@fitzpatricklab.bsky.social
Pinned
New preprint from Daniel Du @fitzpatricklab.bsky.social shows #femtosecond light pulses can lens electron beams, promising higher contrast in #cryoET and better aberration correction in #cryoEM
tinyurl.com/257sw7j9
Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture
tinyurl.com/257sw7j9
Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture
Reposted
🚀 CryoSPARC v5.0 BETA is here!
We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread!
Full changelog: cryosparc.com/updates/v5.0.0
We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread!
Full changelog: cryosparc.com/updates/v5.0.0
January 27, 2026 at 8:36 PM
🚀 CryoSPARC v5.0 BETA is here!
We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread!
Full changelog: cryosparc.com/updates/v5.0.0
We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread!
Full changelog: cryosparc.com/updates/v5.0.0
Frozen Bryant Park fountain in snowy NYC!
January 28, 2026 at 12:15 AM
Frozen Bryant Park fountain in snowy NYC!
Reposted
A combined approach using light microscopy and ion beam milling enhances the precision of cryoET sample preparation, enabling more accurate targeting of small, rare structures within cells. doi.org/hbkb94
Tightening the focus of subcellular snapshots: Combined approach yields better cell slices for cryoET imaging
Taking images of tiny structures within cells is tricky business. One technique, cryogenic electron tomography (cryoET), shoots electrons through a frozen sample.
phys.org
January 17, 2026 at 8:20 PM
A combined approach using light microscopy and ion beam milling enhances the precision of cryoET sample preparation, enabling more accurate targeting of small, rare structures within cells. doi.org/hbkb94
Reposted
Reversible lipid-mediated pH-gating of connexin-46/50 by cryo-EM pubmed.ncbi.nlm.nih.gov/41526355/ #cryoem
January 13, 2026 at 6:47 PM
Reversible lipid-mediated pH-gating of connexin-46/50 by cryo-EM pubmed.ncbi.nlm.nih.gov/41526355/ #cryoem
Reposted
Amyloid-mediated RNA uptake by sperm for embryonic delivery https://www.biorxiv.org/content/10.64898/2025.12.10.693493v1
December 12, 2025 at 4:47 AM
Amyloid-mediated RNA uptake by sperm for embryonic delivery https://www.biorxiv.org/content/10.64898/2025.12.10.693493v1
Reposted
Excited to share our latest research, out today in Cell (@cellpress.bsky.social)
Using cryo-EM and cell-based studies, we reveal how large nutrient sensing protein complexes form at the lysosomal membrane to turn off mTORC1 and halt anabolic signalling.
Read the paper here: doi.org/10.1016/j.ce...
Using cryo-EM and cell-based studies, we reveal how large nutrient sensing protein complexes form at the lysosomal membrane to turn off mTORC1 and halt anabolic signalling.
Read the paper here: doi.org/10.1016/j.ce...
January 9, 2026 at 7:11 AM
Excited to share our latest research, out today in Cell (@cellpress.bsky.social)
Using cryo-EM and cell-based studies, we reveal how large nutrient sensing protein complexes form at the lysosomal membrane to turn off mTORC1 and halt anabolic signalling.
Read the paper here: doi.org/10.1016/j.ce...
Using cryo-EM and cell-based studies, we reveal how large nutrient sensing protein complexes form at the lysosomal membrane to turn off mTORC1 and halt anabolic signalling.
Read the paper here: doi.org/10.1016/j.ce...
Another member of the Fitzpatrick/Shapiro quad @zuckermanbrain.bsky.social embarks on setting up his lab!
If you wish to use #cryoEM #cryoET to study how viruses enter & evade the immune system, & design antibody-based vaccines, check out the Roark lab:
isb.med.upenn.edu/research/cryoem-and-cryoet
If you wish to use #cryoEM #cryoET to study how viruses enter & evade the immune system, & design antibody-based vaccines, check out the Roark lab:
isb.med.upenn.edu/research/cryoem-and-cryoet
January 2, 2026 at 10:55 PM
Another member of the Fitzpatrick/Shapiro quad @zuckermanbrain.bsky.social embarks on setting up his lab!
