Rob Campbell
@raacampbell.com
Reposted by Rob Campbell
Last night Adam Kampff, the glue, the light, the catalyst, the builder, the smile, left us. He and his work transformed the lives of many labs, scientists, students. He inspired and was generous to his last transformation, working tirelessly to set up a foundation to continue the work.Thank you!
December 9, 2025 at 3:37 PM
Last night Adam Kampff, the glue, the light, the catalyst, the builder, the smile, left us. He and his work transformed the lives of many labs, scientists, students. He inspired and was generous to his last transformation, working tirelessly to set up a foundation to continue the work.Thank you!
I doubt a new party is going to get 20% of the vote in any reasonable time frame. Certainly not by the next election, which it what we need to stop Farage.
September 19, 2025 at 12:58 PM
I doubt a new party is going to get 20% of the vote in any reasonable time frame. Certainly not by the next election, which it what we need to stop Farage.
Reposted by Rob Campbell
1/ Mapping synaptic connectivity between individual neurons is extremely laborious. We developed BRISC, a new method that makes it possible to map inputs onto 100s of neurons in the same animal in a matter of weeks! Led by Alex Becalick and @antblot.bsky.social. 🧵
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits
Sequencing of oligonucleotide barcodes holds promise as a high-throughput approach for reconstructing synaptic connectivity at scale. Rabies viruses can act as a vehicle for barcode transmission, than...
www.biorxiv.org
July 21, 2025 at 7:58 AM
1/ Mapping synaptic connectivity between individual neurons is extremely laborious. We developed BRISC, a new method that makes it possible to map inputs onto 100s of neurons in the same animal in a matter of weeks! Led by Alex Becalick and @antblot.bsky.social. 🧵
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Rob Campbell
🔬👨💻📰 #SReD is out!
Automated structural detection for #ImageJ & #FIJI, from nano to macro ✨🐘. No training data, no bias - texture analysis with GPU acceleration!⚡️
Brainchild of @afonsomendes92.bsky.social and adventure w @christlet.bsky.social lab + friends.
Check: www.nature.com/articles/s41...
Automated structural detection for #ImageJ & #FIJI, from nano to macro ✨🐘. No training data, no bias - texture analysis with GPU acceleration!⚡️
Brainchild of @afonsomendes92.bsky.social and adventure w @christlet.bsky.social lab + friends.
Check: www.nature.com/articles/s41...
July 2, 2025 at 7:09 AM
🔬👨💻📰 #SReD is out!
Automated structural detection for #ImageJ & #FIJI, from nano to macro ✨🐘. No training data, no bias - texture analysis with GPU acceleration!⚡️
Brainchild of @afonsomendes92.bsky.social and adventure w @christlet.bsky.social lab + friends.
Check: www.nature.com/articles/s41...
Automated structural detection for #ImageJ & #FIJI, from nano to macro ✨🐘. No training data, no bias - texture analysis with GPU acceleration!⚡️
Brainchild of @afonsomendes92.bsky.social and adventure w @christlet.bsky.social lab + friends.
Check: www.nature.com/articles/s41...
The first article begins with a short history of the approach
April 17, 2025 at 7:57 AM
The first article begins with a short history of the approach
Reposted by Rob Campbell
Curved light-sheet microscopy
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Curved light sheet microscopy for centimetre-scale cleared tissue imaging - Nature Photonics
Light sheet microscopy with curved light sheets enables tiling-free imaging of an entire intact cleared mouse brain with lateral and axial spatial resolutions of 1.0 μm and 2.5 μm, respectively, in le...
www.nature.com
April 12, 2025 at 10:21 PM
Curved light-sheet microscopy
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Rob Campbell
1/ Why do patients with late-stage cancer lose motivation & sink into apathy?
🔥 Our new Science paper shows chronic inflammation activates a cytokine-sensing brain circuit that lowers motivation. Huge team effort: Aelita Zhu, Sarah Starosta, Pignatelli & Janowitz labs! 🧵
🔗 doi.org/10.1126/scie...
🔥 Our new Science paper shows chronic inflammation activates a cytokine-sensing brain circuit that lowers motivation. Huge team effort: Aelita Zhu, Sarah Starosta, Pignatelli & Janowitz labs! 🧵
🔗 doi.org/10.1126/scie...
