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dyneinassembly.bsky.social
(GIRISH)MALI LAB
@dyneinassembly.bsky.social
1.1K followers 370 following 71 posts
Group Leader/MRC CDA Fellow (Dunn School of Pathology, University of Oxford)

https://www.path.ox.ac.uk/research-group/girish-ram-mali/
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dyneinassembly.bsky.social
(GIRISH)MALI LAB @dyneinassembly.bsky.social · Sep 29
Happy to share the inaugural paper from the lab. We describe a molecular mechanism for the activation of outer dynein arm motors that power the vital motion of cilia.

Open access link below:
www.nature.com/articles/s41...

Here's a cool animated summary
Reposted by (GIRISH)MALI LAB
focalplane.bsky.social
We’re excited for the UK Cilia and Centrosome Network e-symposium this afternoon featuring the #JCSciliaSI!

To discover more about the research in this issue, check out these JCS snapshots, video highlights from authors about their research: focalplane.biologists.com/2025/11/24/j...
JCS snapshots: the cilia and flagella edition - FocalPlane
JCS snapshots: the cilia and flagella edition - News
focalplane.biologists.com
November 25, 2025 at 12:29 PM
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thurstonlab.bsky.social
So proud of the lab for its achievements - Thank you to the Pettenkofer foundation for recognising our contributions - here’s to some more bacterial effector related fun in the future! @dunnschool.bsky.social
November 23, 2025 at 9:43 PM
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zjmaggiexu.bsky.social
Thrilled to share our work on the 🔥 single-celled predator Podophrya collini, which rewires its cell morphology to hunt more efficiently. Huge thanks to our amazing team—Amy, Lauren, Omaya, Marine, Mari, and especially Scott—for making this shine! ✨
cellraiser.bsky.social Scott Coyle @cellraiser.bsky.social · 7d
How do cells adapt morphology to function? In a 🔥 preprint by @zjmaggiexu.bsky.social , with @dudinlab.bsky.social and @amyweeks.bsky.social , we identify a self-organizing single-cell morphology circuit that optimizes the feeding trap structure of the suctorian P. collini. 🧵 tinyurl.com/4k8nv926
November 18, 2025 at 5:12 PM
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cellraiser.bsky.social
Key transcripts encoded centrin-like proteins, a frequent component of sensory/contractile structures in protists. We visualized tentacle ultrastructure by U-ExM with @dudinlab.bsky.social lab, discovering stunning tip and collar structures 🤯that add structural complexity to new tentacle formation.
November 18, 2025 at 4:15 PM
dyneinassembly.bsky.social
I saw her at the poster session at ASCB 2024! very very impressive work and such a cool model system to study.
cellraiser.bsky.social Scott Coyle @cellraiser.bsky.social · 7d
This work shines because of @zjmaggiexu.bsky.social passion for these cells. She built it all—the cultures, transcriptomes, analyses, modeling and collaborations—from scratch. I can’t begin to express how brilliant and hard-working a scientist she is (and she's 👀 for postdocs!). So proud of her 🥲
Photographs of Zhejing "Maggie" Xu, an extraordinary gifted, hard-working, and brilliant grad student who is as comfortable at the bench or IDE as she is in the field. Truly an amazing individual whose passion for suctorian, protists, and cell biology more generally is in a class all its own
November 19, 2025 at 9:14 AM
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nogaleslab.bsky.social
Excited to share our new preprint in collaboration with Ahmet Yildiz's lab. Check out how our team uncovers a novel binding footprint and motor regulation mechanism for MAP9 Congrats to Burak Cetin and @aryantaheri.bsky.social

www.biorxiv.org/content/10.1...
November 18, 2025 at 10:43 PM
dyneinassembly.bsky.social
Thanks for the shoutout! @jcellsci.bsky.social :)
jcellsci.bsky.social Journal of Cell Science @jcellsci.bsky.social · 8d
In their Perspective article, Muyang Ren, Siham Jaleel, Stephen King & Girish Mali @dyneinassembly.bsky.social highlight the structural diversity of the axonemal dynein assembly factors & their emerging roles in the biology of motile cilia.
#JCSciliaSI
journals.biologists.com/jcs/article/...
Schematic of the dynein assembly pathway and putative roles of DNAAFs that work within it. Axonemal dynein arm motor subunits (heavy chains, intermediate chains and light chains) are assembled in a multi-step dynein assembly pathway involving steps of folding, assembly and transport. DNAAFs are thought to operate at distinct steps. HSP70 proteins likely engage nascent dynein heavy chain, intermediate chain and light chain subunits as they emerge from the ribosome exit tunnel. Specific DNAAFs then likely hand over semi-folded subunits to the HSP90 chaperone and its co-chaperone R2TP complex for further folding and assembly. Many DNAAFs are proposed to function at this step with RUVBL1/2. Pre-assembled ODA motors are finally packaged into an inhibited closed conformation by DNAAF9 (Shulin) for stable attachment to IFT trains for ciliary import. IDAs might undergo similar packaging for IFT transport via currently unknown factors. Genetic variants that block any step of assembly can cause severe motile ciliopathies such as PCD. PTM, post-translational modification.
November 17, 2025 at 7:04 PM
Reposted by (GIRISH)MALI LAB
strontiumpup.bsky.social
If you’re at #sfn25 you definitely don’t want to miss this nanosymposium on cilia, tomorrow from 1-4pm! Come learn about neuronal cilia, they do some pretty cool stuff!
elinakosty.bsky.social ElinaK @elinakosty.bsky.social · Oct 24
Synapses may get all the glory, but join us cilia-philes for our @sfn.org Nanosymposium to learn about the role of cilia in modulating brain activity, behavior, and development.

