dave dougan
@douganlab.bsky.social
Regulated proteolysis | N-degron | AAA+ machines | #Proteostasis #Ndegron https://scholar.google.com/citations?user=6LL-q08AAAAJ&hl=en … views my own
Reposted by dave dougan
🏔️🌧️ 🧬 We wrote this Expert View on oxygen sensing, you can read it here! 👇🏼
Recent advances in the mechanism, transduction and function of oxygen sensing in plants
#nendrule #hypoxia #plantscience
academic.oup.com/jxb/advance-...
#nendrule #hypoxia #plantscience
academic.oup.com/jxb/advance-...
Recent advances in the mechanism, transduction and function of oxygen sensing in plants
Abstract. Response to reduced ambient oxygen is a key component of plant adaptation in natural and farmed environments and is regulated by oxygen dependent
academic.oup.com
November 23, 2025 at 1:21 PM
🏔️🌧️ 🧬 We wrote this Expert View on oxygen sensing, you can read it here! 👇🏼
Reposted by dave dougan
How does the kinase ZAK sense ribosome collisions? Find out in our latest collaboration with the @greenlab.bsky.social @doubleshuang.bsky.social @Vienna Huso: 1/4
rdcu.be/eRmJl
#ribosome #cryoEM #LMU #JHMI
rdcu.be/eRmJl
#ribosome #cryoEM #LMU #JHMI
November 23, 2025 at 3:47 PM
How does the kinase ZAK sense ribosome collisions? Find out in our latest collaboration with the @greenlab.bsky.social @doubleshuang.bsky.social @Vienna Huso: 1/4
rdcu.be/eRmJl
#ribosome #cryoEM #LMU #JHMI
rdcu.be/eRmJl
#ribosome #cryoEM #LMU #JHMI
Reposted by dave dougan
Through proteomic profiling of our proprietary compound library, our team identified a selective, fast, and potent molecular glue degrader that induces cereblon degradation via a novel mechanism.
Read the full paper in @natcomms.nature.com: rdcu.be/eQKQk
Read the full paper in @natcomms.nature.com: rdcu.be/eQKQk
November 19, 2025 at 3:28 PM
Through proteomic profiling of our proprietary compound library, our team identified a selective, fast, and potent molecular glue degrader that induces cereblon degradation via a novel mechanism.
Read the full paper in @natcomms.nature.com: rdcu.be/eQKQk
Read the full paper in @natcomms.nature.com: rdcu.be/eQKQk
Reposted by dave dougan
Structural basis for co-translational assembly of homo-oligomeric proteins www.biorxiv.org/content/10.1101/2025.11.12.687954v1 #cryoem
November 14, 2025 at 12:14 PM
Structural basis for co-translational assembly of homo-oligomeric proteins www.biorxiv.org/content/10.1101/2025.11.12.687954v1 #cryoem
Reposted by dave dougan
📝 Online now - the Spotlight "Unlocking the protein code: how our organs age across a lifetime" from Dongxue Wang and Jing Yang.
#Proteostasis #Aging #BiologicalClocks #Proteomics
Read it here: authors.elsevier.com/a/1m7Ts3S6Gf...
#Proteostasis #Aging #BiologicalClocks #Proteomics
Read it here: authors.elsevier.com/a/1m7Ts3S6Gf...
November 18, 2025 at 9:35 PM
📝 Online now - the Spotlight "Unlocking the protein code: how our organs age across a lifetime" from Dongxue Wang and Jing Yang.
#Proteostasis #Aging #BiologicalClocks #Proteomics
Read it here: authors.elsevier.com/a/1m7Ts3S6Gf...
#Proteostasis #Aging #BiologicalClocks #Proteomics
Read it here: authors.elsevier.com/a/1m7Ts3S6Gf...
Ribonuclease toxin RelE1 inhibits growth of Mycobacterium tuberculosis through specific cleavage of the ribosomal anti-Shine–Dalgarno region: academic.oup.com/nar/article/...
