Embryonic stem cells for chicken and seven other avian species are derived.

This protocol describes a method for carrying out CUT&Tag-Direct in 96 well microplates for high-throughput processing of samples by a single operator with only standard lab...

Transcriptional regulation is tightly linked to chromatin organization, with H3K4me3 commonly marking both active and bivalent promoters. In embryonic stem cells (ESC), MLL2 is essential for H3K4me3 d...

Wang et al. show that the architectural protein LDB1 forms a complex with single-stranded DNA-binding proteins (SSBPs) in vitro and in vivo. SSBP3 stabilizes LDB1 self-interactions to support chromatin loop formation.

Durdu et al. crack the code of transcription-factor specificity by integrating chromatin context and motif syntax, validated by targeted assays. Focusing on NGN2 and MyoD1, they develop predictive models, distinguish functional binding from millions of potentials, and uncover generalizable rules explaining how transcription factors drive distinct cell fates.

Enhancers are non-coding cis-regulatory elements that control the expression of distally located genes in a tissue- and time-specific manner. Recent studies indicate that enhancers can differ in their...

The PRC1 complex regulates developmental gene expression in mammals by ubiquitinating histones (H2AK119ub) and nucleating repressive chromatin interactions. Human genetic data and functional experimen...

The REX element is associated with long-range enhancer–promoter interactions.

Cis-regulatory elements (CREs) have a major effect on phenotypes including disease. They are identified in a genome-wide manner by analyzing the binding of transcription factors (TFs), various co-fact...