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Paul Baker @pauljbaker.bsky.social · Feb 11

Shouya’s amazing efforts have provided functional validation of many reported variants of (previously) unknown significance in the #NLRP3 #inflammasome, not only uncovering interesting aspects of NLRP3 biology, but also hopefully leading to improved diagnosis and treatment of patients with CAPS!

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Paul Baker @pauljbaker.bsky.social · Feb 11

Congratulations to @shouyafeng.bsky.social @sethlucianmasters.bsky.social and the entire team on our lab’s latest publication out now in @natimmunol.bsky.social!
#autoinflammation #autoinflammatory

Seth Masters @sethlucianmasters.bsky.social · Feb 11

A large dataset we hope is helpful for patients who may have CAPS, an #autoinflammatory condition associated with genetic variants in NLRP3. Lots of "variants of uncertain significance" now have a specific readout from our in vitro assay which can help with clinical diagnosis.
rdcu.be/d9iRG

Mechanisms of NLRP3 activation and inhibition elucidated by functional analysis of disease-associated variants

Nature Immunology - Gain-of-function variants in the gene encoding NLRP3 lead to constitutive inflammasome activation and excessive IL-1β production. In this resource, authors perform...

rdcu.be

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Reposted by Paul Baker

Nature Reviews Rheumatology @natrevrheum.nature.com · Dec 20

#JanuaryIssue | Emerging concepts and treatments in autoinflammatory interferonopathies and monogenic systemic lupus erythematosus

Goldbach-Mansky and colleagues propose a pathogenesis-based classification of #autoinflammatory interferonopathies in this #Review

bit.ly/4gLCpOx

Emerging concepts and treatments in autoinflammatory interferonopathies and monogenic systemic lupus erythematosus - Nature Reviews Rheumatology

This Review provides a comprehensive update on dysregulated type I interferon production and signalling in autoinflammatory interferonopathies, monogenic systemic lupus erythematosus and conditio...

bit.ly

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Reposted by Paul Baker

Seth Masters @sethlucianmasters.bsky.social · Dec 14

Looks like a great inflammazoom coming up Dec 16 with @inflammasomelab.bsky.social and Si Ming Man! These talks are great for everyone interested in autoinflammation.

Register here to access the live session as well as on-demand talks from past sessions: events.abcam.com/AnYVL?rt=Qlp...

InflammaZoom

Join me online at the next InflammaZoom webinar!

events.abcam.com

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Reposted by Paul Baker

Inflammasome Lab @inflammasomelab.bsky.social · Dec 9

Have you ever wondered whether pyroptotic corpses have functions beyond the grave? We made the surprising discovery that pyroptotic corpses are flagged by #filopodia to alert #DendriticCells they need autopsy. A short Bluetorial (1/13)👇with movies!
Link: rdcu.be/d2mjm

Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells

Nature Immunology - Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate...

rdcu.be

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Paul Baker @pauljbaker.bsky.social · Dec 8

Thanks Matt! Our semi-regular debriefs all along the way were invaluable!

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Paul Baker @pauljbaker.bsky.social · Dec 7

Thanks Natalia!

Paul Baker @pauljbaker.bsky.social · Dec 7

Thanks to all of our amazing coauthors including @kerryhilligan.bsky.social, @danbarberphd.bsky.social for spending many, many hours tolerating me in the BSL3 lab, helping with experiments and data analysis, and providing valuable advice and moral support!

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Paul Baker @pauljbaker.bsky.social · Dec 7

More details in this 🧵 from my powerhouse PI @mayerbarber.bsky.social whose foresight and guidance got us to this fantastic manuscript!

Kat Mayer-Barber,PhD @mayerbarber.bsky.social · Dec 6

Best #Nikolaus 🎅! Our paper on how the 🫁 microenvironment can shape #innate immunity against #viruses is out @sciimmunology.bsky.social This was a herculean effort brilliantly led by @pauljbaker.bsky.social who singlehandedly established the model in the lab during the pandemic. 🧪 #Immunosky 1/9

Graphical summary of our paper. In mice, prior lower airway exposure to diverse inflammatory stimuli, including chronic bacterial infections such as M. tuberculosis, acute bacterial infections such as pulmonary S. aureus, viral infections such as Influenza A, type-II allergic responses such as the OVA-Alum model, activation of pulmonary TLR9 by CpG or pulmonary TLR1/2 by Pam3CSK4
leads to reduced viral burden upon subsequent infection with SARS-CoV-2 (SCV2). (2) This SCV2 restriction occurs prior to induction of SCV2-specific adaptive immune responses
and is mediated through innate immune responses, including the induction of IFN-I, TNFα and IL-1 and sustained changes to the TRM (Tissue resident macrophage) cellular
compartment and the pulmonary epithelium. (3) Innate cytokine and TLR signaling to both recruited immune cells and the pulmonary epithelium creates a microenvironment in the
lung that limits early replication of SCV2. IFN-I signaling to pulmonary ECs (epithelial cells) increases expression of interferon-stimulated genes, that likely cell-intrinsically limit viral
replication. TNF- or IL-1 suppress SCV2 independently of IFN-I signaling. TNF acts exclusively through radio-resistant cell types such as the lung epithelium, whereas IL-1 affords
control both direct and indirectly, through either stromal and hematopoietic cell types, to restrict overall early SCV2 burden.

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Paul Baker @pauljbaker.bsky.social · Dec 7

Excited to share this paper from my post-doc at @niaidnews.bsky.social out now in Science Immunology! We show that recent or ongoing 🫁 infections or other pulmonary environmental exposures at the time of infection with SARSCoV2 can vastly influence early replication of the virus in mice.

The inflammatory microenvironment of the lung at the time of infection governs innate control of SARS-CoV-2 replication

Recent or ongoing respiratory inflammation in the lung regulates antiviral immune responses to SARS-CoV-2.

www.science.org

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Reposted by Paul Baker

Seth Thomas Scanlon @immunoeditor.bsky.social · Dec 6

The December issue of #Science #Immunology is out now! This week: #MAITcell plasticity in #antibacterial immunity; how #inflammatory status of #lung before #COVID19 infection impacts #viral control; & #helminths utilize glutamate dehydrogenase to prevent #AAMs & #TH2 activation!

bit.ly/SI_DEC_24

This month’s cover features an adult female Hpb in the center, flanked by adult Hpb males with their characteristic caudal bursae on the left and right sides, crescent-shaped Hpb larvae from various stages surrounding the adults, and Hpb eggs in the four corners of the image.

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