Validate User
academic.oup.com
November 18, 2025 at 11:36 PM
Ribonuclease toxin RelE1 inhibits growth of Mycobacterium tuberculosis through specific cleavage of the ribosomal anti-Shine–Dalgarno region: academic.oup.com/nar/article/...
Development of a targeted BioPROTAC degrader selective for misfolded SOD1
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Development of a targeted BioPROTAC degrader selective for misfolded SOD1 - Nature Communications
Conventional PROTACs have limited capacity for misfolded proteins. Here, authors develop a BioPROTAC containing an scFv specifically targeting misfolded SOD1, fused to an E3 ligase, and demonstrate a ...
www.nature.com
November 18, 2025 at 11:33 PM
Development of a targeted BioPROTAC degrader selective for misfolded SOD1
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by dave dougan
A fluorescent probe enables direct visualization and measurement of membrane tension in live bacteria, offering new insights into how bacteria interact with and respond to different surfaces. doi.org/hbbsxk
How bacteria 'feel' surfaces: Fluorescent probe visualizes and quantifies membrane tension
In natural environments, bacteria rarely live as free-swimming cells but are attached to surfaces as biofilms in medical devices, mobile phones or human tissue.
phys.org
November 18, 2025 at 8:02 PM
A fluorescent probe enables direct visualization and measurement of membrane tension in live bacteria, offering new insights into how bacteria interact with and respond to different surfaces. doi.org/hbbsxk
Reposted by dave dougan
ICYMI: New online: Structural landscape of the degrading 26S proteasome reveals conformation-specific binding of TXNL1
Structural landscape of the degrading 26S proteasome reveals conformation-specific binding of TXNL1
Nature Structural & Molecular Biology, Published online: 06 November 2025; doi:10.1038/s41594-025-01695-2The authors use time-resolved cryo-electron microscopy to reveal the interactions of the redox-active cofactor TXNL1 with the human 26S proteasome and detect ATPase motor states that indicate burst-like mechanisms for hand-over-hand substrate translocation.
go.nature.com
November 7, 2025 at 11:45 AM
ICYMI: New online: Structural landscape of the degrading 26S proteasome reveals conformation-specific binding of TXNL1
Reposted by dave dougan
The deubiquitinating enzyme Otu1 releases substrates from the conserved initiation complex of the Cdc48/p97 ATPase for proteasomal degradation www.biorxiv.org/content/10.1101/2025.11.08.687396v1 #cryoem
November 10, 2025 at 1:56 PM
The deubiquitinating enzyme Otu1 releases substrates from the conserved initiation complex of the Cdc48/p97 ATPase for proteasomal degradation www.biorxiv.org/content/10.1101/2025.11.08.687396v1 #cryoem
How Pathogens Maintain Proteostasis During Infection
onlinelibrary.wiley.com/doi/10.1111/...
onlinelibrary.wiley.com/doi/10.1111/...
How Pathogens Maintain Proteostasis During Infection
Differential ribosome association of protein chaperones trigger factor and DnaK under sufficient and low cytoplasmic Mg2+ conditions. Trigger factor (TF) associates with ribosomes and cotranslational...
onlinelibrary.wiley.com
November 10, 2025 at 3:59 AM
How Pathogens Maintain Proteostasis During Infection
onlinelibrary.wiley.com/doi/10.1111/...
onlinelibrary.wiley.com/doi/10.1111/...
In situ characterization of mitochondrial Hsp60-Hsp10 chaperone complex under folding stress | Science Advances www.science.org/doi/10.1126/...
In situ characterization of mitochondrial Hsp60-Hsp10 chaperone complex under folding stress
The mitochondrial Hsp60-Hsp10 complex clusters and shifts conformation in response to protein-folding stress.
www.science.org
October 27, 2025 at 12:17 AM
In situ characterization of mitochondrial Hsp60-Hsp10 chaperone complex under folding stress | Science Advances www.science.org/doi/10.1126/...
