Carballido-López's lab
@carballido-lab.bsky.social
Reposted by Carballido-López's lab
JB Editor's Choice: Anderson, Khursigara et al. review the role of peptidoglycomics in understanding the structure and function of peptidoglycan.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
December 2, 2025 at 8:54 PM
JB Editor's Choice: Anderson, Khursigara et al. review the role of peptidoglycomics in understanding the structure and function of peptidoglycan.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
Reposted by Carballido-López's lab
wohoo! FtsZ aficionadas/os take note 👇
#MicroSky
#MicroSky
Happy to share a new preprint from the lab: Marko became interested in whether the disordered linker of FtsZ might influence how the protein organizes into the Z-ring. And this led to some surprising findings!
Preprint: www.biorxiv.org/content/10.1...
Preprint: www.biorxiv.org/content/10.1...
Charge segregation in the C-terminal linker of FtsZ enables Z-ring scaling with prokaryotic cell width
Bacterial cytokinesis is orchestrated by the Z-ring, a cytoskeletal structure formed by treadmilling filaments of the tubulin-like GTPase FtsZ. During assembly, filament curvature must match the cell ...
www.biorxiv.org
November 26, 2025 at 10:09 PM
wohoo! FtsZ aficionadas/os take note 👇
#MicroSky
#MicroSky
Reposted by Carballido-López's lab
Really cool paper from @vincentdebakker.bsky.social @jonbakerlab.bsky.social now published @natcomms.nature.com #MicroSky www.nature.com/articles/s41...
Multi-omics profiling reveals atypical sugar utilization and a key membrane composition regulator in Streptococcus pneumoniae - Nature Communications
The pathogen Streptococcus pneumoniae can adapt to diverse microenvironments in the human body. Here, De Bakker et al. study these adaptation responses, showing unusual sugar utilization and identifyi...
www.nature.com
November 25, 2025 at 1:44 PM
Really cool paper from @vincentdebakker.bsky.social @jonbakerlab.bsky.social now published @natcomms.nature.com #MicroSky www.nature.com/articles/s41...
Reposted by Carballido-López's lab
Reposted by Carballido-López's lab
A two-component system signaling hub controls enterococcal membrane remodeling in response to daptomycin https://www.biorxiv.org/content/10.1101/2025.11.16.688641v1
November 17, 2025 at 6:17 PM
A two-component system signaling hub controls enterococcal membrane remodeling in response to daptomycin https://www.biorxiv.org/content/10.1101/2025.11.16.688641v1
Reposted by Carballido-López's lab
Interesting long read.
November 17, 2025 at 7:08 AM
Interesting long read.
Reposted by Carballido-López's lab
Spatial deconstruction of the plasma membrane https://www.biorxiv.org/content/10.1101/2025.11.14.688519v1
November 15, 2025 at 3:48 AM
Spatial deconstruction of the plasma membrane https://www.biorxiv.org/content/10.1101/2025.11.14.688519v1
Reposted by Carballido-López's lab
The cell envelope of diderm bacteria: a unified scaffold, not a stack of layers www.sciencedirect.com/science/arti... #jcampubs
November 10, 2025 at 4:39 PM
The cell envelope of diderm bacteria: a unified scaffold, not a stack of layers www.sciencedirect.com/science/arti... #jcampubs
Reposted by Carballido-López's lab
🔊 New story!
This GlmR (of hope) article has it all. Cell shape, cytokinesis, c-di-AMP, and catalysis - the famous 4 Cs 💎?
There is also antibiotic resistance, phosphorylation, acetylation, and a (cool) model to explain what shapes Bacillus cells.
Excellent group effort. Congrats team!
#Microsky
This GlmR (of hope) article has it all. Cell shape, cytokinesis, c-di-AMP, and catalysis - the famous 4 Cs 💎?
There is also antibiotic resistance, phosphorylation, acetylation, and a (cool) model to explain what shapes Bacillus cells.
