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Reposted by Michael Grange

Lori Passmore @lapassmore.bsky.social · 12d

We have an open post-doc position in my group to study mRNA cleavage and polyadenylation using biochemical reconstitution and cryoEM.

Please get in touch if you are interested in joining this amazing team! 🔬🧬🤩
#RNA #cryoEM

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Reposted by Michael Grange

Ben Engel @cellarchlab.com · 14d

Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on!

#TeamTomo #PlantScience 🧪 🧶🧬 🌾
elifesciences.org/articles/105...
1/🧵

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Reposted by Michael Grange

Guenter Resch @nexperion.net · 16d

Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit
www.nexperion.net/semcm2026

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Reposted by Michael Grange

Kat Toropova @kattoropova.bsky.social · Sep 8

The Dunn School PhD programme is live! 🎉

Two projects in our labs:
1) How the motor protein giant dynein-2 works
2) How signalling-essential membrane proteins get into cilia

Cryo-EM/ET, single mol & live-cell fluor microscopy, CRISPR-based genetic tools & more!

www.path.ox.ac.uk/study-with-u...

How to Apply - Dunn School

Here you can find more information on how to apply for our PhD programme, including deadlines and application requirements.

www.path.ox.ac.uk

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Reposted by Michael Grange

Pombo-GarciaLab @pombo-garcialab.bsky.social · Sep 6

🚨 We are hiring a #postdoc in Quantitative Cell Biology/Biochemistry. Join us if you are interested in using super-resolution STED & cryoET to uncover self-organization dynamics of epithelial cell interfaces & glycobiology collaboration with WuLab
zurl.co/aTGq7

About The Rosalind Franklin Institute - Rosalind Franklin Institute

The Rosalind Franklin Institute is a new national institute, announced in 2017 by the UK government, dedicated to bringing about transformative changes in life science through interdisciplinary resear...

zurl.co

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Reposted by Michael Grange

Tanmay Bharat @tbharat-lab.bsky.social · Aug 31

Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging

Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social

www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social

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Reposted by Michael Grange

Jenny Sachweh @jennysachweh.bsky.social · Aug 18

My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...!

Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!

Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo

The small GTPase Ran defines nuclear pore complex asymmetry

Nuclear pore complexes (NPCs) bridge across the nuclear envelope and mediate nucleocytoplasmic exchange. They consist of hundreds of nucleoporin build…

www.sciencedirect.com

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Reposted by Michael Grange

Chi-Min Ho @cmholab.bsky.social · Aug 18

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH

A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug

Nature Structural & Molecular Biology - Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation...

rdcu.be

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Reposted by Michael Grange

Sjors Scheres @sjorsscheres.bsky.social · Aug 5

Fantastic work by the group of @matteoall.bsky.social : a SUN5:Nesprin3 complex at the head:tail junction of sperm cells. 🤯 #teamtomo

www.biorxiv.org/content/10.1...

In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells

In eukaryotic cells, LINC complexes physically bridge the two nuclear membranes to cytoskeletal filaments, transmitting mechanical forces to the nucleus. Due to their dynamic and membrane-embedded nat...

www.biorxiv.org

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Reposted by Michael Grange

Marcel Dickmanns | 🌿❄️🔬 @madic.bsky.social · Jul 26

New preprint: In situ Architecture of #Plasmodesmata.

Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores.

🔗 doi.org/10.1101/2025...

🧪🧵1/n
#teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM

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Michael Grange @michaelgrange.bsky.social · Jul 21

And here it is now in print! www.cell.com/cell-reports...

With a front cover splash 😍

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Reposted by Michael Grange

Thom Sharp @thomsharp.bsky.social · Jul 9

We’re hiring a postdoc! 🔬

Join us in Bristol, UK to study in situ structural immunology using cryoFIB-SEM + cryoET on our new Hydra Bio system. ERC-funded, 3 years, studying phagocytosis to develop new immunotherapeutics.

Closing 29 July 2025.

Info + apply: www.bristol.ac.uk/jobs/find/de...

Details | Working at Bristol | University of Bristol

www.bristol.ac.uk

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Michael Grange @michaelgrange.bsky.social · Jul 2

Congrats Martin!

Michael Grange @michaelgrange.bsky.social · Jun 20

Thanks Ben. Looking forward to see how much added help v2 can bring!

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Reposted by Michael Grange

Paul & Virginie - Centriole Lab @centriolelab.bsky.social · Jun 19

🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...

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Reposted by Michael Grange

cryoEM papers @cryoempapers.bsky.social · Jun 18

A generalizable and targeted molecular biopsy approach for in situ cryogenic electron tomography of vitreous brain tissue pubmed.ncbi.nlm.nih.gov/40527314/ #cryoem

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Reposted by Michael Grange

fitzpatricklab.bsky.social @fitzpatricklab.bsky.social · Jun 17

New preprint from Daniel Du @fitzpatricklab.bsky.social shows #femtosecond light pulses can lens electron beams, promising higher contrast in #cryoET and better aberration correction in #cryoEM

tinyurl.com/257sw7j9

Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture

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Michael Grange @michaelgrange.bsky.social · Jun 17

So, what's next?

In the lab, we are already applying this workflow to different systems and tissues reproducibly.

Whatever cell biology questions drive your research, we hope you can implement our approach to gain molecular level insights within the native tissue environment.

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Michael Grange @michaelgrange.bsky.social · Jun 17

finally, using plasma allows you to reel in WHOPPERS you can see from space!

@cryingem.bsky.social 🎣🎣🎣🐟🐟🐟

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Michael Grange @michaelgrange.bsky.social · Jun 17

Geometry was key - FIBs are flexible!

Having the ability to image the dendritic network from alternate perspectives using planar lift out gives us different insights.

In this case, molecular variabilities in the microtubule network could be teased out.

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Michael Grange @michaelgrange.bsky.social · Jun 17

Assessing hundreds of sections, they optimised targeting, producing numerous snapshots of synaptic organisation in tissues.

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Michael Grange @michaelgrange.bsky.social · Jun 17

Obviously, if you want native proteins the sample needs to be vitreous.

It was a heroic effort to establish proper vitreous cryo-fixation, testing many cryoprotectants - lifting out and imaging non vitreous samples is not fun!

Here's our blooper reel.

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Michael Grange @michaelgrange.bsky.social · Jun 17

Proud to share our latest paper. doi.org/10.1016/j.cr...

Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.

Structural biology in tissue is well and truly here!

@rosfrankinst.bsky.social

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Michael Grange @michaelgrange.bsky.social · Jun 10

Sorry Elizabeth, you (and the rest of the US scientific community) deserve better.

Michael Grange @michaelgrange.bsky.social · May 17

They do look kinda cute in the process tho…

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