Sunday November 16th 1-4pm SDCC Room 25A. Thrilled to chair with @konjikusicmia.bsky.social - we'll see you there!
A graphic depicting a brain with an antenna behind it and the large title "Cilia in the brain". The full text is: Join us for a series of talks on: Cilia in the Brain. Sunday November 16, 2025 1:00 PM- 4:00 PM. Sessin # Nano-013, SDCC RM25A, SfN session title: "Primary Cilia Signaling and Function". on the right half an SfN logo is depicted with a list of speakers for this session
November 15, 2025 at 8:26 PM
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mick-lab.bsky.social
Thrilled to share "in situ APEX activation" (iAPEX), a proximity labeling technology for subcellular #proteomics applied to primary #cilia. iAPEX uses an enzymatic cascade, in which a D-amino acid oxidase locally produces H2O2 to activate ascorbate peroxidase.
www.nature.com/articles/s41...
An enzymatic cascade enables sensitive and specific proximity labeling proteomics in challenging biological systems - Nature Communications
Proximity labeling allows proteomic mapping of subcellular structures. Here, Sroka et al. develop in situ APEX activation (iAPEX) to overcome limitations of labeling specificity and H2O2 toxicity, app...
www.nature.com
November 14, 2025 at 8:07 PM
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jcellsci.bsky.social
We're still accepting submissions for our 'Cell Biology of the Nucleus' special issue, which is being guest edited by
@abbybuch.bsky.social and Megan King (@luskinglab.bsky.social).
Submission deadline: 5 January
journals.biologists.com/jcs/pages/nu...
#nucleus #notforprofit #freetopublish
November 13, 2025 at 5:13 PM
dyneinassembly.bsky.social
Chris is a brilliant structural biologist and mentor. Would highly recommend applying!
chillzaa.bsky.social Chris H. Hill @chillzaa.bsky.social · 12d
🚨Attention prospective PhD students🚨
Are you interested in molecular mechanisms, RNA viruses, structural biology or biophysics? 🧬🦠🧪

We have THREE fully-funded PhD projects available for October 2026 entry:
www.findaphd.com/phds/?Keywor...