A neuroimmune circuit mediates cancer cachexia-associated apathy
Cachexia, a severe wasting syndrome associated with inflammatory conditions, often leads to multiorgan failure and death. Patients with cachexia experience extreme fatigue, apathy, and clinical depres...
doi.org
April 11, 2025 at 2:00 PM
1/ Why do patients with late-stage cancer lose motivation & sink into apathy?
🔥 Our new Science paper shows chronic inflammation activates a cytokine-sensing brain circuit that lowers motivation. Huge team effort: Aelita Zhu, Sarah Starosta, Pignatelli & Janowitz labs! 🧵
🔗 doi.org/10.1126/scie...
🔥 Our new Science paper shows chronic inflammation activates a cytokine-sensing brain circuit that lowers motivation. Huge team effort: Aelita Zhu, Sarah Starosta, Pignatelli & Janowitz labs! 🧵
🔗 doi.org/10.1126/scie...
Reposted by Rob Campbell
A huge day for mapping in life science!
—the mammalian brain, most extensive yet (10 papers @nature.com journals)
nature.com/articles/s41...
@alleninstitute.bsky.social
—a comprehensive map of the human cell->sub-cellular
nature.com/articles/s41...
—complete ape genomes
nature.com/articles/s41...
—the mammalian brain, most extensive yet (10 papers @nature.com journals)
nature.com/articles/s41...
@alleninstitute.bsky.social
—a comprehensive map of the human cell->sub-cellular
nature.com/articles/s41...
—complete ape genomes
nature.com/articles/s41...
April 9, 2025 at 9:34 PM
A huge day for mapping in life science!
—the mammalian brain, most extensive yet (10 papers @nature.com journals)
nature.com/articles/s41...
@alleninstitute.bsky.social
—a comprehensive map of the human cell->sub-cellular
nature.com/articles/s41...
—complete ape genomes
nature.com/articles/s41...
—the mammalian brain, most extensive yet (10 papers @nature.com journals)
nature.com/articles/s41...
@alleninstitute.bsky.social
—a comprehensive map of the human cell->sub-cellular
nature.com/articles/s41...
—complete ape genomes
nature.com/articles/s41...
Reposted by Rob Campbell
How does the brain work?
Scientists are closer to the answer with the largest wiring diagram and functional map of a mammalian brain to date. 🧵
🧠📈
Scientists are closer to the answer with the largest wiring diagram and functional map of a mammalian brain to date. 🧵
🧠📈
April 9, 2025 at 3:03 PM
How does the brain work?
Scientists are closer to the answer with the largest wiring diagram and functional map of a mammalian brain to date. 🧵
🧠📈
Scientists are closer to the answer with the largest wiring diagram and functional map of a mammalian brain to date. 🧵
🧠📈
Reposted by Rob Campbell
Today @nytscience.bsky.social on the microplastics (=5 bottle caps) in our brain, the recent @naturemedicine.bsky.social paper
gift link www.nytimes.com/2025/04/08/w...
gift link www.nytimes.com/2025/04/08/w...
April 8, 2025 at 1:59 PM
Today @nytscience.bsky.social on the microplastics (=5 bottle caps) in our brain, the recent @naturemedicine.bsky.social paper
gift link www.nytimes.com/2025/04/08/w...
gift link www.nytimes.com/2025/04/08/w...
This won't kill it, it will just hurt people with lower incomes. It needs to be carefully dismantled by enlightened governments. We are a bit short on those however.
April 5, 2025 at 9:54 AM
This won't kill it, it will just hurt people with lower incomes. It needs to be carefully dismantled by enlightened governments. We are a bit short on those however.
Reposted by Rob Campbell
Enhancing the Cre-loxP system: Two new genetic tools – roxCre and loxCre – can improve the performance of the Cre-loxP system for making genetic modifications in vivo.
elifesciences.org/reviewed-pre...
elifesciences.org/reviewed-pre...
April 3, 2025 at 3:21 AM
Enhancing the Cre-loxP system: Two new genetic tools – roxCre and loxCre – can improve the performance of the Cre-loxP system for making genetic modifications in vivo.
elifesciences.org/reviewed-pre...
elifesciences.org/reviewed-pre...
