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Reposted by Pablo Carravilla

Itay Budin @ibudin.bsky.social · Apr 24

Out today, we report how small chemical modifications in the metabolism of ergosterol are needed for yeast membranes to phase seperate into ordered yet fluid domains www.science.org/doi/10.1126/... (1/n)

Structural dissection of ergosterol metabolism reveals a pathway optimized for membrane phase separation

The metabolism of ergosterol shapes this lipid to allow the formation of fluid membrane domains in yeast cells.

www.science.org

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Reposted by Pablo Carravilla

Levental Lab @leventallab.bsky.social · May 19

Hey gang, stop what you’re doing, new organelle just dropped!!

Amazing the kind of stuff one finds inside cells by just looking at them (with magic like cryoET). What the heck are all these strange hemifused vesicles?

Ebrahim Lab @ebrahim-lab.bsky.social · May 17

Early for #MicroscopyMonday: #Hemifusomes- Newly discovered ESCRT-independent intermediates of intraluminal vesicle and multivesicular body formation! In collaboration with the Kachar Lab at the NIH and out today in @natcomms.nature.com : rdcu.be/emtQu

#cryoET #trafficking #organelle #hemifusion

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Reposted by Pablo Carravilla

Fabio Lolicato @fabiololicato.com · Mar 6

🚨 Postdoc Opportunity #2 🚨

We are looking for candidates with a strong background in molecular dynamics simulations of membrane protein interactions to unravel the role of lipids in CD95 oligomerization and signaling!

Please apply by March 28!

#LipidTime @bzh-hd.bsky.social

Health + Life Science Alliance Heidelberg Mannheim @hlsalliance.bsky.social · Mar 6

#PostDocCalls Project #85:
˚⋆🔬Lipid-Mediated Regulation of CD95 #Oligomerization and Signaling🧪🥽🧠⋆˚
Applications on the Alliance website:
www.health-life-sciences.de/opportunitie...
@dkfz.bsky.social
@fabiololicato.com @bzh-hd.bsky.social
@hlsalliance.bsky.social
#LipidTime #ProteinDesign

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Reposted by Pablo Carravilla

Dimova Lab @dimovalab.bsky.social · Feb 26

📢 SAVE THE DATE: Sept 29 - Oct 1, 2025
and come to Berlin to the

BIOMEMBRANE DAYS

Excellent line of speakers and slots for contributed talks.
Registration will open in Spring.

biomembrane-days-2025.mpikg.mpg.de

Organized by [email protected] Thomas Weikl and myself
@mpici.bsky.social

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Reposted by Pablo Carravilla

Erdinc Sezgin @sciezgin.bsky.social · Feb 19

New paper out 🎇
It has been 13 years since @owenlabuob.bsky.social and co. published a stellar protocol on how to measure membrane order with smart probes! Since then, there has been new dyes, imaging modes and biology! We wrote a protocol for Nature Protocols:
www.nature.com/articles/s41...

Measuring plasma membrane fluidity using confocal microscopy - Nature Protocols

Membrane fluidity is a crucial feature in understanding cellular physiology. This protocol describes a robust pipeline for measuring plasma membrane fluidity using confocal imaging and new environment...

www.nature.com

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Pablo Carravilla @pablo-carravilla.bsky.social · Feb 19

Thanks to everyone involved! Luca Andronico, @jan-schlegel.bsky.social, @sciezgin.bsky.social and the rest of the group. Also to the researchers from so many different fields (microscopy, dye chemistry, analysis, artificial models, cells...) that made this protocol possible.

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Pablo Carravilla @pablo-carravilla.bsky.social · Feb 19

We wrote a protocol to quantify plasma membrane fluidity using a confocal microscope 🔬
Hopefully those interested in measuring membrane biophysical properties in live cells find it useful.
We also provide an ImageJ/Fiji tool to make the analysis as easy as possible.
www.nature.com/articles/s41...

