@fitzpatricklab.bsky.social
Pinned
New preprint from Daniel Du @fitzpatricklab.bsky.social shows #femtosecond light pulses can lens electron beams, promising higher contrast in #cryoET and better aberration correction in #cryoEM
tinyurl.com/257sw7j9
Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture
tinyurl.com/257sw7j9
Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture
Reposted
Delighted to have contributed to this paper and amazing resource. In-silico protein-protein interaction screening has huge promise for generating novel hypotheses and building models into low-res #cryo-EM and #teamtomo maps.
Thrilled to share that the final piece of my PhD work is now on bioRxiv! biorxiv.org/content/10.1... With support from @nvidia and the @NSF, we used AlphaFold to screen 1.6M+ protein pairs, revealing thousands of potential novel PPIs. All data can be viewed at predictomes.org/hp
Proteome-wide in silico screening for human protein-protein interactions
Protein-protein interactions (PPIs) drive virtually all biological processes, yet most PPIs have not been identified and even more remain structurally unresolved. We developed a two-step computational...
biorxiv.org
November 13, 2025 at 1:27 PM
Reposted
Seeing is believing-Plasmodium falciparum translation in action pubmed.ncbi.nlm.nih.gov/41202797/ #cryoem
November 8, 2025 at 9:58 PM
Seeing is believing-Plasmodium falciparum translation in action pubmed.ncbi.nlm.nih.gov/41202797/ #cryoem
Reposted
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
November 5, 2025 at 4:23 PM
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
Reposted
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
November 4, 2025 at 5:20 PM
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
Reposted
Learn how our hunt for the native structure of top antimalarial target PfATP4🧂led to the discovery of PfABP, an unknown essential binding partner, out now! @natcomms.nature.com 👉 rdcu.be/eLRlH 🦠🔬❄️
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
Endogenous structure of antimalarial target PfATP4 reveals an apicomplexan-specific P-type ATPase modulator
Nature Communications - Here, the authors present the 3.7 Å cryoEM structure of native sodium efflux pump PfATP4 from Plasmodium falciparum, revealing a bound protein that they term...
rdcu.be
October 20, 2025 at 12:55 PM
Learn how our hunt for the native structure of top antimalarial target PfATP4🧂led to the discovery of PfABP, an unknown essential binding partner, out now! @natcomms.nature.com 👉 rdcu.be/eLRlH 🦠🔬❄️
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
Reposted
1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from?
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Antiviral reverse transcriptases reveal the evolutionary origin of telomerase
Defense-associated reverse transcriptases (DRTs) employ diverse and distinctive mechanisms of cDNA synthesis to protect bacteria against viral infection. However, much of DRT family diversity remains ...
www.biorxiv.org
October 17, 2025 at 5:22 PM
1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from?
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Reposted
A beautiful piece of work from @ruixuan3.bsky.social and @supgokul.bsky.social presenting a light-based slicing method using a photosensitive hydrogel to image large biological samples layer by layer, preserving fine structure for full 3D reconstructions.
(1/x) Excited to report VIPS (Volumetric imaging of biological specimens via photochemical sectioning) @science.org! VIPS uses light-based sample sectioning to eliminate the inherent imaging depth limit of high-resolution optical microscopes @supgokul.bsky.social: www.science.org/doi/10.1126/...
Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning
Optical nanoscopy of intact biological specimens has been transformed by recent advancements in hydrogel-based tissue clearing and expansion, enabling the imaging of cellular and subcellular structure...
www.science.org
October 17, 2025 at 3:52 PM
A beautiful piece of work from @ruixuan3.bsky.social and @supgokul.bsky.social presenting a light-based slicing method using a photosensitive hydrogel to image large biological samples layer by layer, preserving fine structure for full 3D reconstructions.
As ever, beautiful #cryoEM work from the lab of Hong Zhou with field-defining insights using #cryoET:
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling
Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...
www.science.org
October 16, 2025 at 10:34 PM
As ever, beautiful #cryoEM work from the lab of Hong Zhou with field-defining insights using #cryoET:
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science
www.science.org/doi/10.1126/...
Reposted
If you're curious about how cells quickly eliminate misfolded proteins produced under proteotoxic stress or mitotic substrate during cell cycle progression, check out our latest article in Nature Communications:
doi.org/10.1038/s414...
doi.org/10.1038/s414...
Structural basis of K11/K48-branched ubiquitin chain recognition by the human 26S proteasome - Nature Communications
K11/K48 branched ubiquitin chains regulate protein degradation and cell cycle progression. Here, the authors report the structural basis of how such a branched ubiquitin chain is recognized by the hum...
doi.org
October 15, 2025 at 6:53 PM
If you're curious about how cells quickly eliminate misfolded proteins produced under proteotoxic stress or mitotic substrate during cell cycle progression, check out our latest article in Nature Communications:
doi.org/10.1038/s414...
doi.org/10.1038/s414...
Reposted
Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️
www.biorxiv.org/content/10.1...
#teamtomo
www.biorxiv.org/content/10.1...
#teamtomo
October 15, 2025 at 6:26 AM
Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️
www.biorxiv.org/content/10.1...
#teamtomo
www.biorxiv.org/content/10.1...
#teamtomo
Reposted
Lipid imaging on the cover of @nature.com! Great times for lipid cell biology indeed. And a fantastic recognition of all the hard work by the team, especially Juan M. Iglesias-Artola and Kristin Böhlig (who made the cover). Link to article: www.nature.com/articles/s41...