Reposted by Rob Campbell
Many of us use 2p scopes to image 3D volumes of brain. But then we analyze the data plane by plane, resulting in duplicated neurons, missed neurons, and low s/n. Let's go 3D!
Suite3D: Volumetric cell detection for two-photon microscopy
by @haydari.bsky.social & team.
www.biorxiv.org/content/10.1...
Suite3D: Volumetric cell detection for two-photon microscopy
by @haydari.bsky.social & team.
www.biorxiv.org/content/10.1...
April 1, 2025 at 11:10 AM
Many of us use 2p scopes to image 3D volumes of brain. But then we analyze the data plane by plane, resulting in duplicated neurons, missed neurons, and low s/n. Let's go 3D!
Suite3D: Volumetric cell detection for two-photon microscopy
by @haydari.bsky.social & team.
www.biorxiv.org/content/10.1...
Suite3D: Volumetric cell detection for two-photon microscopy
by @haydari.bsky.social & team.
www.biorxiv.org/content/10.1...
Reposted by Rob Campbell
I would encourage anyone asked to referee for a Royal Society journal, to very vocally follow Michael’s example. 🧪 ⚛️ 🔭
I have followed the #royalsociety #musk fellowship case over the last months, which culminated in this disappointment: https://www.theguardian.com/technology/2025/mar/25/royal-society-decides-not-to-take-disciplinary-action-against-elon-musk
As a consequence […]
[Original post on datasci.social]
As a consequence […]
[Original post on datasci.social]
March 30, 2025 at 4:17 PM
I would encourage anyone asked to referee for a Royal Society journal, to very vocally follow Michael’s example. 🧪 ⚛️ 🔭
I've got a kite with 800 m of line and I have used most of it on one occasion. So it must have been at 200 or 300 m.
March 30, 2025 at 5:24 PM
I've got a kite with 800 m of line and I have used most of it on one occasion. So it must have been at 200 or 300 m.
Vsauce puts all this well:
Illusions of Time
YouTube video by Vsauce
youtu.be
March 27, 2025 at 8:05 AM
Vsauce puts all this well:
Reposted by Rob Campbell
A practical guide for anyone using multiphoton microscopes: Assess your system performance with quick, affordable tests - ensuring reliable, standardised data across labs.
Out now in @natprot.bsky.social ⬇️
www.nature.com/articles/s41...
Out now in @natprot.bsky.social ⬇️
www.nature.com/articles/s41...
Standardized measurements for monitoring and comparing multiphoton microscope systems - Nature Protocols
A set of procedures is described here for standardizing the evaluation of multiphoton microscope performance, covering laser power, pulse width optimizations, field of view, resolution and photomultip...
www.nature.com
March 17, 2025 at 3:41 PM
A practical guide for anyone using multiphoton microscopes: Assess your system performance with quick, affordable tests - ensuring reliable, standardised data across labs.
Out now in @natprot.bsky.social ⬇️
www.nature.com/articles/s41...
Out now in @natprot.bsky.social ⬇️
www.nature.com/articles/s41...
Reposted by Rob Campbell
When I first joined here I remember there being a lot of posts about how the 'culture' of bluesky was to block liberally and starve the trolls.
I just hope people are still doing that!
I just hope people are still doing that!
March 17, 2025 at 1:19 PM
When I first joined here I remember there being a lot of posts about how the 'culture' of bluesky was to block liberally and starve the trolls.
I just hope people are still doing that!
I just hope people are still doing that!
Reposted by Rob Campbell
That was a fun project! Thanks to Rob Lees, Adam Packer, and all the co-authors for inviting me to participate.
rdcu.be/edQXi
rdcu.be/edQXi
Standardized measurements for monitoring and comparing multiphoton microscope systems
Nature Protocols - A set of procedures is described here for standardizing the evaluation of multiphoton microscope performance, covering laser power, pulse width optimizations, field of view,...
rdcu.be
March 17, 2025 at 12:08 PM
That was a fun project! Thanks to Rob Lees, Adam Packer, and all the co-authors for inviting me to participate.
rdcu.be/edQXi
rdcu.be/edQXi
Reposted by Rob Campbell
Everyone who is able should be doing this. And growing as much of their own food as they can, and saving seed
But individuals can only do so much, and millions don't have the means to stockpile or grow
The government should be making appropriate plans NOW
www.theguardian.com/commentisfre...
