jamesdmanton.bsky.social
James Manton
@jamesdmanton.bsky.social
1.4K followers 560 following 370 posts
Applied physicist & microscopist/microscopologist
Posts Media Videos Starter Packs
Reposted by James Manton
retof.bsky.social
Reto Fiolka @retof.bsky.social · 2d
We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues.
www.biorxiv.org/content/10.1...
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Reposted by James Manton
jefferis.bsky.social
Greg Jefferis @jefferis.bsky.social · 3d
Exciting news for #drosophila #connectomics and #neuroscience enthusiasts: the Drosophila male central nervous system connectome is now live for exploration. Find out more at the landing page hosted by our Janelia FlyEM collaborators www.janelia.org/project-team....
Male CNS Connectome
A team of researchers has unveiled the complete connectome of a male fruit fly central nervous system —a seamless map of all the neurons in the brain and nerve cord of a single male fruit fly and the ...
www.janelia.org
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Reposted by James Manton
mrclmb.bsky.social
MRC Laboratory of Molecular Biology @mrclmb.bsky.social · 6d
Excellent opportunity for an enthusiastic #postdoc to join Kate McDole in @cellbiol-mrclmb.bsky.social, using interdisciplinary approaches to investigate tissue formation during early organogenesis in mouse embryos.
More info: www.nature.com/naturecareer...
Closes 20 OCT
#ScienceJobs #PostdocJobs
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Reposted by James Manton
buzzbaum.bsky.social
Buzz Baum @buzzbaum.bsky.social · 6d
LMB PhD studentship available in the Baum lab to use informatics to infer cell structure from genomic data in collaboration with the brilliant Tom Williams. If you write to me and I don’t reply - try again - the spam filter is hungry…
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Reposted by James Manton
immunoah.bsky.social
ImmuNoah's Ark (Noe Rodriguez) @immunoah.bsky.social · 7d
📣📣We're seeking a postdoc to join us at the @mrclmb.bsky.social to investigate neuroimmune interactomes in tumour & viral immunity.
If you find fascinating & want to learn about #neuroscience & #immunology, tracing neurons, molecular biology & #flowcytometry, apply!
🧠🫁👀🩸🔬
mrc.tal.net/vx/mobile-0/...
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Reposted by James Manton
biozentrum.unibas.ch
Biozentrum, University of Basel @biozentrum.unibas.ch · 9d
Assistant/Associate Professor in Structural Biology, Biophysics or Biological Imaging

www.biozentrum.unibas.ch/open-positio...

#biozentrum #University #Basel #Professor #Structural #Biology #Biophysics #Biological #Imaging
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Reposted by James Manton
superresolusian.bsky.social
Siân Culley @superresolusian.bsky.social · 9d
BioImage analysis friends - King's are recruiting for a full-time, permanent facility position! Come and work with fun microscopes and fun people (and me!) - please share! www.kcl.ac.uk/jobs/126345-...
Bioimage Analysis Specialist | King's College London
www.kcl.ac.uk
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Reposted by James Manton
adamkglaser.bsky.social
Adam Glaser @adamkglaser.bsky.social · 9d
Latest imaging data from our completely re-designed ExA-SPIM system.

Expansion-assisted imaging (3X expansion, 0.3 NA) enables brain-wide imaging with high-contrast and <400 nm effective resolution.
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jamesdmanton.bsky.social
James Manton @jamesdmanton.bsky.social · 9d
We were also concerned by that, so I thought we could use a cleared tissue lens and use Kramers–Kronig clearing to tune n to be just right.

Yeah, that doesn't seem to work... The gel shrinks quite a lot. I have a few more tricks to try before we give up, but you might also want to play with it.
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jamesdmanton.bsky.social
James Manton @jamesdmanton.bsky.social · 10d
If you want to go further, then @adamkglaser.bsky.social is the person to talk to!

bsky.app/profile/adam...
adamkglaser.bsky.social
Adam Glaser @adamkglaser.bsky.social · May 19
We are excited to share our development of a new 10X lens with NA=0.50 over a 7.2 mm FOV and a 35 mm working distance (water), fully corrected from 400-850 nm. This lens will power our upcoming ExA-SPIM "2" and is available for dissemination - please get in touch if interested!
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jamesdmanton.bsky.social
James Manton @jamesdmanton.bsky.social · 10d
@miguelcmestre.bsky.social is at the GEF25 with a poster on how we use them if you want a chat. He might also be able to give people a sneak-peek into the high-expansion-factor sectionable gel chemistries he's now working on to increase the effective working distance further still.
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jamesdmanton.bsky.social
James Manton @jamesdmanton.bsky.social · 10d
We're big fans of the Zeiss 40x/1.0 with 2.5 mm WD (www.micro-shop.zeiss.com/en/us/shop/o...) and the Nikon 60x/1.0 with 2.8 mm WD (www.microscope.healthcare.nikon.com/en_EU/produc...). Both work great on our inverted microscopes when using an FEP coverslip (www.biorxiv.org/content/10.1...).
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Reposted by James Manton
ewerslab.bsky.social
Ewerslab @ewerslab.bsky.social · 11d
Live stream of the talks at #GEF25! Experience the best of ExM first hand! Tomorrow 9 am it is me btw. Monday 9 am Ed Boyden himself!
alishaib.bsky.social
Ali Shaib @alishaib.bsky.social · 11d
#GEF25 LIVE BROADCAST

www.youtube.com/live/a_0X5Le...
Göttingen Expansion Forum 2025 Live Stream
YouTube video by Neuroscience and Beyond
www.youtube.com
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Reposted by James Manton
lapassmore.bsky.social
Lori Passmore @lapassmore.bsky.social · 11d
We have an open post-doc position in my group to study mRNA cleavage and polyadenylation using biochemical reconstitution and cryoEM.