Reposted by dave dougan
Joel Mokyr, Philippe Aghion and Pewter Howitt share economics prize for work that underlines the importance of investing in research and development.
go.nature.com/4hj9NNV
go.nature.com/4hj9NNV
Economics Nobel prize won by researchers who showed how science boosts growth
Joel Mokyr, Philippe Aghion and Pewter Howitt share economics prize for work that underlines the importance of investing in research and development.
go.nature.com
October 13, 2025 at 8:37 PM
Joel Mokyr, Philippe Aghion and Pewter Howitt share economics prize for work that underlines the importance of investing in research and development.
go.nature.com/4hj9NNV
go.nature.com/4hj9NNV
Arctic seals face extinction and birds in decline globally, but there is some good news about green sea turtles www.cnn.com/2025/10/10/s...
Arctic seals face extinction and birds in decline globally, according to new Red List | CNN
More than a quarter of all animals, plants and fungi assessed are at risk of extinction, according to the latest IUCN Red List of Threatened Species.
www.cnn.com
October 13, 2025 at 11:31 PM
Arctic seals face extinction and birds in decline globally, but there is some good news about green sea turtles www.cnn.com/2025/10/10/s...
Well-exercised male mice appear to pass fitness to their male offspring | Science | AAAS www.science.org/content/arti...
Well-exercised male mice appear to pass fitness to their male offspring
Surprising epigenetic effect relies on snippets of RNA packaged within sperm
www.science.org
October 9, 2025 at 3:28 AM
Well-exercised male mice appear to pass fitness to their male offspring | Science | AAAS www.science.org/content/arti...
Reposted by dave dougan
Structural polymorphism of α-synuclein fibrils alters the pathway of Hsc70-mediated disaggregation | The EMBO Journal www.embopress.org/doi/full/10....
Structural polymorphism of α-synuclein fibrils alters the pathway of Hsc70-mediated disaggregation | The EMBO Journal
imageimageIn synucleinopathies such as Parkinson’s disease, the intrinsically disordered protein
α-synuclein aggregates into amyloid fibrils. This study explores the effect of structural
polymorphism ...
www.embopress.org
October 6, 2025 at 1:41 PM
Structural polymorphism of α-synuclein fibrils alters the pathway of Hsc70-mediated disaggregation | The EMBO Journal www.embopress.org/doi/full/10....
Reposted by dave dougan
Out in Science Advances: Our #cryoEM structure of HFTV1, a virus infecting the halophile #archaea. *First full atomic structure (containing all structural proteins) of any tailed virus!* Congrats and thanks to all co-authors and our fantastic collaborators! www.science.org/doi/10.1126/...
Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail
This structure of an archaeal tailed virus (arTV) provides detailed insights into arTV assembly and infection mechanisms.
www.science.org
October 6, 2025 at 11:31 AM
Out in Science Advances: Our #cryoEM structure of HFTV1, a virus infecting the halophile #archaea. *First full atomic structure (containing all structural proteins) of any tailed virus!* Congrats and thanks to all co-authors and our fantastic collaborators! www.science.org/doi/10.1126/...
Reposted by dave dougan
Dr Jane Goodall the name fits @2bdynamic.bsky.social @booksstlouis.bsky.social
Dr. Jane Goodall filmed an interview with Netflix in March 2025 that she understood would only be released after her death.
October 6, 2025 at 6:24 PM
Dr Jane Goodall the name fits @2bdynamic.bsky.social @booksstlouis.bsky.social
Single Particle Dynamics of Protein Aggregation and Disaggregation in the Presence of the sHsp Proteins IbpAB | Biochemistry pubs.acs.org/doi/10.1021/...
Single Particle Dynamics of Protein Aggregation and Disaggregation in the Presence of the sHsp Proteins IbpAB
The small heat shock proteins (sHsps) are a key class of molecular chaperones that can inhibit protein aggregation and enhance protein recovery from aggregates. However, the mechanisms sHsps employ to...
pubs.acs.org
October 7, 2025 at 12:19 AM
Single Particle Dynamics of Protein Aggregation and Disaggregation in the Presence of the sHsp Proteins IbpAB | Biochemistry pubs.acs.org/doi/10.1021/...