But individuals can only do so much, and millions don't have the means to stockpile or grow
The government should be making appropriate plans NOW
www.theguardian.com/commentisfre...
Britain’s food supply is precarious – and Trump’s chaos is spreading. I have a plan. Do you? | George Monbiot
I’ve started a stockpile, but that isn’t the answer. If crisis strikes, the government needs a strategy to protect us all, says Guardian columnist George Monbiot
www.theguardian.com
March 16, 2025 at 1:40 PM
Everyone who is able should be doing this. And growing as much of their own food as they can, and saving seed
But individuals can only do so much, and millions don't have the means to stockpile or grow
The government should be making appropriate plans NOW
www.theguardian.com/commentisfre...
But individuals can only do so much, and millions don't have the means to stockpile or grow
The government should be making appropriate plans NOW
www.theguardian.com/commentisfre...
Reposted by Rob Campbell
A #SciencePerspective by @zalaly.bsky.social and @erictopol.bsky.social identifies key issues that need to be prioritized in the study of #LongCovid.
Learn more on #LongCovidAwarenessDay: scim.ag/3XKbds3
Learn more on #LongCovidAwarenessDay: scim.ag/3XKbds3
March 15, 2025 at 2:07 PM
A #SciencePerspective by @zalaly.bsky.social and @erictopol.bsky.social identifies key issues that need to be prioritized in the study of #LongCovid.
Learn more on #LongCovidAwarenessDay: scim.ag/3XKbds3
Learn more on #LongCovidAwarenessDay: scim.ag/3XKbds3
Thanks. It was great to host you all.
March 15, 2025 at 8:12 AM
Thanks. It was great to host you all.
I returned two objectives to Nikon with damaged front elements. It took several months to get them back and cost was about 50% of new. A different time I returned an objective that seemingly spontaneously started to produce a horrible PSF. Took many months but was never charged.
March 14, 2025 at 7:03 AM
I returned two objectives to Nikon with damaged front elements. It took several months to get them back and cost was about 50% of new. A different time I returned an objective that seemingly spontaneously started to produce a horrible PSF. Took many months but was never charged.
Reposted by Rob Campbell
Are you using GCaMP8 or are planning to switch from GCaMP6? Then check out this new preprint from our lab! www.biorxiv.org/content/10.1...
- Calcium imaging
- GCaMP8 vs. GCaMP6
- Spike inference
- Imaging + ephys ground truth
With F. Helmchen, K. Svoboda, M. Rozsa & X. Fang.
- Calcium imaging
- GCaMP8 vs. GCaMP6
- Spike inference
- Imaging + ephys ground truth
With F. Helmchen, K. Svoboda, M. Rozsa & X. Fang.
March 11, 2025 at 11:30 AM
Are you using GCaMP8 or are planning to switch from GCaMP6? Then check out this new preprint from our lab! www.biorxiv.org/content/10.1...
- Calcium imaging
- GCaMP8 vs. GCaMP6
- Spike inference
- Imaging + ephys ground truth
With F. Helmchen, K. Svoboda, M. Rozsa & X. Fang.
- Calcium imaging
- GCaMP8 vs. GCaMP6
- Spike inference
- Imaging + ephys ground truth
With F. Helmchen, K. Svoboda, M. Rozsa & X. Fang.
Reposted by Rob Campbell
Please see our manuscript on a digital 3D bird brain atlas of a migratory and magnetoreceptive species (Eurasian blackcap) online now 🦅🧲🧠: biorxiv.org/content/10.1...
See also short thread👇
See also short thread👇
Mapping the magnetoreceptive brain: A 3D digital atlas of the migratory bird Eurasian blackcap (Sylvia atricapilla)
Birds undisputedly range amongst nature's foremost navigators. To successfully navigate between breeding and wintering quarters, they, in addition to other natural orientation cues, rely on their abil...
biorxiv.org
March 11, 2025 at 4:46 PM
Please see our manuscript on a digital 3D bird brain atlas of a migratory and magnetoreceptive species (Eurasian blackcap) online now 🦅🧲🧠: biorxiv.org/content/10.1...
See also short thread👇
See also short thread👇