Please share/repost, and get in touch if you're interested
chris hill PhD Projects, Programmes & Scholarships - 3 PhDs Listed
FindAPhD. Search Funded PhD Projects, Programmes & Scholarships in chris hill. Search for PhD funding, scholarships & studentships in the UK, Europe and around the world.
www.findaphd.com
November 15, 2025 at 10:28 AM
dyneinassembly.bsky.social
Great to see this work out! Congratulations to the team but especially James Stowell and Conny Wu (a real labour of love)
lapassmore.bsky.social Lori Passmore @lapassmore.bsky.social · 10d
This work is now officially published
@natsmb.nature.com
with some fun new analyses included in the revised version. Congratulations to all the authors! @connyyu.bsky.social @rappsilber.bsky.social @lrsinn.bsky.social @fjoreilly.bsky.social www.nature.com/articles/s41...
November 15, 2025 at 10:26 AM
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lapassmore.bsky.social
This work is now officially published
@natsmb.nature.com
with some fun new analyses included in the revised version. Congratulations to all the authors! @connyyu.bsky.social @rappsilber.bsky.social @lrsinn.bsky.social @fjoreilly.bsky.social www.nature.com/articles/s41...
November 15, 2025 at 9:51 AM
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zephyris-science.bsky.social
Very excited to share this one! Our comprehensive structure, genetics and biophysics dissection of which flagellar dyneins and other proteins are needed for flagella to beat and drive cells swimming behaviours: www.biorxiv.org/content/10.1...
November 8, 2025 at 8:42 AM
dyneinassembly.bsky.social
Wonderful to see this comprehensive study out in pre-print! Congratulations to the whole team.
alanbrownhms.bsky.social Alan Brown @alanbrownhms.bsky.social · 15d
Our latest collaborative effort - with Eva Gluenz and @zephyris-science.bsky.social - to understand how the various proteins and complexes of the axoneme contribute to ciliary motility:
matthdoran.bsky.social Matt Doran @matthdoran.bsky.social · 15d
In an eight-person rowing boat, each rower contributes to movement but also has a unique role: balancing, powering, or setting the rhythm and pace. In our latest collaborative work we asked – do the eight dynein “rowers” in #cilia and #flagella operate in the same way?
November 11, 2025 at 12:28 PM
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matthdoran.bsky.social
In an eight-person rowing boat, each rower contributes to movement but also has a unique role: balancing, powering, or setting the rhythm and pace. In our latest collaborative work we asked – do the eight dynein “rowers” in #cilia and #flagella operate in the same way?
November 10, 2025 at 5:45 PM
Reposted by (GIRISH)MALI LAB
alanbrownhms.bsky.social
Our latest collaborative effort - with Eva Gluenz and @zephyris-science.bsky.social - to understand how the various proteins and complexes of the axoneme contribute to ciliary motility:
matthdoran.bsky.social Matt Doran @matthdoran.bsky.social · 15d
In an eight-person rowing boat, each rower contributes to movement but also has a unique role: balancing, powering, or setting the rhythm and pace. In our latest collaborative work we asked – do the eight dynein “rowers” in #cilia and #flagella operate in the same way?
November 10, 2025 at 7:58 PM
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npr.org
The construction industry, where more than a quarter of workers are foreign-born, has long struggled to find enough workers. Now, industry officials say Trump's immigration crackdown is making it worse.
ICE is sending a chill through the construction industry
The construction industry, where more than a quarter of workers are foreign-born, has long struggled to find enough workers. Now, industry officials say Trump's immigration crackdown is making it worse.
n.pr
November 11, 2025 at 3:52 AM
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jcellsci.bsky.social
Also in Issue 20:
- Editorial from Pleasantine Mill @cilialab.bsky.social and Lotte Pedersen @lottepedersen.bsky.social.
- 'Cell Scientist to Watch' interviews with Keren Hilgendorf and Girish Ram Mali @dyneinassembly.bsky.social
journals.biologists.com/jcs/issue/13...
#JCSciliaSI
Keren Hilgendorf Girish Ram Mali
November 4, 2025 at 1:36 PM
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lottepedersen.bsky.social
It was a great pleasure working with @cilialab.bsky.social and @seemagrewal.bsky.social plus others at @jcellsci.bsky.social on this special issue on ‘Cilia and flagella’. Lots of cool stuff on #cilia #flagella in there, enjoy!
November 4, 2025 at 9:17 AM
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rburdine1.bsky.social
We are thrilled to see this out in @jcellsci.bsky.social an in the special issue on Cilia. Cfap298 - which we call Kurly - is the mutant that keeps on giving us surprises! In this paper, a mutation generated by CRISPR, that deletes two aa and changes a third, specifically affects cilia motility.
jcellsci.bsky.social Journal of Cell Science @jcellsci.bsky.social · 22d
Marvin Cortez, Cullen Young, Rebecca Burdine @rburdine1.bsky.social and colleagues identify a conserved domain in Cfap298 that governs left–right symmetry breaking in vertebrates.
#JCSciliaSI
journals.biologists.com/jcs/article/...
November 3, 2025 at 6:33 PM
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seemagrewal.bsky.social
Our special issue on ‘Cilia and Flagella’ is now out! Huge thanks to all (authors + reviewers) who contributed, and to our wonderful Guest Editors (@cilialab.bsky.social + @lottepedersen.bsky.social) for pulling it together.
Happy reading!
#cilia #flagella

journals.biologists.com/jcs/issue/13...
Volume 138 Issue 20 | Journal of Cell Science | The Company of Biologists
Journal of Cell Science publishes cutting-edge science encompassing all aspects of cell biology. It is published by The Company of Biologists, a not-for-profit organisation.
journals.biologists.com
November 3, 2025 at 5:32 PM
dyneinassembly.bsky.social
This was a fun chat. Thanks @jcellsci.bsky.social for featuring me and spotlighting our work.

Check out the special issue on Cilia and Flagella journals.biologists.com/jcs/issue/13....

We have an article on Axonemal Dynein Assembly Factors (DNAAFs) which may be useful for the field.
October 31, 2025 at 8:44 AM
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sjorsscheres.bsky.social
We are looking for a computational postdoc to work with us on new optimisation algorithms to make #RELION even better. Join our bubbly team at the @mrclmb.bsky.social in Cambridge, UK. 🤗 RTs appreciated.

mrc.tal.net/vx/appcentre...
Quick Check Needed
mrc.tal.net
October 29, 2025 at 9:17 AM
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cellclub.bsky.social
Structural dissection of αβ-tubulin heterodimer assembly and disassembly by human tubulin–specific chaperones | Science www.science.org/doi/10.1126/...
Structural dissection of αβ-tubulin heterodimer assembly and disassembly by human tubulin–specific chaperones
Microtubule assembly requires a set of chaperones known as tubulin-binding cofactors (TBCs). We used cryo–electron microscopy to visualize how human TBCD, TBCE, TBCC, and guanosine triphosphatase (GTP...
www.science.org
October 30, 2025 at 8:38 PM