Johannes Hohlbein
@hohlbeinlab.bsky.social
Single-molecule stuff, 🔬, 🦠 , 🧬, ☕, Assoc. Prof. @WUR (Laboratory of Biophysics, Wageningen), he/him, 🇩🇪->🇨🇿->🇩🇪->🇬🇧->🇳🇱, Pubs: https://scholar.google.nl/citations?hl=en&user=WZZAydwAAAAJ&view_op=list_works&sortby=pubdate
Pinned
First attempt in a (loosely defined) Single-Molecule Biophysics starter pack. Please share and (self-) nominate. go.bsky.app/CteFZM5
Reposted by Johannes Hohlbein
I have created an app to help students learn about emission and absorption spectra. Here is the link. I hope you find it useful 🌟
#iTeachPhysics
#Science 🧪
#Astronomy 🔭
sites.google.com/view/hookean...
#iTeachPhysics
#Science 🧪
#Astronomy 🔭
sites.google.com/view/hookean...
February 2, 2026 at 1:35 PM
I have created an app to help students learn about emission and absorption spectra. Here is the link. I hope you find it useful 🌟
#iTeachPhysics
#Science 🧪
#Astronomy 🔭
sites.google.com/view/hookean...
#iTeachPhysics
#Science 🧪
#Astronomy 🔭
sites.google.com/view/hookean...
Reposted by Johannes Hohlbein
iCASE PhD project available in my lab, combining microscopy method development with high throughput screening of bacteria. Applications to antibiotic drug discovery and screening for new cell division proteins. Collaboration with Cairn Research #microsky #bioimaging
www.findaphd.com/phds/project...
www.findaphd.com/phds/project...
Image-based bacterial phenotyping and antimicrobial drug discovery using smart high-throughput super-resolution microscopy at University of Warwick on FindAPhD.com
PhD Project - Image-based bacterial phenotyping and antimicrobial drug discovery using smart high-throughput super-resolution microscopy at University of Warwick, listed on FindAPhD.com
www.findaphd.com
February 2, 2026 at 11:11 AM
iCASE PhD project available in my lab, combining microscopy method development with high throughput screening of bacteria. Applications to antibiotic drug discovery and screening for new cell division proteins. Collaboration with Cairn Research #microsky #bioimaging
www.findaphd.com/phds/project...
www.findaphd.com/phds/project...
Reposted by Johannes Hohlbein
Great to see our Science Review on emerging multiplexed single-molecule methods that use libraries to link sequence to dynamics. A fun collaborative writing project! From sequence to function: Bridging single-molecule kinetics and molecular diversity | Science www.science.org/doi/10.1126/...
From sequence to function: Bridging single-molecule kinetics and molecular diversity
Biological function is fundamentally determined by nucleic acid and protein sequence. Beyond encoding genetic information, nucleic acids also display complex physicochemical parameters that shape stru...
www.science.org
January 30, 2026 at 6:59 PM
Great to see our Science Review on emerging multiplexed single-molecule methods that use libraries to link sequence to dynamics. A fun collaborative writing project! From sequence to function: Bridging single-molecule kinetics and molecular diversity | Science www.science.org/doi/10.1126/...
The Laboratory of Biophysics at Wageningen University & Research (NL) is looking for a new chair! Details here: www.wur.nl/en/vacancy/f...
Full Professor and Chair Laboratory of Biophysics
We are looking for a Full Professor in the Biophysics chair group, part of Wageningen University & Research (WUR)
www.wur.nl
January 27, 2026 at 8:13 PM
The Laboratory of Biophysics at Wageningen University & Research (NL) is looking for a new chair! Details here: www.wur.nl/en/vacancy/f...
Good resource for everyone interested in objectives and microscopy 👇
Yes I wish the FOV on Nikon (and other) objectives was better specified. Some models are labelled OFN22, others OFN25. The new TIRF series is supposedly compatible with the new Ti2 platform (25mm FOV) but I can't find a definitive spec.
More thoughts here:
amsikking.github.io/microscope_o...
More thoughts here:
amsikking.github.io/microscope_o...
amsikking: Microscope objectives
amsikking.github.io
January 26, 2026 at 7:35 AM
Good resource for everyone interested in objectives and microscopy 👇
@healthcare.nikon.com or others: Where can I find information on the field number of Nikon objectives? Seems missing from the objective comparison site? Specifically wondering about the CFI Apochromat TIRF 60XC Oil.