October 9, 2025 at 9:01 AM
Lipid imaging on the cover of @nature.com! Great times for lipid cell biology indeed. And a fantastic recognition of all the hard work by the team, especially Juan M. Iglesias-Artola and Kristin Böhlig (who made the cover). Link to article: www.nature.com/articles/s41...
Reposted
Ballistic Microscopy (BaM) www.biorxiv.org/content/10.1101/2025.10.07.681030v1 #cryoem
October 9, 2025 at 12:06 PM
Ballistic Microscopy (BaM) www.biorxiv.org/content/10.1101/2025.10.07.681030v1 #cryoem
Reposted
High-resolution #CryoET provides ‘compelling’ evidence for the true 3D architecture of glutamatergic synapses: a complex ‘synaptoplasm’ rather than the textbook postsynaptic density.
buff.ly/hOYKjHH
buff.ly/hOYKjHH
October 6, 2025 at 10:28 PM
High-resolution #CryoET provides ‘compelling’ evidence for the true 3D architecture of glutamatergic synapses: a complex ‘synaptoplasm’ rather than the textbook postsynaptic density.
buff.ly/hOYKjHH
buff.ly/hOYKjHH
Reposted
How does hunger spur us to consume? @columbiauniversity.bsky.social scientists have found a brain area that drives mice to consume sweets, fats, salt & food. This finding may inform novel treatments for both overeating and undereating:
zuckermaninstitute.columbia.edu/brain-dial-c...
zuckermaninstitute.columbia.edu/brain-dial-c...
September 10, 2025 at 3:56 PM
How does hunger spur us to consume? @columbiauniversity.bsky.social scientists have found a brain area that drives mice to consume sweets, fats, salt & food. This finding may inform novel treatments for both overeating and undereating:
zuckermaninstitute.columbia.edu/brain-dial-c...
zuckermaninstitute.columbia.edu/brain-dial-c...
Reposted
Progress in the development of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40896196/ #cryoem
September 3, 2025 at 10:19 AM
Progress in the development of an ultrafast pulsed ponderomotive phase plate for cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40896196/ #cryoem
Brooklyn at sunset
August 31, 2025 at 2:01 AM
Brooklyn at sunset
Our latest paper on the development of an #ultrafast #laser #PhasePlate for #cryoET @fitzpatricklab.bsky.social @zuckermanbrain.bsky.social
doi.org/10.1063/4.0000770
doi.org/10.1063/4.0000770
August 28, 2025 at 10:30 PM
Our latest paper on the development of an #ultrafast #laser #PhasePlate for #cryoET @fitzpatricklab.bsky.social @zuckermanbrain.bsky.social
doi.org/10.1063/4.0000770
doi.org/10.1063/4.0000770
Reposted
Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
August 27, 2025 at 4:14 PM
Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted
Contrast by electron microscopy in thick biological specimens pubmed.ncbi.nlm.nih.gov/40857101/ #cryoem
August 27, 2025 at 9:48 PM
Contrast by electron microscopy in thick biological specimens pubmed.ncbi.nlm.nih.gov/40857101/ #cryoem
Reposted
Optimal tilt-increment for cryo-ET www.biorxiv.org/content/10.1101/2025.08.20.671201v1 #cryoem
August 24, 2025 at 9:28 AM
Optimal tilt-increment for cryo-ET www.biorxiv.org/content/10.1101/2025.08.20.671201v1 #cryoem
Reposted
Fourier-based multiple-slice reconstruction in cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40834650/ #cryoem
August 22, 2025 at 5:05 AM
Fourier-based multiple-slice reconstruction in cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40834650/ #cryoem
Reposted
A massive genetic study in @Nature just identified why chronic pain hits some people harder than others.
They discovered the first neuronal “pain transporter” - and it could unlock entirely new ways to treat pain. 🧵
They discovered the first neuronal “pain transporter” - and it could unlock entirely new ways to treat pain. 🧵
August 22, 2025 at 7:03 AM
A massive genetic study in @Nature just identified why chronic pain hits some people harder than others.
They discovered the first neuronal “pain transporter” - and it could unlock entirely new ways to treat pain. 🧵
They discovered the first neuronal “pain transporter” - and it could unlock entirely new ways to treat pain. 🧵
Reposted
ICYMI: New online: Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug
Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug
Nature Structural & Molecular Biology, Published online: 18 August 2025; doi:10.1038/s41594-025-01632-3Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation elongation cycle and its perturbation by cabamiquine, a potent antimalarial drug candidate.
go.nature.com
August 19, 2025 at 11:17 AM
ICYMI: New online: Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug
New "Mindscapes" exhibit @zuckermanbrain.bsky.social featuring #cryoEM structures of #TMEM106B fibrils (doi.org/10.1016/j.cell.2022.02.026) and the ligand-bound human sweet receptor (doi.org/10.1016/j.cell.2025.04.021) from the Fitzpatrick (www.fitzpatrick-lab.org) and Zuker (www.zukerlab.com) labs
August 20, 2025 at 6:34 PM
New "Mindscapes" exhibit @zuckermanbrain.bsky.social featuring #cryoEM structures of #TMEM106B fibrils (doi.org/10.1016/j.cell.2022.02.026) and the ligand-bound human sweet receptor (doi.org/10.1016/j.cell.2025.04.021) from the Fitzpatrick (www.fitzpatrick-lab.org) and Zuker (www.zukerlab.com) labs
Reposted
Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH
A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo
A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo
Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug
Nature Structural & Molecular Biology - Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation...
rdcu.be
August 18, 2025 at 12:13 PM
Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH
A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo
A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo