Liam Horan-Portelance
@liamh-p.bsky.social
Postbac at NIA | synucleinopathy, single-cell/spatial transcriptomics | views are my own
Reposted by Liam Horan-Portelance
"We suggest this search is in vain. The problem lies not in the population but in the framework itself"
Shots fired!
@albertoespay.bsky.social & colleagues ask probing questions regarding αSyn-SAA-based staging for #Parkinsons progression
movementdisorders.onlinelibrary.wiley.com/doi/10.1002/...
Shots fired!
@albertoespay.bsky.social & colleagues ask probing questions regarding αSyn-SAA-based staging for #Parkinsons progression
movementdisorders.onlinelibrary.wiley.com/doi/10.1002/...
Refutation of the αSyn‐SAA‐Based Staging for Parkinson's Progression (Neuronal α‐Synuclein Disease‐Integrated Staging System [NSD‐ISS])
Click on the article title to read more.
movementdisorders.onlinelibrary.wiley.com
July 1, 2025 at 5:14 PM
"We suggest this search is in vain. The problem lies not in the population but in the framework itself"
Shots fired!
@albertoespay.bsky.social & colleagues ask probing questions regarding αSyn-SAA-based staging for #Parkinsons progression
movementdisorders.onlinelibrary.wiley.com/doi/10.1002/...
Shots fired!
@albertoespay.bsky.social & colleagues ask probing questions regarding αSyn-SAA-based staging for #Parkinsons progression
movementdisorders.onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Liam Horan-Portelance
Bipartisan letters from over 200 legislators urging "strong and sustained funding" for the NIH make me cautiously optimistic.
www.aamc.org/advocacy-pol...
www.aamc.org/advocacy-pol...
House, Senate Members Send Letters in Support of NIH Funding
Members of the House and Senate sent letters to appropriations leadership in support of NIH funding.
www.aamc.org
June 24, 2025 at 7:52 PM
Bipartisan letters from over 200 legislators urging "strong and sustained funding" for the NIH make me cautiously optimistic.
www.aamc.org/advocacy-pol...
www.aamc.org/advocacy-pol...
Reposted by Liam Horan-Portelance
"But I am blown away by how much we have learned about Alzheimer’s over the last couple of years."—Bill Gates
www.statnews.com/2025/06/17/b...
"This is the moment to spend more money on research, not less."
Agree 💯
erictopol.substack.com/p/predicting...
erictopol.substack.com/p/the-breakt...
www.statnews.com/2025/06/17/b...
"This is the moment to spend more money on research, not less."
Agree 💯
erictopol.substack.com/p/predicting...
erictopol.substack.com/p/the-breakt...
June 18, 2025 at 3:25 PM
"But I am blown away by how much we have learned about Alzheimer’s over the last couple of years."—Bill Gates
www.statnews.com/2025/06/17/b...
"This is the moment to spend more money on research, not less."
Agree 💯
erictopol.substack.com/p/predicting...
erictopol.substack.com/p/the-breakt...
www.statnews.com/2025/06/17/b...
"This is the moment to spend more money on research, not less."
Agree 💯
erictopol.substack.com/p/predicting...
erictopol.substack.com/p/the-breakt...
Reposted by Liam Horan-Portelance
What does the APOE 'lipid droplet-ome' look like???
Check out our new paper to find out!
www.sciencedirect.com/science/arti...
Thanks to first authors @cassifriday.bsky.social and @iostephens.bsky.social
+help from Scott Gordon @morganti.bsky.social and @cohenlaboratory.bsky.social labs
Check out our new paper to find out!
www.sciencedirect.com/science/arti...
Thanks to first authors @cassifriday.bsky.social and @iostephens.bsky.social
+help from Scott Gordon @morganti.bsky.social and @cohenlaboratory.bsky.social labs
APOE4 reshapes the lipid droplet proteome and modulates microglial inflammatory responses
Excess lipid droplet (LD) accumulation is implicated in various diseases, including Alzheimer's disease (AD), yet the mechanisms underlying this accum…
www.sciencedirect.com
June 3, 2025 at 1:09 AM
What does the APOE 'lipid droplet-ome' look like???
Check out our new paper to find out!
www.sciencedirect.com/science/arti...
Thanks to first authors @cassifriday.bsky.social and @iostephens.bsky.social
+help from Scott Gordon @morganti.bsky.social and @cohenlaboratory.bsky.social labs
Check out our new paper to find out!
www.sciencedirect.com/science/arti...
Thanks to first authors @cassifriday.bsky.social and @iostephens.bsky.social
+help from Scott Gordon @morganti.bsky.social and @cohenlaboratory.bsky.social labs
Reposted by Liam Horan-Portelance
A Perspective on a dynamic and multimodal framework to define microglial states from @rsankowski.bsky.social and Marco Prinz
www.nature.com/articles/s41...
www.nature.com/articles/s41...
A dynamic and multimodal framework to define microglial states - Nature Neuroscience
Sankowski and Prinz propose a classification framework for microglia states that considers the contextual plasticity of microglia. Their multimodal classification aligns a robust terminology with biol...
www.nature.com
May 20, 2025 at 4:37 PM
A Perspective on a dynamic and multimodal framework to define microglial states from @rsankowski.bsky.social and Marco Prinz
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Liam Horan-Portelance
Researchers performed a genome-wide CRISPR screen & identified candidate genes that modulated #Parkinsons-associated GCase activity; They find COMMD3 is essential for lysosomal GCase activity, & variants are associated with increased PD risk
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
April 11, 2025 at 6:21 PM
Researchers performed a genome-wide CRISPR screen & identified candidate genes that modulated #Parkinsons-associated GCase activity; They find COMMD3 is essential for lysosomal GCase activity, & variants are associated with increased PD risk
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Never want to hear UMAP structure is meaningless again😉
April 12, 2025 at 8:01 PM
Never want to hear UMAP structure is meaningless again😉
Reposted by Liam Horan-Portelance
Some troubling information
A red banner appeared on the NeMO (The Neuroscience Multi-omic) webpage.
"This repository is under review for potential modification in compliance with Administration directives."
What effing Administration directives relate to a neurosciece multi-omic repository?
1/3
A red banner appeared on the NeMO (The Neuroscience Multi-omic) webpage.
"This repository is under review for potential modification in compliance with Administration directives."
What effing Administration directives relate to a neurosciece multi-omic repository?
1/3
April 9, 2025 at 12:59 PM
Some troubling information
A red banner appeared on the NeMO (The Neuroscience Multi-omic) webpage.
"This repository is under review for potential modification in compliance with Administration directives."
What effing Administration directives relate to a neurosciece multi-omic repository?
1/3
A red banner appeared on the NeMO (The Neuroscience Multi-omic) webpage.
"This repository is under review for potential modification in compliance with Administration directives."
What effing Administration directives relate to a neurosciece multi-omic repository?
1/3
Reposted by Liam Horan-Portelance
CellPhoneDB v5 is officially published!
nature.com/articles/s41...
Very happy to have played a small role in it by developing some tools to help visualise the complex cell-cell interaction data!
#SingleCell
nature.com/articles/s41...
Very happy to have played a small role in it by developing some tools to help visualise the complex cell-cell interaction data!
#SingleCell
CellPhoneDB v5: inferring cell–cell communication from single-cell multiomics data - Nature Protocols
CellPhoneDB combines a curated database of receptor–ligand interactions with methods to infer cell–cell communication from single-cell expression data. This protocol update describes an expanded datab...
nature.com
March 31, 2025 at 11:56 PM
CellPhoneDB v5 is officially published!
nature.com/articles/s41...
Very happy to have played a small role in it by developing some tools to help visualise the complex cell-cell interaction data!
#SingleCell
nature.com/articles/s41...
Very happy to have played a small role in it by developing some tools to help visualise the complex cell-cell interaction data!
#SingleCell
Reposted by Liam Horan-Portelance
✨ Announcing a new glutamate indicator - iGluSnFR4! ✨
Launched as a pair, iGLuSnFR4s and iGluSnFR4f have high-sensitivity and fast activation/deactivation for recording synapses.
More on the indicators and what they are already revealing: www.biorxiv.org/content/10.1...
🧠📈
Launched as a pair, iGLuSnFR4s and iGluSnFR4f have high-sensitivity and fast activation/deactivation for recording synapses.
More on the indicators and what they are already revealing: www.biorxiv.org/content/10.1...
🧠📈
March 25, 2025 at 10:24 PM
✨ Announcing a new glutamate indicator - iGluSnFR4! ✨
Launched as a pair, iGLuSnFR4s and iGluSnFR4f have high-sensitivity and fast activation/deactivation for recording synapses.
More on the indicators and what they are already revealing: www.biorxiv.org/content/10.1...
🧠📈
Launched as a pair, iGLuSnFR4s and iGluSnFR4f have high-sensitivity and fast activation/deactivation for recording synapses.
More on the indicators and what they are already revealing: www.biorxiv.org/content/10.1...
🧠📈
Reposted by Liam Horan-Portelance
Reposted by Liam Horan-Portelance
Sympathetic and parasympathetic subtypes of body-first Lewy body disease observed in postmortem tissue from prediagnostic individuals 🧠🧪
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Sympathetic and parasympathetic subtypes of body-first Lewy body disease observed in postmortem tissue from prediagnostic individuals - Nature Neuroscience
This postmortem study identifies brain-first and two body-first subtypes in prediagnostic cases of Lewy body disease. It highlights sympathetic and parasympathetic pathways in body-first disease, adva...
www.nature.com
March 19, 2025 at 11:06 AM
Sympathetic and parasympathetic subtypes of body-first Lewy body disease observed in postmortem tissue from prediagnostic individuals 🧠🧪
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Liam Horan-Portelance
Make no mistake, it is the students and our future scientists that will be most hurt by these disastrous decisions.
Whole careers just won't happen. A generation of scientists will be lost. It's already started.
Whole careers just won't happen. A generation of scientists will be lost. It's already started.
“Our next generation of researchers are now poised on the edge of this cliff, not knowing if there’s going to be a bridge that’s going to get them to the other side, or if this is it”
Musk and Trump are blowing up science careers for every STEM student.
Musk and Trump are blowing up science careers for every STEM student.
Not only are we limiting our medical research and advancement of new treatments, but NIH cuts are affecting ability of universities to take on grad students, limiting our future workforce @altcdc.altgov.info @altnih4science.bsky.social #science #cuts #resist
apnews.com/article/trum...
apnews.com/article/trum...
March 8, 2025 at 2:04 PM
Make no mistake, it is the students and our future scientists that will be most hurt by these disastrous decisions.
Whole careers just won't happen. A generation of scientists will be lost. It's already started.
Whole careers just won't happen. A generation of scientists will be lost. It's already started.
Reposted by Liam Horan-Portelance
A single-cell atlas (>909k PBMCs) maps "strong sex-related differences" in gene-expression in blood from 121 healthy individuals, 48 patients with mild cognitive impairment (MCI), 46 with #Parkinsons, 27 with #Alzheimers, & 15 with both PD+MCI
www.nature.com/articles/s41...
www.nature.com/articles/s41...
February 26, 2025 at 10:41 PM
A single-cell atlas (>909k PBMCs) maps "strong sex-related differences" in gene-expression in blood from 121 healthy individuals, 48 patients with mild cognitive impairment (MCI), 46 with #Parkinsons, 27 with #Alzheimers, & 15 with both PD+MCI
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Liam Horan-Portelance
My weekend reading: New perspective "The genetics of neurodegenerative diseases is the genetics of age-related damage clearance failure"; #Parkinsons #Alzheimers
www.nature.com/articles/s41...
www.nature.com/articles/s41...
January 30, 2025 at 3:10 PM
My weekend reading: New perspective "The genetics of neurodegenerative diseases is the genetics of age-related damage clearance failure"; #Parkinsons #Alzheimers
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Liam Horan-Portelance
The data is the data, so don’t do an experiment unless you’re willing to accept evidence that goes against your hypothesis.
January 24, 2025 at 4:25 AM
The data is the data, so don’t do an experiment unless you’re willing to accept evidence that goes against your hypothesis.
Reposted by Liam Horan-Portelance
New paper presents a network-based systems genetics framework for drug repurposing for #Parkinsons; highlights Simvastatin & 11 other candidates
www.nature.com/articles/s41...
www.nature.com/articles/s41...
January 23, 2025 at 9:39 AM
New paper presents a network-based systems genetics framework for drug repurposing for #Parkinsons; highlights Simvastatin & 11 other candidates
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Liam Horan-Portelance
Neuronal micronuclei transfer to #microglia, altering microglial morphology and gene expression during the postnatal period in mice 🧠🧪
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Propagation of neuronal micronuclei regulates microglial characteristics - Nature Neuroscience
Neuronal micronuclei are transferred to microglia during the early postnatal period, which leads to altered microglial morphology and transcriptomic signatures, suggesting that these micronuclei may act as mediators that control microglial characteristics.
www.nature.com
January 21, 2025 at 3:07 PM
Neuronal micronuclei transfer to #microglia, altering microglial morphology and gene expression during the postnatal period in mice 🧠🧪
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Liam Horan-Portelance
Check out this paper and tool that we worked on with @sloanlab.bsky.social and Ye Zhang.
Think of it as 'the Barres datasets 2.0'
Lots of input and feedback from the amazing #Glia community.
Think of it as 'the Barres datasets 2.0'
Lots of input and feedback from the amazing #Glia community.
Hi #neuroscience community! This is one of my favorite posts ever. In close collaboration with @liddelowsa.bsky.social and Ye Zhang, we've built a new tool that we are eager to share! www.webseq.net
WEBseq
share freely
www.webseq.net
January 15, 2025 at 4:29 PM
Check out this paper and tool that we worked on with @sloanlab.bsky.social and Ye Zhang.
Think of it as 'the Barres datasets 2.0'
Lots of input and feedback from the amazing #Glia community.
Think of it as 'the Barres datasets 2.0'
Lots of input and feedback from the amazing #Glia community.
Reposted by Liam Horan-Portelance
A little video intro into Illumina Single Cell 3' RNA Prep protocols. It has begun!!
#singlecell
#transcriptomics
#PIPSeq
#illumina
#singlecell
#transcriptomics
#PIPSeq
#illumina
🧬Two updated technical videos demonstrating T2 and T20 workflows on newly released #Illumina Single-Cell 3' RNA Prep
T2: www.youtube.com/watch?v=4i5k...
T20: www.youtube.com/watch?featur...
📰 www.prnewswire.com/news-release...
T2: www.youtube.com/watch?v=4i5k...
T20: www.youtube.com/watch?featur...
📰 www.prnewswire.com/news-release...
January 13, 2025 at 7:54 AM
A little video intro into Illumina Single Cell 3' RNA Prep protocols. It has begun!!
#singlecell
#transcriptomics
#PIPSeq
#illumina
#singlecell
#transcriptomics
#PIPSeq
#illumina
Reposted by Liam Horan-Portelance
Incident dementia risk decreases at age 85-95 compared to 75-84. Dementia is not due to “brain biological aging,” it is caused by specific pathologies that peak in incidence in the eighth decade.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Lifetime risk and projected burden of dementia - Nature Medicine
A cohort study of over 15,000 US adults found that the lifetime risk of developing dementia from ages 55 to 95 is 42%, with the highest risk in APOE ε4 carriers, women and Black adults, and projected ...
www.nature.com
January 14, 2025 at 3:18 PM
Incident dementia risk decreases at age 85-95 compared to 75-84. Dementia is not due to “brain biological aging,” it is caused by specific pathologies that peak in incidence in the eighth decade.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Liam Horan-Portelance
Very excited to share our latest publication in @NatureGenet! Our manuscript shows how Mendelian Randomization (MR) isolates putatively causal links between cell-type specific gene expression & brain phenotypes.
www.nature.com/articles/s41...
Tweetorial below 👇
www.nature.com/articles/s41...
Tweetorial below 👇
January 14, 2025 at 8:22 AM
Very excited to share our latest publication in @NatureGenet! Our manuscript shows how Mendelian Randomization (MR) isolates putatively causal links between cell-type specific gene expression & brain phenotypes.
www.nature.com/articles/s41...
Tweetorial below 👇
www.nature.com/articles/s41...
Tweetorial below 👇
Reposted by Liam Horan-Portelance
Provocative start to 2025.
Journal Club 💭:
1) How do we reconcile that other microglia-deficient (eg, CSFR1-/-) models draw different conclusions? Could the remaining immune cells be compensating?
2) Why do these brains have synchronous neuron firing, but brain organoids lacking microglia do not?
Journal Club 💭:
1) How do we reconcile that other microglia-deficient (eg, CSFR1-/-) models draw different conclusions? Could the remaining immune cells be compensating?
2) Why do these brains have synchronous neuron firing, but brain organoids lacking microglia do not?
Typical development of synaptic and neuronal properties can proceed without microglia in the cortex and thalamus - Nature Neuroscience
Microglia are proposed to have a role in brain development through synaptic engulfment and paracrine signaling. O’Keeffe et al. show that certain neurodevelopmental processes attributed to microglia c...
www.nature.com
January 9, 2025 at 6:21 PM
Provocative start to 2025.
Journal Club 💭:
1) How do we reconcile that other microglia-deficient (eg, CSFR1-/-) models draw different conclusions? Could the remaining immune cells be compensating?
2) Why do these brains have synchronous neuron firing, but brain organoids lacking microglia do not?
Journal Club 💭:
1) How do we reconcile that other microglia-deficient (eg, CSFR1-/-) models draw different conclusions? Could the remaining immune cells be compensating?
2) Why do these brains have synchronous neuron firing, but brain organoids lacking microglia do not?
Reposted by Liam Horan-Portelance
[Astrocyte] 🤔🤔🤔
"previously proposed neuroprotective & neurotoxic astrocytes are transitional states rather than distinct subtypes" "Neuroprotective astrocytes are an intermediate state of the transition from a nonreactive to a neurotoxic state"
www.nature.com/articles/s43...
"previously proposed neuroprotective & neurotoxic astrocytes are transitional states rather than distinct subtypes" "Neuroprotective astrocytes are an intermediate state of the transition from a nonreactive to a neurotoxic state"
www.nature.com/articles/s43...
Modulating mTOR-dependent astrocyte substate transitions to alleviate neurodegeneration - Nature Aging
The authors show that neuroprotective and neurotoxic astrocytes represent transitional cellular substates present during neuroinflammation and that targeting mTOR in astrocytes reduces neurotoxicity, ...
www.nature.com
January 9, 2025 at 2:12 PM
[Astrocyte] 🤔🤔🤔
"previously proposed neuroprotective & neurotoxic astrocytes are transitional states rather than distinct subtypes" "Neuroprotective astrocytes are an intermediate state of the transition from a nonreactive to a neurotoxic state"
www.nature.com/articles/s43...
"previously proposed neuroprotective & neurotoxic astrocytes are transitional states rather than distinct subtypes" "Neuroprotective astrocytes are an intermediate state of the transition from a nonreactive to a neurotoxic state"
www.nature.com/articles/s43...