January 25, 2026 at 1:54 PM
@healthcare.nikon.com or others: Where can I find information on the field number of Nikon objectives? Seems missing from the objective comparison site? Specifically wondering about the CFI Apochromat TIRF 60XC Oil.
First rule when receiving a unsolicited chain mail at uni: REPLY ALL and say that you want to be removed from the list...
January 19, 2026 at 10:51 AM
First rule when receiving a unsolicited chain mail at uni: REPLY ALL and say that you want to be removed from the list...
Reposted by Johannes Hohlbein
I finally got around to writing the discussion section of our lab's basic training course on live cell #microscopy. I explain the tradeoffs involved in designing an imaging experiment and the art of thinking of the experiment as an optimization problem.
leb-epfl.github.io/basic_traini...
leb-epfl.github.io/basic_traini...
Introduction - Microscopy Basic Training
leb-epfl.github.io
January 13, 2026 at 3:07 PM
I finally got around to writing the discussion section of our lab's basic training course on live cell #microscopy. I explain the tradeoffs involved in designing an imaging experiment and the art of thinking of the experiment as an optimization problem.
leb-epfl.github.io/basic_traini...
leb-epfl.github.io/basic_traini...
Reposted by Johannes Hohlbein
I guess I should re-post from the Bird Site "The Rules For Academic Success here":
I always get asked for advice about starting/surviving the tenure track. Here are the rules I've found useful. Thought I'd put it out there for the masses...
This list gets longer all the time
I always get asked for advice about starting/surviving the tenure track. Here are the rules I've found useful. Thought I'd put it out there for the masses...
This list gets longer all the time
August 1, 2023 at 6:30 PM
I guess I should re-post from the Bird Site "The Rules For Academic Success here":
I always get asked for advice about starting/surviving the tenure track. Here are the rules I've found useful. Thought I'd put it out there for the masses...
This list gets longer all the time
I always get asked for advice about starting/surviving the tenure track. Here are the rules I've found useful. Thought I'd put it out there for the masses...
This list gets longer all the time
TIL: 5 cm snow all over the Netherlands equates to around 300 billion kg of snow. nos.nl/liveblog/259...
Drukste woensdagochtendspits in jaren • NS roept mensen op treinreis uit te stellen
In dit liveblog houden we je op de hoogte van het aanhoudende winterse weer in Nederland.
nos.nl
January 7, 2026 at 8:50 AM
TIL: 5 cm snow all over the Netherlands equates to around 300 billion kg of snow. nos.nl/liveblog/259...
Reposted by Johannes Hohlbein
A new year brings new opportunities: we are looking to fill a permanent professorship position with a Microbiologist preferably with expertise on archaeal biology, RNA biology or imaging techniques to unravel cell biology in prokaryotes! Please share and/or apply :)! www.nature.com/naturecareer...
Professurship (W2) in Microbiology - Regensburg (Stadt), Bayern (DE) job with Universität Regensburg | 12851113
UNIVERSITY OF REGENSBURG The Faculty of Biology and Pre-Clinical Medicine invites applications for a Professurship (W2) in Microbiology To be...
www.nature.com
January 5, 2026 at 9:46 AM
A new year brings new opportunities: we are looking to fill a permanent professorship position with a Microbiologist preferably with expertise on archaeal biology, RNA biology or imaging techniques to unravel cell biology in prokaryotes! Please share and/or apply :)! www.nature.com/naturecareer...
Reposted by Johannes Hohlbein
2025 was the year we stopped debating whether paying peer reviewers might work and started showing that it does.
December 31, 2025 at 10:13 PM
2025 was the year we stopped debating whether paying peer reviewers might work and started showing that it does.
I vaguely remember seeing a discussion about lab-onboarding etc on Twitter many years ago. This initiative below and the handbook look really valuable. Will implement.
A new community-driven lab handbook for reducing conflict and creating more positive and equitable work environments gets strong support from a survey of 200 researchers.
buff.ly/K7CGFLV
buff.ly/K7CGFLV
January 2, 2026 at 12:36 PM
I vaguely remember seeing a discussion about lab-onboarding etc on Twitter many years ago. This initiative below and the handbook look really valuable. Will implement.
“Coffee doesn't talk, coffee doesn't whine, coffee just does its job. I like coffee.” Happy new year!
January 1, 2026 at 9:36 AM
“Coffee doesn't talk, coffee doesn't whine, coffee just does its job. I like coffee.” Happy new year!