Excellent group effort. Congrats team!
#Microsky
Staying in the loop to make ends meet: roles and regulation of GlmR in Bacillus subtilis https://www.biorxiv.org/content/10.1101/2025.11.05.686802v1
November 6, 2025 at 5:06 AM
🔊 New story!
This GlmR (of hope) article has it all. Cell shape, cytokinesis, c-di-AMP, and catalysis - the famous 4 Cs 💎?
There is also antibiotic resistance, phosphorylation, acetylation, and a (cool) model to explain what shapes Bacillus cells.
Excellent group effort. Congrats team!
#Microsky
This GlmR (of hope) article has it all. Cell shape, cytokinesis, c-di-AMP, and catalysis - the famous 4 Cs 💎?
There is also antibiotic resistance, phosphorylation, acetylation, and a (cool) model to explain what shapes Bacillus cells.
Excellent group effort. Congrats team!
#Microsky
Reposted by Carballido-López's lab
The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it!
www.fpbase.org/spectra/
www.fpbase.org/spectra/
FPbase Fluorescence Spectra Viewer
An interactive fluorescence spectra viewer to evaluate the spectral properties of fluorescent proteins, organic dyes, filters, and detectors.
www.fpbase.org
November 4, 2025 at 3:03 PM
The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it!
www.fpbase.org/spectra/
www.fpbase.org/spectra/
Reposted by Carballido-López's lab
Genome-wide screen identifies novel factors for surface protein cross-wall trafficking and cell envelope homeostasis in Staphylococcus aureus https://www.biorxiv.org/content/10.1101/2025.11.03.686427v1
November 5, 2025 at 2:16 AM
Genome-wide screen identifies novel factors for surface protein cross-wall trafficking and cell envelope homeostasis in Staphylococcus aureus https://www.biorxiv.org/content/10.1101/2025.11.03.686427v1
Reposted by Carballido-López's lab
Recruitment of the outer-membrane lipoprotein DolP to thedivision site via cardiolipin-mediated diffusion-stateswitching https://www.biorxiv.org/content/10.1101/2025.10.29.685289v1
October 30, 2025 at 2:17 AM
Recruitment of the outer-membrane lipoprotein DolP to thedivision site via cardiolipin-mediated diffusion-stateswitching https://www.biorxiv.org/content/10.1101/2025.10.29.685289v1
Reposted by Carballido-López's lab
Excited to share my latest postdoctoral work in the Shen Lab at Tufts! In this study, we follow up on an exciting finding by former grad student @shailab.bsky.social that C diff uses its Class A PBP (PBP1) to drive cell division (unlike previously studied bacteria)! journals.plos.org/plosgenetics...
Molecular dissection of Class A PBP function uncovers novel features of the non-canonical Clostridioides difficile divisome complex
Author summary Bacterial cell division is an ancient and essential process, but our molecular understanding of this process is primarily based on studies in a select few model systems. Recent work fou...
journals.plos.org
October 28, 2025 at 1:25 AM
Excited to share my latest postdoctoral work in the Shen Lab at Tufts! In this study, we follow up on an exciting finding by former grad student @shailab.bsky.social that C diff uses its Class A PBP (PBP1) to drive cell division (unlike previously studied bacteria)! journals.plos.org/plosgenetics...
Reposted by Carballido-López's lab
🦠🔬🤖🧑💻 #mAIcrobe is out! With @pinholab.bsky.social's lab, we launched an open-source framework for high-throughput bacterial image analysis. By rockstars A. Brito & B. Saraiva et al, making #DeepLearning for phenotyping accessible! Easy to use, plus model training
📜 www.biorxiv.org/content/10.1...
📜 www.biorxiv.org/content/10.1...
October 22, 2025 at 11:10 AM
🦠🔬🤖🧑💻 #mAIcrobe is out! With @pinholab.bsky.social's lab, we launched an open-source framework for high-throughput bacterial image analysis. By rockstars A. Brito & B. Saraiva et al, making #DeepLearning for phenotyping accessible! Easy to use, plus model training
📜 www.biorxiv.org/content/10.1...
