European Journal of Human Genetics - Multiple lesion-specific somatic mutations and bi-allelic loss of ACVRL1 in a single patient with hereditary haemorrhagic telangiectasia

Postdoctoral Research Associate in vascular cell plasticity and translation (single-cell and omics) (Fixed Term) We invite applicants for a postdoc position (Research Associate) for a project in Dr

Endothelial cells (ECs) play a critical role in managing vascular homeostasis and neovascularization. EC functions vary significantly depending on their anatomic locations, especially for ECs forming macrovascular versus microvascular vessels. ECs possess heterogeneous signaling pathways, energy metabolism, and cellular behaviors that enable them to handle both physiological and hyperglycemic conditions. These variations can impact the efficacy of pharmacotherapy and influence the likelihood of unexpected side effects. In this study, we compared human aortic ECs (HAECs) and human dermal microvascular ECs (HDMVECs) to observe the functional and proteomic differences potentially contributing to EC heterogeneity. Compared to HAECs, HDMVECs exhibited faster proliferation, but lower migration and permeability. Under high glucose, migration was worsened for both cell types, whereas proliferation was unaffected, and permeability increased for HDMVECs. Using proteomic analysis, we identified 126 proteins whose abundance was significantly different between HAECs and HDMVECs. DAVID analysis revealed their biological processes, cellular compartments, molecular functions, and pathways. under high glucose, WARS1 increased while SOD2 decreased. Reversing WARS1 or SOD2 expression levels improved HDMVEC migration and permeability functions. The combined treatment of WARS1 knockdown and SOD2 overexpression fully restored EC migration and reduced permeability to levels comparable to those of their counterparts under normal glucose conditions. Furthermore, in the cutaneous wound in type 2 diabetic mice, the combination therapy of Wars1 knockdown and SOD2 overexpression accelerated the wound closure and augmented wound angiogenesis. Our studies provide novel molecular insights into EC heterogeneity and identified WARS1 and SOD2 as potential targets for dermal angiogenesis during tissue repair.

Cyclic stretch, generated by pulsatile blood pressure, has been identified as a pivotal regulator of vascular smooth muscle cell (VSMC) behavior and arterial wall homeostasis. Research has demonstrate...

The transcriptional co-activators YAP (YAP1) and TAZ (WWTR1) are central regulators of vascular smooth muscle cell (VSMC) phenotype and vascular homeo…

Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is one of the most severe complications of antiresorptive therapy. Although impaired angiogenesis is commonly observed in BRONJ, the underlying ...

Karger Cardiovascular System covers a wide spectrum of topics in basic and clinical research, which are also connected to Nephrology and Neurology in some of our journals