Reposted by dave dougan
Let's all do what we can to encourage smokers to quit & others to never start. @booksstlouis.bsky.social @2bdynamic.bsky.social @melrea.bsky.social @piperformissouri.bsky.social @anneserling.bsky.social @rodserlingmemfndtn.bsky.social @thenightgallery.bsky.social @morgfair.bsky.social
October 6, 2025 at 4:40 AM
Let's all do what we can to encourage smokers to quit & others to never start. @booksstlouis.bsky.social @2bdynamic.bsky.social @melrea.bsky.social @piperformissouri.bsky.social @anneserling.bsky.social @rodserlingmemfndtn.bsky.social @thenightgallery.bsky.social @morgfair.bsky.social
And now for something completely different in co-translational targeting: Molecular Cell www.cell.com/molecular-ce...
And now for something completely different in co-translational targeting
In two recent papers in Cell, Zhu et al. and Luo et al. use selective ribosome profiling
to identify the substrates and timing of co-translational protein targeting to mitochondria.
The results sugges...
www.cell.com
October 6, 2025 at 4:41 AM
And now for something completely different in co-translational targeting: Molecular Cell www.cell.com/molecular-ce...
Reposted by dave dougan
Nuclear and cytosolic J-domain proteins provide synergistic control of Hsf1 at distinct phases of the heat shock response
elifesciences.org/articles/107...
elifesciences.org/articles/107...
Nuclear and cytosolic J-domain proteins provide synergistic control of Hsf1 at distinct phases of the heat shock response
The nuclear J-domain protein Apj1 controls the shut-off phase of the yeast heat shock response by displacing DNA-bound heat shock transcription factor Hsf1.
elifesciences.org
October 5, 2025 at 8:38 AM
Nuclear and cytosolic J-domain proteins provide synergistic control of Hsf1 at distinct phases of the heat shock response
elifesciences.org/articles/107...
elifesciences.org/articles/107...
Reposted by dave dougan
New online: Cryo-EM structures reveal the molecular mechanism of SUMO E1–E2 thioester transfer
Cryo-EM structures reveal the molecular mechanism of SUMO E1–E2 thioester transfer
Nature Structural & Molecular Biology, Published online: 25 September 2025; doi:10.1038/s41594-025-01681-8This study reveals how human small ubiquitin-like modifier (SUMO) E1 recruits its E2 partner UBC9 and transfers SUMO1 through large structural changes, uncovering key mechanisms that ensure specificity and fidelity in SUMOylation, an essential protein modification pathway.
go.nature.com
September 25, 2025 at 11:59 AM
New online: Cryo-EM structures reveal the molecular mechanism of SUMO E1–E2 thioester transfer
Reposted by dave dougan
Allosteric control of the bacterial ClpC/ClpP protease and its hijacking by antibacterial peptides
Axel Mogk, Marta Carroni et al
www.embopress.org/doi/full/10....
Axel Mogk, Marta Carroni et al
www.embopress.org/doi/full/10....
September 29, 2025 at 11:56 AM
Allosteric control of the bacterial ClpC/ClpP protease and its hijacking by antibacterial peptides
Axel Mogk, Marta Carroni et al
www.embopress.org/doi/full/10....
Axel Mogk, Marta Carroni et al
www.embopress.org/doi/full/10....
Reposted by dave dougan
Ubiquitin precursor with C-terminal extension promotes proteostasis and longevity
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Ubiquitin precursor with C-terminal extension promotes proteostasis and longevity
Ubiquitin is a conserved modifier regulating the stability and function of numerous target proteins. In all eukaryotes, polyubiquitin precursors are g…
www.sciencedirect.com
September 25, 2025 at 11:03 AM
Ubiquitin precursor with C-terminal extension promotes proteostasis and longevity
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...