If you wish to use #cryoEM #cryoET to study how viruses enter & evade the immune system, & design antibody-based vaccines, check out the Roark lab:
isb.med.upenn.edu/research/cryoem-and-cryoet
If you wish to use #cryoEM #cryoET to study how viruses enter & evade the immune system, & design antibody-based vaccines, check out the Roark lab:
isb.med.upenn.edu/research/cryoem-and-cryoet
Reposted
Organelles do NOT have a single uniform pH.
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
Organelles harbour pH gradients https://www.biorxiv.org/content/10.64898/2025.12.12.694065v1
December 17, 2025 at 12:46 AM
Organelles do NOT have a single uniform pH.
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
Reposted
Thrilled to see our review article "The evolutionary origins of synaptic proteins" highlighted on the cover of Nature Reviews Neuroscience 🤩.
www.nature.com/articles/s41...
@msarscentre.bsky.social 🧠✨🧬🌊🪼🧽
www.nature.com/articles/s41...
@msarscentre.bsky.social 🧠✨🧬🌊🪼🧽
December 17, 2025 at 3:25 PM
Thrilled to see our review article "The evolutionary origins of synaptic proteins" highlighted on the cover of Nature Reviews Neuroscience 🤩.
www.nature.com/articles/s41...
@msarscentre.bsky.social 🧠✨🧬🌊🪼🧽
www.nature.com/articles/s41...
@msarscentre.bsky.social 🧠✨🧬🌊🪼🧽
Reposted
A quick tutorial on the DAQ structure validation score for protein models derived from cryo-EM. DAQ evaluates amino acid–level accuracy in your model. Consider including a DAQ validation report in your next publication!
www.youtube.com/watch?v=YRAT...
www.youtube.com/watch?v=YRAT...
DAQ-Score: Automatic AI-based Cryo-EM Structure Model Validation!
YouTube video by Kihara Bioinformatics Lab
www.youtube.com
December 14, 2025 at 8:44 PM
A quick tutorial on the DAQ structure validation score for protein models derived from cryo-EM. DAQ evaluates amino acid–level accuracy in your model. Consider including a DAQ validation report in your next publication!
www.youtube.com/watch?v=YRAT...
www.youtube.com/watch?v=YRAT...
Reposted
I’m very happy (and grateful!) to share that I’m featured in a Nature Cancer Viewpoint about starting my lab at CNIO.
In the Lab (www.rcglab.com) we’re combining AI, protein design and structural biology to study protein structures and design novel protein therapeutics for cancer. 🔬
In the Lab (www.rcglab.com) we’re combining AI, protein design and structural biology to study protein structures and design novel protein therapeutics for cancer. 🔬
December 14, 2025 at 6:31 PM
I’m very happy (and grateful!) to share that I’m featured in a Nature Cancer Viewpoint about starting my lab at CNIO.
In the Lab (www.rcglab.com) we’re combining AI, protein design and structural biology to study protein structures and design novel protein therapeutics for cancer. 🔬
In the Lab (www.rcglab.com) we’re combining AI, protein design and structural biology to study protein structures and design novel protein therapeutics for cancer. 🔬
Reposted
🔬 Just out in @NatureComms!
Using cryo-CLEM and cryo-ET, we reveal the unique ultrastructure of dopaminergic varicosities in the striatum.
Amazing collaboration with @davip-bdx.bsky.social and Etienne Herzog @iins-bordeaux.bsky.social
Congratulations to Paul and Robin for this fantastic work! 🎉
Using cryo-CLEM and cryo-ET, we reveal the unique ultrastructure of dopaminergic varicosities in the striatum.