Please get in touch if you are interested in joining this amazing team! 🔬🧬🤩
#RNA #cryoEM
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Reposted by James Manton
sventruckenbrodt.bsky.social
Sven Truckenbrodt @sventruckenbrodt.bsky.social · 16d
The Molecular Brain Mapping group at the MRC-LMB is hiring! 🌈🧠

I'm looking for people who are excited about revealing the many molecular layers of the "hidden connectome" with expansion microscopy, in a highly intersectional environment exploring ideas in meta-science.

mrc.tal.net/vx/mobile-0/...
Postdoctoral Scientist - Neurobiology - Dr Sven Truckenbrodt - LMB 2698 - Medical Research Council
Location: Cambridge. Vacancy: Postdoctoral Scientist - Neurobiology - Dr Sven Truckenbrodt - LMB 2698. Closing Date: 09/10/2025, 23:55
mrc.tal.net
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Reposted by James Manton
sventruckenbrodt.bsky.social
Sven Truckenbrodt @sventruckenbrodt.bsky.social · 16d
The Molecular Brain Mapping group at the MRC-LMB is hiring! 🌈🧠

I'm looking for people who are excited about developing multimodal analysis and visualization tools to capture the "hidden connectome," in a highly intersectional environment exploring ideas in meta-science.

mrc.tal.net/vx/mobile-0/...
Imaging Data Engineer - Neurobiology - Dr Sven Truckenbrodt - LMB 2697 - Medical Research Council
Location: Cambridge. Vacancy: Imaging Data Engineer - Neurobiology - Dr Sven Truckenbrodt - LMB 2697. Closing Date: 09/10/2025, 23:55
mrc.tal.net
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Reposted by James Manton
mrclmb.bsky.social
MRC Laboratory of Molecular Biology @mrclmb.bsky.social · 16d
Calling all prospective PhD students! Why not consider the LMB for your PhD?
Applications are now open!
www2.mrc-lmb.cam.ac.uk/students/int...
General deadline: 2nd DEC
US Gates Scholarship deadline: 15th OCT
Join the LMB's PhD programme! Applications now open for October 2026 Deadline 2nd December 2025
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Reposted by James Manton
dijiang319.bsky.social
Di Jiang @dijiang319.bsky.social · 16d
@science.org🧬🔬Functional maps of a #genomic locus reveal confinement of an enhancer by its target #gene | Science www.science.org/doi/10.1126/...
Functional maps of a genomic locus reveal confinement of an enhancer by its target gene
Genes are often activated by enhancers located at large genomic distances, and the importance of this positioning is poorly understood. By relocating promoter-reporter constructs into thousands of alt...
www.science.org
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Reposted by James Manton
djcohen.bsky.social
Daniel J. Cohen (Princeton) @djcohen.bsky.social · 21d
SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N
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Reposted by James Manton
christlet.bsky.social
Christophe 🔬 L @christlet.bsky.social · 21d
Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11
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Reposted by James Manton
geobellward.bsky.social
George Campbell @geobellward.bsky.social · 21d
After seeing
@miguelcmestre.bsky.social @lancasterlab.bsky.social @jamesdmanton.bsky.social
's paper this week on deep and accessible ExM imaging with cerebral organoids, I think it's a good time to repost this Starter Pack of Expansion Microscopy people!
go.bsky.app/Qxks9WD
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Reposted by James Manton
akaashkumar.bsky.social
Akaash Kumar @akaashkumar.bsky.social · 22d
🚨Publication alert🚨
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
Multispectral live-cell imaging with uncompromised spatiotemporal resolution - Nature Photonics
A tree-like arrangement of dichroic mirrors and multiple cameras coupled with an iterative spectral unmixing algorithm enables multispectral imaging of live cells in up to eight spectral channels with...
www.nature.com
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jamesdmanton.bsky.social
James Manton @jamesdmanton.bsky.social · 22d
The final version of our multispectral microscopy manuscript is now online at Nature Photonics: www.nature.com/articles/s41...

The main additions are some further comparisons to yet more unmixing algorithms, in which we find that our algorithm is still the most accurate.
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Reposted by James Manton
ibudin.bsky.social
Itay Budin @ibudin.bsky.social · 23d
Very excited to share new work out today in @natchembio.nature.com on a new approach - FACES - for selectively imaging of phospholipids and other biomolecules at spatial resolutions down to individual membrane leaflets (1/n) www.nature.com/articles/s41...
Leaflet-specific phospholipid imaging using genetically encoded proximity sensors - Nature Chemical Biology
An approach combining bioorthogonal chemistry with genetically encoded fluorogen-activating proteins enables subcellular imaging of phospholipids and glycans, as well as the visualization of lipid tra...
www.nature.com
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Reposted by James Manton
sventruckenbrodt.bsky.social
Sven Truckenbrodt @sventruckenbrodt.bsky.social · 23d
The cat is officially out of the bag!

The LMB is home to a unique community of outstanding connectomics scientists — I'm so excited about adding my own molecular and expansion microscopy expertise to the mix!

This is going to be the most exciting journey of my scientific career — more soon! 🌈🧠
mrclmb.bsky.social
MRC Laboratory of Molecular Biology @mrclmb.bsky.social · 23d
LMB welcomes @sventruckenbrodt.bsky.social as a new Group Leader in our Neurobiology Division!
Sven will develop molecular brain mapping, harnessing expansion microscopy & molecular biology methods to better understand the neural basis of behaviour.
www2.mrc-lmb.cam.ac.uk/the-lmb-welc... #LMBNews
Photograph of Sven Truckenbrodt wearing lab coat in laboratory setting.
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