausi lab @syno2xis.bsky.social #hypoxia #N-degron @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...
New review on the generation and consequences of N-terminal proteoform diversity from Evan Morrison and Olivia Rissland | #N-degron #RNA @cp-cellreports.bsky.social www.sciencedirect.com/science/arti...
The generation and consequences of N-terminal proteoform diversity
N-terminal proteoforms are protein variants with altered N termini that arise from RNA-driven processes, such as alternative promoter usage, splicing,…
www.sciencedirect.com
September 17, 2025 at 8:09 AM
New review on the generation and consequences of N-terminal proteoform diversity from Evan Morrison and Olivia Rissland | #N-degron #RNA @cp-cellreports.bsky.social www.sciencedirect.com/science/arti...
Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform | Yong Tae Kwon lab | #N-degron www.biorxiv.org/content/10.1...
Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of motor neurons and the cytoplasmic aggregation of misfolded proteins in the spinal cord...
www.biorxiv.org
September 8, 2025 at 11:07 AM
Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform | Yong Tae Kwon lab | #N-degron www.biorxiv.org/content/10.1...
A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants | Preprint from @mikellavi.bsky.social @beagiuntoli.bsky.social @syno2xis.bsky.social #N-degron #hypoxia www.biorxiv.org/content/10.1...
A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants
Plants face hypoxic conditions either chronically, as particular tissues are characterized by fluctuating or stable low oxygen levels, or acutely, when flooded. In vascular plants, transcriptional ada...
www.biorxiv.org
September 8, 2025 at 10:15 AM
A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants | Preprint from @mikellavi.bsky.social @beagiuntoli.bsky.social @syno2xis.bsky.social #N-degron #hypoxia www.biorxiv.org/content/10.1...
The Cys N-Degron Pathway (which controls plant responses to hypoxia) works in coordination with many, many other processes that finely tune low O2 responses.
We took it.
Put it in yeast, where no such regulation exists.
And it still works! So the Cys-NDP is self-sufficient...
We took it.
Put it in yeast, where no such regulation exists.
And it still works! So the Cys-NDP is self-sufficient...
September 5, 2025 at 4:32 PM
The Cys N-Degron Pathway (which controls plant responses to hypoxia) works in coordination with many, many other processes that finely tune low O2 responses.
We took it.
Put it in yeast, where no such regulation exists.
And it still works! So the Cys-NDP is self-sufficient...
We took it.
Put it in yeast, where no such regulation exists.
And it still works! So the Cys-NDP is self-sufficient...
Targeted degradation of SARS-CoV-2 via the autophagy-lysosome system using chemical mimetics of the N-degron pathway https://www.biorxiv.org/content/10.1101/2025.09.01.673409v1
September 3, 2025 at 3:17 AM
Targeted degradation of SARS-CoV-2 via the autophagy-lysosome system using chemical mimetics of the N-degron pathway https://www.biorxiv.org/content/10.1101/2025.09.01.673409v1
Targeted degradation of SARS-CoV-2 via the autophagy-lysosome system using chemical mimetics of the N-degron pathway https://www.biorxiv.org/content/10.1101/2025.09.01.673409v1
September 3, 2025 at 3:17 AM
Targeted degradation of SARS-CoV-2 via the autophagy-lysosome system using chemical mimetics of the N-degron pathway https://www.biorxiv.org/content/10.1101/2025.09.01.673409v1
Comment in issue 17: Making sense of oxygen sensing
Glutamine sensing by mTORC1 via GCN2
Androgen signaling control via Parp7 and an ADP-ribosyl degron
Dendrite-like pseudopods let macrophages ingest bacteria
Cover by @miguelgf.bsky.social et al
www.embopress.org/toc/14602075...
Glutamine sensing by mTORC1 via GCN2
Androgen signaling control via Parp7 and an ADP-ribosyl degron
Dendrite-like pseudopods let macrophages ingest bacteria
Cover by @miguelgf.bsky.social et al
www.embopress.org/toc/14602075...
September 2, 2025 at 9:37 AM
Comment in issue 17: Making sense of oxygen sensing
Glutamine sensing by mTORC1 via GCN2
Androgen signaling control via Parp7 and an ADP-ribosyl degron
Dendrite-like pseudopods let macrophages ingest bacteria
Cover by @miguelgf.bsky.social et al
www.embopress.org/toc/14602075...
Glutamine sensing by mTORC1 via GCN2
Androgen signaling control via Parp7 and an ADP-ribosyl degron
Dendrite-like pseudopods let macrophages ingest bacteria
Cover by @miguelgf.bsky.social et al
www.embopress.org/toc/14602075...
Mechanistically, NMTi-induced mitochondrial failure is concurrent with loss of myristoylation on respiratory complex I assembly factor NDUFAF4 with its subsequent proteasomal degradation via the glycine N-degron mechanism.
August 28, 2025 at 3:22 PM
Mechanistically, NMTi-induced mitochondrial failure is concurrent with loss of myristoylation on respiratory complex I assembly factor NDUFAF4 with its subsequent proteasomal degradation via the glycine N-degron mechanism.
Degradation of Abl Utilizing an Immunoproteasome N-Degron Prodrug | Journal of Medicinal Chemistry pubs.acs.org/doi/10.1021/...
Degradation of Abl Utilizing an Immunoproteasome N-Degron Prodrug
Targeted protein degradation is an emerging therapeutic strategy that leverages the cell’s natural protein clearance pathways to eliminate proteins of interest (POIs). One common approach involves bif...
pubs.acs.org
August 24, 2025 at 10:37 PM
Degradation of Abl Utilizing an Immunoproteasome N-Degron Prodrug | Journal of Medicinal Chemistry pubs.acs.org/doi/10.1021/...
Structural basis for the recognition and ubiquitylation of type-2 N-degron substrate by PRT1 plant N-recognin
nature.com/articles/s41...
#plantscience
nature.com/articles/s41...
#plantscience
August 23, 2025 at 5:13 PM
Structural basis for the recognition and ubiquitylation of type-2 N-degron substrate by PRT1 plant N-recognin
nature.com/articles/s41...
#plantscience
nature.com/articles/s41...
#plantscience
Cool paper! This also one my hypotheses during my PhD , but we have never checked in plants 🌱🌊
Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability
www.pnas.org/doi/10.1073/...
Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability
www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
August 20, 2025 at 2:23 PM
Cool paper! This also one my hypotheses during my PhD , but we have never checked in plants 🌱🌊
Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability
www.pnas.org/doi/10.1073/...
Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability
www.pnas.org/doi/10.1073/...
Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | Important finding by Kim, Tian, Ratcliffe and Keeley @ox.ac.uk @ispt-proteinterm.bsky.social #N-degron www.pnas.org/doi/10.1073/...
Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | PNAS
Selected proteins containing an N-terminal cysteine (Nt-Cys) are subjected to rapid,
O2-dependent proteolysis via the Cys/Arg-branch of the N-degro...
www.pnas.org
August 20, 2025 at 10:39 AM
Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | Important finding by Kim, Tian, Ratcliffe and Keeley @ox.ac.uk @ispt-proteinterm.bsky.social #N-degron www.pnas.org/doi/10.1073/...
How to define degrons and E3 ligase specificities by peptide pulldowns | Methods in Enzymology | Kwon and Ji labs | #KCMF1 #Cysteine #Arg/N-degron @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
August 7, 2025 at 7:11 AM
How to define degrons and E3 ligase specificities by peptide pulldowns | Methods in Enzymology | Kwon and Ji labs | #KCMF1 #Cysteine #Arg/N-degron @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...