Perspective is everything in life.
December 24, 2025 at 10:42 PM
Perspective is everything in life.
Remarkable. I have many questions.
December 22, 2025 at 6:49 PM
Remarkable. I have many questions.
Reposted by Johannes Hohlbein
New preprint! We share a new *DNA-only* method for single-molecule DNA sequencing, and use this cool tool to link single-molecule reactions/interactions to sequence. Big thanks🙏 to Jagadish Hazra @becs-andrews.bsky.social and all contributors since our 2016 start!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Unraveling single-molecule reactions via multiplexed in-situ DNA sequencing
DNA sequence regulates complex reactions and protein-DNA interactions, yet sequence effects remain poorly understood due to the lack of direct, high-throughput approaches to study sequence-dependence ...
www.biorxiv.org
December 5, 2025 at 12:04 PM
New preprint! We share a new *DNA-only* method for single-molecule DNA sequencing, and use this cool tool to link single-molecule reactions/interactions to sequence. Big thanks🙏 to Jagadish Hazra @becs-andrews.bsky.social and all contributors since our 2016 start!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
The future has arrived.
December 4, 2025 at 4:52 PM
The future has arrived.
Reposted by Johannes Hohlbein
I am very excited to announce that a fully funded PhD position is available in my group.
Topic: Synergistic coevolution in mono-specific and multi-species microbial consortia
Please RT or forward this information to interested candidates.
Deadline: 11.01.26
More info:
shorturl.at/f1TuF
Topic: Synergistic coevolution in mono-specific and multi-species microbial consortia
Please RT or forward this information to interested candidates.
Deadline: 11.01.26
More info:
shorturl.at/f1TuF
November 27, 2025 at 2:24 PM
I am very excited to announce that a fully funded PhD position is available in my group.
Topic: Synergistic coevolution in mono-specific and multi-species microbial consortia
Please RT or forward this information to interested candidates.
Deadline: 11.01.26
More info:
shorturl.at/f1TuF
Topic: Synergistic coevolution in mono-specific and multi-species microbial consortia
Please RT or forward this information to interested candidates.
Deadline: 11.01.26
More info:
shorturl.at/f1TuF
Reposted by Johannes Hohlbein
We are looking for a new technician 🥼 - initially limited with the option to a permanent position. If you are interested in molecular biology🧫, biochemistry🧬, a dynamic🕺, international🌍, welcoming science enthusiasts 🧑🔬 take a closer look👇 - Re-post appreciated www.verw.tu-dresden.de/StellAus/ste...
Vacancy ID 12501
Am Center for Molecular Bioengineering (B CUBE), einem Institut des Center for Molecular and Cellular Bioengineering (CMCB), ist an der Professur für Molekulare Biophysik ab sofort eine Stelle als
www.verw.tu-dresden.de
November 21, 2025 at 2:12 PM
We are looking for a new technician 🥼 - initially limited with the option to a permanent position. If you are interested in molecular biology🧫, biochemistry🧬, a dynamic🕺, international🌍, welcoming science enthusiasts 🧑🔬 take a closer look👇 - Re-post appreciated www.verw.tu-dresden.de/StellAus/ste...
Wait?! There are scientific 532nm and 561 nm diode lasers available these days? Direct analogue and digital modulation?! 🤯
November 21, 2025 at 2:59 PM
Wait?! There are scientific 532nm and 561 nm diode lasers available these days? Direct analogue and digital modulation?! 🤯
Friend of mine recently vibe-coded a small webapp for the Eisenhower principle (four-step prioritization tool). Code is open, feedback is welcome: s540d.github.io/Eisenhauer/
November 21, 2025 at 12:45 PM
Friend of mine recently vibe-coded a small webapp for the Eisenhower principle (four-step prioritization tool). Code is open, feedback is welcome: s540d.github.io/Eisenhauer/
Reposted by Johannes Hohlbein
We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt
www.nature.com/articles/s41...
rdcu.be/eQVxt
4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods
4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.
www.nature.com
November 21, 2025 at 3:00 AM
We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt
www.nature.com/articles/s41...
rdcu.be/eQVxt
Reposted by Johannes Hohlbein
We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.
It ships many new features, detailed below, but that are articulated around the following:
It ships many new features, detailed below, but that are articulated around the following:
November 20, 2025 at 11:42 AM
We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.
It ships many new features, detailed below, but that are articulated around the following:
It ships many new features, detailed below, but that are articulated around the following: