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Reposted by Robert Hänsel-Hertsch

David Vilchez @thevilchezlab.bsky.social · Sep 3

🚨 New from our lab: Here we explored how aging contributes to harmful protein aggregation in diseases like Huntington’s & ALS.
The culprit? An overlooked aging factor — EPS8 and RAC signaling. #Aging #Longevity #Proteostasis #ALSResearch
🧵
www.nature.com/articles/s43...

The aging factor EPS8 induces disease-related protein aggregation through RAC signaling hyperactivation - Nature Aging

Aging is a risk factor for neurodegenerative diseases associated with protein aggregation. Here the authors identify age-related hyperactivation of EPS8/RAC signaling in C. elegans as a driver of path...

www.nature.com

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Reposted by Robert Hänsel-Hertsch

David Vilchez @thevilchezlab.bsky.social · Sep 2

New work from the lab! C9orf72 ALS‐causing mutations lead to mislocalization and aggregation of nucleoporin Nup107 into stress granules - Bilican - FEBS Letters - Wiley Online Library febs.onlinelibrary.wiley.com/doi/10.1002/...

FEBS Press

Mutations in the C9orf72 gene represent the most common genetic cause of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Using patient-derived neurons and C. elegans models, w...

febs.onlinelibrary.wiley.com

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Aug 21

Incredible work, wow! Massive congratulations to you and your team!!!

Reposted by Robert Hänsel-Hertsch

Gianmaria Liccardi @gianmaria-liccardi.bsky.social · Aug 21

I am beyond excited and incredibly proud to share that our work is now out in @nature.com This discovery was made possible thanks to the first author Kostas kelepouras, my fantastic lab , and the unique research environment here in Cologne in collaboration with the Hospital bambino Gesu in Rome.

STING induces ZBP1-mediated necroptosis independently of TNFR1 and FADD - Nature

Nature - STING induces ZBP1-mediated necroptosis independently of TNFR1 and FADD

www.nature.com

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Aug 15

Thank you @genomeweb.bsky.social and Andrew P. Han for running a story on DynaTag. www.genomeweb.com/sequencing/b...
You can access the article with an academic/non-profit registration.

Buffer Tweak Opens Up CUT&Tag Chromatin Accessibility Assay to Measure Transcription Factor Binding

The simple reagent swap can enable single-cell and possibly even spatial profiling of transcription factor activity in tissues of many kinds.

www.genomeweb.com

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Aug 1

Congratulations Debora, fantastic news!

Trentini Lab @trentini-lab.bsky.social · Aug 1

🚨 Happy to share our first pre-print 🎉 on the causes of ribosome-associated degradation of CFTR and other transmembrane proteins.
We explore how protein folding, ER insertion, and elongation dynamics influence translation arrests in this new class of RQC targets.👇
www.biorxiv.org/content/10.1...

Principles of ribosome-associated protein quality control during the synthesis of CFTR

Prolonged translational arrests caused by defective mRNAs activate the ribosome-associated protein quality control (RQC) pathway, which marks harmful incomplete proteins for degradation. Multipass tra...

www.biorxiv.org

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Reposted by Robert Hänsel-Hertsch

Waggoner Lab @labwaggoner.bsky.social · Jul 29

DynaTag for efficient mapping of transcription factors in low-input samples and at single-cell resolution @natcomms.nature.com @epistrucstab.bsky.social
www.nature.com/articles/s41...

DynaTag for efficient mapping of transcription factors in low-input samples and at single-cell resolution - Nature Communications

Transcription factors shape cell identity, but mapping their genomic targets remains challenging. Here the authors present DynaTag, a modified CUT&Tag method for profiling TF occupancy in bulk and...

www.nature.com

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 29

Next one is coming up, will contact you soon!

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

Thanks Karolin, all the credit to Gloria Fuentes @gloglita.bsky.social.

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Reposted by Robert Hänsel-Hertsch

Stephen Matheson 🌵🚲 @sfmatheson.bsky.social · Jul 28

A great skeetorial on a new and much improved method for mapping transcription factor landscapes, with a beautiful image by our own Gloria Fuentes @gloglita.bsky.social from Life Science Editors @lifescienceeditors.bsky.social

Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

🧪Move over CUT&Tag, there’s a new #TranscriptionFactor mapping method in town.
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)

Image credit: @gloglita.bsky.social‬ @lifescienceeditors.bsky.social‬ captured DynaTag in action: a pA-Tn5 probe (multicoloured) binds an antibody (white), which binds p53 DNA-binding domain (green) on DNA (blue) within 2 nucleosomes

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Reposted by Robert Hänsel-Hertsch

CRC1678 @crc1678.bsky.social · Jul 28

🚀Big news from CRC 1678!
Robert Hänsel-Hertsch @epistrucstab.bsky.social and team unveil DynaTag—a single-cell method to map protein-DNA binding with stunning precision. Outshines ChIP-seq & CUT&RUN.
🧬Cancer research & gene regulation just got a major upgrade. #Genomics #DynaTag #PrecisionMedicine

Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

🧪Move over CUT&Tag, there’s a new #TranscriptionFactor mapping method in town.
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)

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Reposted by Robert Hänsel-Hertsch

CRC1678 @crc1678.bsky.social · Jul 28

🔬 DynaTag by CRC 1678 project leader Robert Hänsel-Hertsch @epistrucstab.bsky.social maps protein-DNA binding at single-cell level—boosting precision in cancer & gene research. #CRC1678 #DynaTag #UniCologne

Universität zu Köln @unicologne.bsky.social · Jul 28

🔬 New: Method for mapping protein-DNA interactions developed 💡
@epistrucstab.bsky.social #UniCologne has developed DynaTag, a method that detects the binding of proteins to DNA even in a single cell. This is crucial for gene regulation and the development of diseases like cancer. 🧬👇

uni.koeln/LXJQE

New method developed for mapping protein binding to DNA

A research team at the University of Cologne has developed a method called DynaTag, which can detect the binding of proteins to DNA, even in an individual cell. Interactions of proteins with DNA are c...

uni.koeln

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

Thank you Son for your invaluable help with the skeet! Your scientific illustrations look outstanding!

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Reposted by Robert Hänsel-Hertsch

Sonhita 🌞 @sonhita.bsky.social · Jul 28

🧪New method for anyone interested in protein-DNA interactions and TF mapping, from the lab of @epistrucstab.bsky.social at @unicologne.bsky.social!👇

Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

🧪Move over CUT&Tag, there’s a new #TranscriptionFactor mapping method in town.
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)

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Reposted by Robert Hänsel-Hertsch

Daniel Friedrich @friedrichlab.bsky.social · Jul 28

Highly recommended read for everyone interested in interactions of proteins and DNA: Impressive new method on single cell level introduced by @epistrucstab.bsky.social from @unicologne.bsky.social!

Universität zu Köln @unicologne.bsky.social · Jul 28

🔬 New: Method for mapping protein-DNA interactions developed 💡
@epistrucstab.bsky.social #UniCologne has developed DynaTag, a method that detects the binding of proteins to DNA even in a single cell. This is crucial for gene regulation and the development of diseases like cancer. 🧬👇

uni.koeln/LXJQE

New method developed for mapping protein binding to DNA

A research team at the University of Cologne has developed a method called DynaTag, which can detect the binding of proteins to DNA, even in an individual cell. Interactions of proteins with DNA are c...

uni.koeln

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Reposted by Robert Hänsel-Hertsch

Universität zu Köln @unicologne.bsky.social · Jul 28

Die neue Methode übertrifft bestehende Methoden wie #ChIP-seq und #CUT&RUN in Bezug auf Sensitivität und Auflösung der Ergebnisse. 📈
Die Studie wurde in ‪@natcomms.nature.com‬ veröffentlicht👇
www.nature.com/articles/s41...

DynaTag for efficient mapping of transcription factors in low-input samples and at single-cell resolution - Nature Communications

Transcription factors shape cell identity, but mapping their genomic targets remains challenging. Here the authors present DynaTag, a modified CUT&Tag method for profiling TF occupancy in bulk and...

www.nature.com

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Reposted by Robert Hänsel-Hertsch

Universität zu Köln @unicologne.bsky.social · Jul 28

🔬Neue Methode zur Kartierung von Proteinbindung an DNA entwickelt💡
@epistrucstab.bsky.social #UniKöln hat DynaTag entwickelt, eine Methode, die die Bindung von Proteinen an die DNA sogar in einer einzelnen Zelle nachweist 🧬👇https://uni.koeln/SCLU6

Neue Methode zur Kartierung von Proteinbindung an DNA entwickelt

Ein Forschungsteam der Universität zu Köln hat eine Methode mit dem Namen DynaTag entwickelt, die die Bindung von Proteinen an die DNA sogar in einer einzelnen Zelle nachweist. Interaktionen von Prote...

uni.koeln

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

Image credit: @gloglita.bsky.social @lifescienceeditors.bsky.social captured DynaTag in action: a pA-Tn5 probe (multicoloured) binds an antibody (white), which binds p53 DNA-binding domain (green) on DNA (blue) within 2 nucleosomes

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

Thanks to former PhD student Pascal Hunold and lab members Giulia Pizzolato & Olivia van Ray for their amazing work. Grateful to @dtg-cologne.bsky.social, labs of Peifer, George & Thomas, funders #CMMC @dfg.de #sfb1399 @crc1678.bsky.social #FOR5504 #Fritz_Thyssen_Foundation (7/7)

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

The University of Cologne press release can be found here:👇
www.uni-koeln.de/universitaet...
www.uni-koeln.de/en/universit... (6/7)

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

🗝️ #DynaTag can reveal nuanced TF occupancy behaviour and shed light on transcriptional regulation in health and disease. In small cell lung cancer #SCLC PDX models, it uncovered surprising gain-of-function p53, FOXA1 & MYC activity post-chemotherapy – not for ASCL1, NEUROD1, POU2F3 or YAP1 (5/7)

Adapted from Fig. 4 --> https://www.nature.com/articles/s41467-025-61797-9

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

How is DynaTag better than other technologies?
⚡Preserves weak/transient TF binding without signal loss
⚡Better signal-to-noise & resolution
⚡Works for all TFs (high and low DNA-binding affinity) & histone marks
⚡Low-input needed, bulk or single-cell, scalable to multi-cellular systems (4/7)

Fig.1f from https://www.nature.com/articles/s41467-025-61797-9

Fig.3f and g from https://www.nature.com/articles/s41467-025-61797-9

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

🤔 How to capture nuanced TF-DNA interactions?
💡 Enter #DynaTag: ‘Dynamic targets and Tagmentation’ captures transient TF-DNA interactions that standard CUT&Tag misses.
The trick? Sample prep under physiological salt stabilizes specific interactions without promoting untargeted tagmentation 🧂 (3/7)

Fig.1a from https://www.nature.com/articles/s41467-025-61797-9

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

❗TF occupancy mapping is still challenging. CUT&Tag was a breakthrough, but it has limits…
👉 Doesn’t work well for TFs that only transiently bind DNA
👉 These fleeting interactions are often lost during sample prep
That means we’re missing key regulatory events and it’s time for a new method (2/7)

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Robert Hänsel-Hertsch @epistrucstab.bsky.social · Jul 28

🧪Move over CUT&Tag, there’s a new #TranscriptionFactor mapping method in town.
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)

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