📜 www.biorxiv.org/content/10.1...
Reposted by Carballido-López's lab
🚀🔬 Announcing #IMS2026 at @itqbnova.bsky.social! We're assembling a #LifeSciences #Microscopy symposium packed with ✨ speakers and 🧑🔬 workshops. All about imaging cells with photons, electrons and AI! If you love microscopy, you need to be here. Join us on March 19!
ims2026.itqbnovacommunity.org
ims2026.itqbnovacommunity.org
October 21, 2025 at 2:25 PM
🚀🔬 Announcing #IMS2026 at @itqbnova.bsky.social! We're assembling a #LifeSciences #Microscopy symposium packed with ✨ speakers and 🧑🔬 workshops. All about imaging cells with photons, electrons and AI! If you love microscopy, you need to be here. Join us on March 19!
ims2026.itqbnovacommunity.org
ims2026.itqbnovacommunity.org
Reposted by Carballido-López's lab
#MicrobiologyMonday: Staphylococcus aureus divides by building a septum and then splitting into 2 daughter cells. But how is septum formation coordinated with cell scission? Research in #mBio suggests cell wall mechanical stress could play a role: asm.social/2Eq
October 20, 2025 at 4:00 PM
#MicrobiologyMonday: Staphylococcus aureus divides by building a septum and then splitting into 2 daughter cells. But how is septum formation coordinated with cell scission? Research in #mBio suggests cell wall mechanical stress could play a role: asm.social/2Eq
Reposted by Carballido-López's lab
Job alert!
We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks?
See also on the forum:
forum.image.sc/t/research-e...
We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks?
See also on the forum:
forum.image.sc/t/research-e...
Research engineer in Bioimage Analysis for the researchers of the inIdEx FORMULA
Hi all We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks? The position is in the Instit...
forum.image.sc
October 7, 2025 at 12:39 PM
Job alert!
We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks?
See also on the forum:
forum.image.sc/t/research-e...
We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks?
See also on the forum:
forum.image.sc/t/research-e...
Reposted by Carballido-López's lab
We’re excited to announce the incredible speaker line-up for the Prokaryotic Cell Biology Conference! Join the leading researchers as they explore the latest advances in Nanoimaging, Cell Growth and Cellular Structures.
Join our speakers & register today! ➡️https://bit.ly/47rtkbw
#ProkaryoticCellBio
Join our speakers & register today! ➡️https://bit.ly/47rtkbw
#ProkaryoticCellBio
October 16, 2025 at 2:27 PM
We’re excited to announce the incredible speaker line-up for the Prokaryotic Cell Biology Conference! Join the leading researchers as they explore the latest advances in Nanoimaging, Cell Growth and Cellular Structures.
Join our speakers & register today! ➡️https://bit.ly/47rtkbw
#ProkaryoticCellBio
Join our speakers & register today! ➡️https://bit.ly/47rtkbw
#ProkaryoticCellBio
Reposted by Carballido-López's lab
Growth in confinement promotes Pseudomonas aeruginosa tolerance to antibiotics https://www.biorxiv.org/content/10.1101/2025.10.15.682596v1
October 15, 2025 at 11:17 PM
Growth in confinement promotes Pseudomonas aeruginosa tolerance to antibiotics https://www.biorxiv.org/content/10.1101/2025.10.15.682596v1
Reposted by Carballido-López's lab
News article in English: www.unil.ch/news/en/1760...
October 14, 2025 at 6:28 PM
News article in English: www.unil.ch/news/en/1760...
Reposted by Carballido-López's lab
Previous preprint is now published in RSC Chemical Biology.
If you are interested in plasma membrane labeling, see the paper! 👇
pubs.rsc.org/en/content/a...
If you are interested in plasma membrane labeling, see the paper! 👇
pubs.rsc.org/en/content/a...