Measuring plasma membrane fluidity using confocal microscopy - Nature Protocols

Membrane fluidity is a crucial feature in understanding cellular physiology. This protocol describes a robust pipeline for measuring plasma membrane fluidity using confocal imaging and new environment...

www.nature.com

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Reposted by Pablo Carravilla

Gerry Hammond @gerryhammond.bsky.social · Feb 11

The most frequently asked question we get: do #lipidtime genetically encoded lipid biosensors compete out cells’ native effector proteins?

Victoria Holmes and Morgan Ricci led a paper in JCB that answers this question:

rupress.org/jcb/article/...

Read on for a brief thread.

Single-molecule lipid biosensors mitigate inhibition of endogenous effector proteins | Journal of Cell Biology | Rockefeller University Press

Holmes and colleagues show that AKT activation is blocked by commonly used PIP3 biosensors, but that this can be overcome by expressing biosensors at singl

rupress.org

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Pablo Carravilla @pablo-carravilla.bsky.social · Jan 26

We both went from black to gray, that's for sure! XD

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Pablo Carravilla @pablo-carravilla.bsky.social · Jan 26

Thanks Falk!!!!

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Pablo Carravilla @pablo-carravilla.bsky.social · Jan 26

Thanks so much André!

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Pablo Carravilla @pablo-carravilla.bsky.social · Jan 26

Thanks Ilya!

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Pablo Carravilla @pablo-carravilla.bsky.social · Jan 26

Thanks for your support throughout my research journey Erdinc, you have been a true inspiration for me!

Erdinc Sezgin @sciezgin.bsky.social · Jan 24

New PI born 🎁 In case you are one of the few who doesn't know @pablo-carravilla.bsky.social , he is starting his lab in Bilbao! Having Pablo in the lab as postdoc was a true privilege! Vividly remembering the first time we discussed this (Fig 1a) and today when he is ready to be a leader (Fig 1b).

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Reposted by Pablo Carravilla

Falk Schneider @faldalf.bsky.social · Dec 16

How does nano-scale plasma membrane structure underpin tissue development?
Let’s find out!

First opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP and University of Warwick.

🔬✨🐟📸

More info here:
www.findaphd.com/phds/project...

Temporal projection of a microscopy time-lapse image. Membranes of the cells in zebrafish brain at 20 hpf are labelled. Time is colour coded.

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Reposted by Pablo Carravilla

Falk Schneider @faldalf.bsky.social · Dec 13

‼️3 postdoc positions open‼️

We are building a quantitative imaging team at Warwick!
Join an interdisciplinary endeavor with Mishima, Balasubramanian, and Mayor labs on a @wellcometrust.bsky.social funded project to push probe and microscopy development!

warwick-careers.tal.net/vx/lang-en-G...

a logo that says let 's do this in blue and yellow

ALT: a logo that says let 's do this in blue and yellow

media.tenor.com

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Reposted by Pablo Carravilla

Julian Kompa @juliankompa.bsky.social · Nov 27

Hi Bluesky! 👋
Here is our latest collaborative work on long-term (up to 20 min) single-molecule tracking in living cells using exchangeable HaloTag Ligands 🔬
#ChemBio #microscopy

onlinelibrary.wiley.com/doi/10.1002/...

Long‐Term Single‐Molecule Tracking in Living Cells using Weak‐Affinity Protein Labeling

A self-labeling protein tag in combination with exchangeable fluorophore ligands bypasses photobleaching and enables long-term single-molecule imaging in living cells. This extends observation times ...

onlinelibrary.wiley.com

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Reposted by Pablo Carravilla

Joachim Goedhart @joachimgoedhart.bsky.social · Nov 25

The dark side of fluorescent protein tagging: the impact of protein tags on biomolecular condensation
www.biorxiv.org/content/10.1...

Figure 4. Position and type of fluorescent tag affect protein expression and P-body numbers in vivo.

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Pablo Carravilla @pablo-carravilla.bsky.social · Nov 25

Thanks for this! Could you add me plz?

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