Michaela Novotná
@michaelanovotna.bsky.social
130 followers 290 following 5 posts
PhD student in @balikovalab.bsky.social at @czechacademy.bsky.social of Sciences, BIOCEV, @charlesuni.cuni.cz in Prague 💊 antibiotics 💎 ribosome 🧫 resistance 🧬 ABC-Fs 🦠 staph ❄️ cryo-em @mbuavcr.bsky.social @sciencecharles.bsky.social
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Reposted by Michaela Novotná
nivbcz.bsky.social
Dvě vědkyně z @mbuavcr.bsky.social - Natalia Slonská a Lucie Najmanová, se zúčastnily soutěže Věda fotogenická, kterou pořádá @akademievedcr.bsky.social. Do soutěže přihlásily snímky, které propojují krásu květu fuchsie se světem půdních bakterií Streptomyces coelicolor.
Reposted by Michaela Novotná
Reposted by Michaela Novotná
olibclarke.bsky.social
This one is a bit of a departure from the usual and definitely a work in progress!

We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.

Read on for details... 1/x
Reposted by Michaela Novotná
Check out our new paper: a review of translational coupling, the phenomenon where translation of one prokaryotic gene can promote translation of the gene downstream. We cover the history, and delve into the mechanism, which is still not fully understood. journals.asm.org/doi/10.1128/...
Translational coupling of neighboring genes in prokaryotes | Journal of Bacteriology
Prokaryotic genes are arranged in operons, with functionally related genes often located adjacent to one another (1). There are several ways in which the operonic organization of genes facilitates the...
journals.asm.org
Reposted by Michaela Novotná
biorxiv-biochem.bsky.social
Structural insights into YheS-mediated release of SecM-arrested ribosome. https://www.biorxiv.org/content/10.1101/2025.08.28.672766v1
Reposted by Michaela Novotná
raphael-microb.bsky.social
OMG this is it?! The final piece in the puzzle?!😲 tRNAs are the key, because archaic tRNAs sit at the core of all ribosomes. If aminoacyl-tRNAs could form on early Earth, you have a plausible route for the first self-replicators, the bridge from chemistry to biology
www.nature.com/articles/d41...
Origins of life: the molecules that could have unlocked peptide synthesis
For life to emerge on Earth, peptides must first have formed without the aid of enzymes — but how? Reactions of sulfur-containing molecules might have been key.
www.nature.com
Reposted by Michaela Novotná
michaelanovotna.bsky.social
Happy to share a new paper from our lab with my little contribution! 🥳🥳🥳
We show that antibiotic resistance ABC-F proteins fine-tune responses to ribosome-binding antibiotics. Thanks to ABC-Fs, bacteria can differentiate between antibiotic classes and even concentrations for a tailored response. 🦠💊⬇️
Reposted by Michaela Novotná
jbquerido.bsky.social
Finally out!
I’m thrilled to share our new paper (Wolin et al., Cell 2025).

This paper describes SPIDR, a high-throughput method for mapping RBP binding sites.

By combining #SPIDR with #cryoEM, we identified the exact binding site of LARP1 within the #mRNA channel of the 40S ribosomal subunit.
Reposted by Michaela Novotná
thebanlab.bsky.social
New paper out in Mol Cell, where we show how N-myristolytransferases lipidate proteins on the translating ribosome!
Continuing our successful collaboration with DeuerlingLab @uni-konstanz.de & Shu-ou Chan @caltech.edu
@snsf.ch @ethz.ch
www.cell.com/molecular-ce...
Reposted by Michaela Novotná
cryoempapers.bsky.social
Structural studies on ribosomes of differentially macrolide-resistant Staphylococcus aureus strains pubmed.ncbi.nlm.nih.gov/40490363/ #cryoem
Reposted by Michaela Novotná
cryoempapers.bsky.social
In situ protein crystallography: a single-crystal electron diffraction pipeline for structure determination inside living cells. www.biorxiv.org/content/10.1101/2025.07.08.663504v1 #cryoem
Reposted by Michaela Novotná
zeynepbaharoglu.bsky.social
Insights on how ribosomal RNA modification distant from drug binding site can still affect drug binding, in mycobacterium 🦠 #rnasky #ribosome #microsky
cryoempapers.bsky.social
Distant ribose 2'-O-methylation of 23S rRNA helix 69 pre-orders the capreomycin drug binding pocket at the ribosome subunit interface pubmed.ncbi.nlm.nih.gov/40626557/ #cryoem
Reposted by Michaela Novotná
dijiang319.bsky.social
@science.org Mechanism of release factor–mediated peptidyl-tRNA hydrolysis on the ribosome | Science www.science.org/doi/10.1126/...
#ribosome #RNA #hydrolysis
Reposted by Michaela Novotná
merveyigit.bsky.social
I’m thrilled to share my very first first-author preprint and our lab’s latest work💫 We discovered a novel role for eIF2A in ribosome-associated quality control (RQC), challenging decades-old assumptions about its enigmatic function in translation initiation.

Check it out to learn more! ⭐️
#RNA
Reposted by Michaela Novotná
wilsonlab.bsky.social
In our newly published study we show that the proline-rich antimicrobial peptide (PrAMP) Bac5 has a distinct mode of interaction with the ribosome compared to other PrAMPs…https://academic.oup.com/nar/article/53/8/gkaf324/8126264
The structure of the Vibrio natriegens 70S ribosome in complex with the proline-rich antimicrobial peptide Bac5(1–17)
Abstract. Proline-rich antimicrobial peptides (PrAMPs) are produced as part of the innate immune response of animals, insects, and plants. The well-charact
academic.oup.com