September 8, 2025 at 8:02 AM
Previous preprint is now published in RSC Chemical Biology.
If you are interested in plasma membrane labeling, see the paper! 👇
pubs.rsc.org/en/content/a...
If you are interested in plasma membrane labeling, see the paper! 👇
pubs.rsc.org/en/content/a...
Reposted by Carballido-López's lab
Our MurJ/LysM paper has been published in Science Advances.
Kohga et al., Sci. Adv. (2025).
This study elucidates, through cryo-electron microscopy, the mechanism by which the small bacteriophage-derived protein LysM inhibits the lipid II flippase MurJ.
t.co/u5ZibBF0kA
Kohga et al., Sci. Adv. (2025).
This study elucidates, through cryo-electron microscopy, the mechanism by which the small bacteriophage-derived protein LysM inhibits the lipid II flippase MurJ.
t.co/u5ZibBF0kA
October 8, 2025 at 10:58 PM
Our MurJ/LysM paper has been published in Science Advances.
Kohga et al., Sci. Adv. (2025).
This study elucidates, through cryo-electron microscopy, the mechanism by which the small bacteriophage-derived protein LysM inhibits the lipid II flippase MurJ.
t.co/u5ZibBF0kA
Kohga et al., Sci. Adv. (2025).
This study elucidates, through cryo-electron microscopy, the mechanism by which the small bacteriophage-derived protein LysM inhibits the lipid II flippase MurJ.
t.co/u5ZibBF0kA
Reposted by Carballido-López's lab
Proud of our new study out in @cp-cellreports.bsky.social!
Using E. coli lacking all 8 endopeptidases, we provide direct evidence that peptidoglycan expansion during elongation requires ED-mediated insertion of one glycan strand at a time. This can be performed by MepS, MepM, MepH and PBP7 #microsky
Using E. coli lacking all 8 endopeptidases, we provide direct evidence that peptidoglycan expansion during elongation requires ED-mediated insertion of one glycan strand at a time. This can be performed by MepS, MepM, MepH and PBP7 #microsky
Role of endopeptidases in lateral cell wall expansion in Escherichia coli
Peptidoglycan, the major constituent of bacterial cell walls, is a giant macromolecule made of glycan strands cross-linked by short peptides, which pr…
www.sciencedirect.com
October 8, 2025 at 2:54 PM
Proud of our new study out in @cp-cellreports.bsky.social!
Using E. coli lacking all 8 endopeptidases, we provide direct evidence that peptidoglycan expansion during elongation requires ED-mediated insertion of one glycan strand at a time. This can be performed by MepS, MepM, MepH and PBP7 #microsky
Using E. coli lacking all 8 endopeptidases, we provide direct evidence that peptidoglycan expansion during elongation requires ED-mediated insertion of one glycan strand at a time. This can be performed by MepS, MepM, MepH and PBP7 #microsky
Reposted by Carballido-López's lab
Also see the accompanying paper from Carol Gross' lab that used a similar approach for Bacillus subtilis: www.cell.com/cell-systems...
Comprehensive genetic interaction analysis of the Bacillus subtilis envelope using double-CRISPRi
Koo et al. apply genome-scale double-CRISPRi to map cell envelope gene interactions
in Bacillus subtilis, revealing >1,000 genetic interactions and uncovering gene networks
in envelope biogenesis and ...
www.cell.com
October 4, 2025 at 8:48 AM
Also see the accompanying paper from Carol Gross' lab that used a similar approach for Bacillus subtilis: www.cell.com/cell-systems...
Reposted by Carballido-López's lab
The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.
A Protein Antibiotic Inhibits the BAM Complex to Kill Without Cell Entry
Many antibiotics are ineffective against Gram-negative pathogens such as Pseudomonas aeruginosa because they cannot penetrate the bacterial outer membrane. Here, we show that protein antibiotics calle...
www.biorxiv.org
September 20, 2025 at 9:29 AM
The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.