Amazing collaboration with @davip-bdx.bsky.social and Etienne Herzog @iins-bordeaux.bsky.social
Congratulations to Paul and Robin for this fantastic work! 🎉
Cryo-correlative light and electron tomography of dopaminergic axonal varicosities reveals non-synaptic modulation of cortico-striatal synapses - Nature Communications
The basic features of dopamine release sites are still largely unknown. Here, the authors determine the ultrastructure of fluorescent dopaminergic and glutamatergic synaptosomes in mouse striatum usin...
www.nature.com
December 13, 2025 at 8:11 PM
🔬 Just out in @NatureComms!
Using cryo-CLEM and cryo-ET, we reveal the unique ultrastructure of dopaminergic varicosities in the striatum.
Amazing collaboration with @davip-bdx.bsky.social and Etienne Herzog @iins-bordeaux.bsky.social
Congratulations to Paul and Robin for this fantastic work! 🎉
Using cryo-CLEM and cryo-ET, we reveal the unique ultrastructure of dopaminergic varicosities in the striatum.
Amazing collaboration with @davip-bdx.bsky.social and Etienne Herzog @iins-bordeaux.bsky.social
Congratulations to Paul and Robin for this fantastic work! 🎉
Reposted
December Update of DAQ-Score DB! Quality evaluations of protein models from cryo-EM. Now includes evaluation of 258,956 protein chains.
Check at daqdb.kiharalab.org
It's very easy to compute DAQ for your protein model. Add the validation result in your publication: em.kiharalab.org/algorithm/da...
Check at daqdb.kiharalab.org
It's very easy to compute DAQ for your protein model. Add the validation result in your publication: em.kiharalab.org/algorithm/da...
December 13, 2025 at 2:15 PM
December Update of DAQ-Score DB! Quality evaluations of protein models from cryo-EM. Now includes evaluation of 258,956 protein chains.
Check at daqdb.kiharalab.org
It's very easy to compute DAQ for your protein model. Add the validation result in your publication: em.kiharalab.org/algorithm/da...
Check at daqdb.kiharalab.org
It's very easy to compute DAQ for your protein model. Add the validation result in your publication: em.kiharalab.org/algorithm/da...
Reposted
Holy cow the Zhou lab at UCLA is cooking with Isonet 2 - www.biorxiv.org/content/10.6...
Averaging-free direct visualization of ribosome translational state! #teamtomo #cryoet
Averaging-free direct visualization of ribosome translational state! #teamtomo #cryoet
December 12, 2025 at 3:53 PM
Holy cow the Zhou lab at UCLA is cooking with Isonet 2 - www.biorxiv.org/content/10.6...
Averaging-free direct visualization of ribosome translational state! #teamtomo #cryoet
Averaging-free direct visualization of ribosome translational state! #teamtomo #cryoet
Reposted
IsoNet2 determines cellular structures at submolecular resolution without averaging https://www.biorxiv.org/content/10.64898/2025.12.09.693325v1
December 12, 2025 at 1:55 PM
Reposted
Love the caption of Fig. 3 in this impressive preprint:
"A Goodsell-esque 3D orthogonal rendering of an IsoNet2-processed FIB-milled tomogram of C. reinhardtii"
doi.org/10.64898/202...
Since I am no expert, what does the cryoET community think of IsoNet? @cellarchlab.com
doi.org/10.1038/s414...
"A Goodsell-esque 3D orthogonal rendering of an IsoNet2-processed FIB-milled tomogram of C. reinhardtii"
doi.org/10.64898/202...
Since I am no expert, what does the cryoET community think of IsoNet? @cellarchlab.com
doi.org/10.1038/s414...
December 12, 2025 at 8:38 AM
Love the caption of Fig. 3 in this impressive preprint:
"A Goodsell-esque 3D orthogonal rendering of an IsoNet2-processed FIB-milled tomogram of C. reinhardtii"
doi.org/10.64898/202...
Since I am no expert, what does the cryoET community think of IsoNet? @cellarchlab.com
doi.org/10.1038/s414...
"A Goodsell-esque 3D orthogonal rendering of an IsoNet2-processed FIB-milled tomogram of C. reinhardtii"
doi.org/10.64898/202...
