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Boris Louis 🧪🔬 @borislouis.bsky.social · 14d

Microscopy building day 7-9
First images and final checks

We finally have a working microscope 🔬

After installing the camera, we tackled hardware software communications using @micro-manager.bsky.social

Then we performed usual checks:
1) fluorescent marker
2) scale bar
3) bead solution (PSF)

Reposted by Boris Louis 🧪🔬

Christophe 🔬 L @christlet.bsky.social · Apr 9

We have a new review out! In this Microscopy & Microanalysis piece, Louisa Mezache and I survey some of the latest commercially available microscope technologies: Nikon NSPARC, Re-scan Gaia, CSR Biotech MI-SIM, refinements to STED, SMLM from Bruker and Abbelight… Check it out with free access:

Advancing Super-Resolution Microscopy: Recent Innovations in Commercial Instruments

Abstract. Super-resolution microscopy techniques have accelerated scientific progress, enabling researchers to explore cellular structures and dynamics wit

academic.oup.com

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Reposted by Boris Louis 🧪🔬

Nature Methods @natmethods.nature.com · May 14

A universal AI-powered pipeline enables precise 3D analysis of cell morphology and topology within organoid structures.

www.nature.com/articles/s41...

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Reposted by Boris Louis 🧪🔬

Stacey Ogden 🦔 @labogden.bsky.social · May 15

I never imagined I would see something like this happen to federally funded research in the US. I am heartbroken for all the researchers whose grants have been terminated and devastated for all the patients whose current (and future) treatments are being impacted 🧪 💔

Craig Kaplan @triggerloop.bsky.social · May 15

Current situation at Harvard Medical School for NIH grants view.u.hms.harvard.edu?qs=3ed8c01b9...

Harvard Medical School

view.u.hms.harvard.edu

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 15

How we analyze porous network @rochalab.bsky.social 🔬🧪

1) Adaptive thresholding
2)Distance map calculation
3)Watershed transformation
4)Quantification
5)3D rendering

Code : github.com/BorisLouis/S...

Paper: pubs.rsc.org/en/content/a...

#Microscopy #Optics #SciComm #ImageProcessing

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 6

An interesting article from Microscopy and Analysis (by Wiley Analytical science)🧪🔬

Useful checklist if you are looking to build a new optical system or currently improving one !

analyticalscience.wiley.com/content/arti...

Practical considerations for building optical setups - 2025 - Wiley Analytical Science

The largest repository of validated, free and subject-focused e-publications and online seminars in analytical science covering latest techniques, equipment, original research, editorials, and industr...

analyticalscience.wiley.com

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 6

Good observation! Yes in this state it would be an issue, we just finished the alignment, we will run a few test and check the imaging before finalizing the setup which include making protection around the system 🙏

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 5

I think one is from Saruman and the other from Sauron?
Not sure what is the use of Saruman's one though 😅

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Reposted by Boris Louis 🧪🔬

Dr. Lucky Tran @luckytran.com · Apr 28

Deaths from heart disease down 75%, that’s NIH.

Deaths from stroke down 75%, that’s NIH.

HIV/AIDS no longer a death sentence, that’s NIH.

99% of FDA approved drugs in the last decade, that’s NIH.

Please show this video to anyone who doesn’t understand why the NIH is so important.

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 5

1) two vertical support rods, simple Thorlabs posts that reminded us the Two Towers from Lord of the Rings.

2) A fully caged system giving a high rigidity to the setup and reinforcing the stability of the parts that are hanging in the air.

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 5

But going upright comes with challenges, not only because it was our first time building such microscope:

📏 The objective lens has to be placed significantly higher to leave room for a sample stage below and potential illumination sources.

💥 That makes stability a real issue.

⚙️ Our solution:

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 5

Most research microscopes are inverted

𝐒𝐨 𝐰𝐡𝐲 𝐮𝐩𝐫𝐢𝐠𝐡𝐭?

Because we’re working with photonic chips in which we have to couple an optical fiber in, quite accurately which is not possible with the sample upside down.

... and that means flipping the whole optical setup upside-down (literally).

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 5

🧙‍♂️𝐋𝐨𝐫𝐝 𝐨𝐟 𝐭𝐡𝐞 𝐑𝐢𝐧𝐠𝐬: 𝐓𝐡𝐞 𝐓𝐰𝐨 𝐓𝐨𝐰𝐞𝐫𝐬 – 𝐌𝐢𝐜𝐫𝐨𝐬𝐜𝐨𝐩𝐞 𝐄𝐝𝐢𝐭𝐢𝐨𝐧 🔬 🧪

Meet our latest creation @hofkenslab.bsky.social and @rochalab.bsky.social labs:

A fully custom upright microscope built entirely from scratch, with no microscope body and no commercial frame.