Since I am no expert, what does the cryoET community think of IsoNet? @cellarchlab.com
doi.org/10.1038/s414...
Reposted
IsoNet2 determines cellular structures at submolecular resolution without averaging www.biorxiv.org/content/10.64898/2025.12.09.693325v1 #cryoem
December 12, 2025 at 11:42 AM
IsoNet2 determines cellular structures at submolecular resolution without averaging www.biorxiv.org/content/10.64898/2025.12.09.693325v1 #cryoem
Many thanks to @cellpress.bsky.social for highlighting our #cryoEM research:
cell.com/structure/fu...
cell.com/structure/fu...
Our authors in 2025
As 2025 comes to an end, we want to highlight some of the rising young faculty members
who have published their exciting work from different areas of structural biology
in Structure this year. We have...
cell.com
December 5, 2025 at 5:07 PM
Many thanks to @cellpress.bsky.social for highlighting our #cryoEM research:
cell.com/structure/fu...
cell.com/structure/fu...
Reposted
I wrote a bit about practical considerations for using #cryo-ET labels and some things to work on if we want to find any protein in any cell.
authors.elsevier.com/a/1mA6h,LqAr...
authors.elsevier.com/a/1mA6h,LqAr...
authors.elsevier.com
December 1, 2025 at 3:43 PM
I wrote a bit about practical considerations for using #cryo-ET labels and some things to work on if we want to find any protein in any cell.
authors.elsevier.com/a/1mA6h,LqAr...
authors.elsevier.com/a/1mA6h,LqAr...
Reposted
Delighted to have contributed to this paper and amazing resource. In-silico protein-protein interaction screening has huge promise for generating novel hypotheses and building models into low-res #cryo-EM and #teamtomo maps.
Thrilled to share that the final piece of my PhD work is now on bioRxiv! biorxiv.org/content/10.1... With support from @nvidia and the @NSF, we used AlphaFold to screen 1.6M+ protein pairs, revealing thousands of potential novel PPIs. All data can be viewed at predictomes.org/hp
Proteome-wide in silico screening for human protein-protein interactions
Protein-protein interactions (PPIs) drive virtually all biological processes, yet most PPIs have not been identified and even more remain structurally unresolved. We developed a two-step computational...
biorxiv.org
November 13, 2025 at 1:27 PM
Reposted
Seeing is believing-Plasmodium falciparum translation in action pubmed.ncbi.nlm.nih.gov/41202797/ #cryoem
November 8, 2025 at 9:58 PM
Seeing is believing-Plasmodium falciparum translation in action pubmed.ncbi.nlm.nih.gov/41202797/ #cryoem
Reposted
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
November 5, 2025 at 4:23 PM
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
Reposted
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
November 4, 2025 at 5:20 PM
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
Reposted
Learn how our hunt for the native structure of top antimalarial target PfATP4🧂led to the discovery of PfABP, an unknown essential binding partner, out now! @natcomms.nature.com 👉 rdcu.be/eLRlH 🦠🔬❄️
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
Endogenous structure of antimalarial target PfATP4 reveals an apicomplexan-specific P-type ATPase modulator
Nature Communications - Here, the authors present the 3.7 Å cryoEM structure of native sodium efflux pump PfATP4 from Plasmodium falciparum, revealing a bound protein that they term...
rdcu.be
October 20, 2025 at 12:55 PM
Learn how our hunt for the native structure of top antimalarial target PfATP4🧂led to the discovery of PfABP, an unknown essential binding partner, out now! @natcomms.nature.com 👉 rdcu.be/eLRlH 🦠🔬❄️
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
Reposted
1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from?
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Antiviral reverse transcriptases reveal the evolutionary origin of telomerase
Defense-associated reverse transcriptases (DRTs) employ diverse and distinctive mechanisms of cDNA synthesis to protect bacteria against viral infection. However, much of DRT family diversity remains ...
www.biorxiv.org
October 17, 2025 at 5:22 PM
1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from?
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...