#Optics #SciComm #Microscopy

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 4

Their prototype uses a retractable plasma blade fuelled by compressed liquid propane and oxygen, reaching temperatures around 4,000°F, hot enough to cut through steel. 🤯 While it's not yet practical for duelling Sith Lords, it's a remarkable engineering feat.

Boris Louis 🧪🔬 @borislouis.bsky.social · May 4

𝐌𝐚𝐲 𝐭𝐡𝐞 𝟒𝐭𝐡 𝐁𝐞 𝐖𝐢𝐭𝐡 𝐘𝐨𝐮 🔦⚔️

On this Star Wars Day, I revisited Hacksmith Entertainment Ltd. impressive attempt at creating a real-life lightsaber. If you are into starwars and science, you will probably have seen it !

youtube.com/clip/UgkxTLb...

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Reposted by Boris Louis 🧪🔬

Alfred Millett-Sikking @amsikking.bsky.social · May 3

This looks awesome! A new open source optical design software! 😱

Has anyone used it? Is it good? 😁

optiland.readthedocs.io/en/latest/in...

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 1

Having both high resolution structural information 🔬and functional information 🧪 (in this case, tension) is very challenging, very cool work!
#Microscopy #optics

Isaac Li @isaacli.bsky.social · Apr 18

Check out our recent progress using 🧬 PAINT kinetics to decode molecular tension in super-resolution force imaging! 🔬 Great team effort from Seongho, Adam, Irving, and Kevin! 👏
#DNA-PAINT #tension #integrin #superresolution #adhesion

doi.org/10.1002/advs...

Quantitative Super‐Resolution Imaging of Molecular Tension

Quantitative tension PAINT (qtPAINT) is a new method to quantify molecular tension at super-resolution by exploiting force-dependent DNA oligonucleotide dissociation kinetics. Combining hairpin tensi....

doi.org

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 1

This work was done in @hofkenslab.bsky.social and Martin Vacha's lab and lead by @sudiptaseth.bsky.social.

We combine structural information obtained by SEM with functional information obtained by PL and our correlation clustering imaging.

Here is the link to the paper pubs.acs.org/doi/10.1021/...

Unveiling the Local Fate of Charge Carriers in Halide Perovskite Thin Films via Correlation Clustering Imaging

As the field of metal halide perovskites matures, a range of compositionally different perovskite films has found a place in efficient optoelectronic devices. These films feature variable local struct...

pubs.acs.org

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 1

🔬Interested in imaging in energy science ?🔬

Chemical & Biomedical Imaging, a new journal from @acs.org, invited us for a special issue on

🔬𝐈𝐦𝐚𝐠𝐢𝐧𝐠 𝐢𝐧 𝐄𝐧𝐞𝐫𝐠𝐲 𝐬𝐜𝐢𝐞𝐧𝐜𝐞 ☀️ ⚡ 🧪

And we made the cover ! 🎊

Thanks to @alexeychizhik.bsky.social for the amazing design

#Microscopy #Optics #SciComm

Correlative imaging, Functional imaging, photoactive material, Correlation clustering imaging

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Boris Louis 🧪🔬 @borislouis.bsky.social · May 1

Thanks a lot 😇

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Reposted by Boris Louis 🧪🔬

Nature @nature.com · Apr 30

This week on the Nature Podcast: In the face of massive upheaval, what is the long-term outlook for science in the US and beyond?

https://go.nature.com/4k0pdH6

The dismantling of US science: can it survive Trump 2.0?

Hear the biggest stories from the world of science | 30 April 2025

go.nature.com

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Boris Louis 🧪🔬 @borislouis.bsky.social · Apr 30

Hello Prof. Grange!

Would love to be included, here is my google scholar:
scholar.google.com/citations?us...

Also started to post about building of optical setup on this platform.

Boris Louis 🧪🔬 @borislouis.bsky.social · Apr 30

Hello Mike,

Would love to join if there is still space !
Here is my google scholar: scholar.google.com/citations?us...

Also started to post about microscopy here

Boris Louis 🧪🔬 @borislouis.bsky.social · Apr 30

Hey, would love to be part of it
Here is my google scholar
scholar.google.com/citations?us...
I am also starting to post regularly on microscopy building and data analysis

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Boris Louis 🧪🔬 @borislouis.bsky.social · Apr 30

Looking forward, will be in U.S. For the first time 🙏