Lightnews — Scholar-powered news

Rachael Ott

@rachaelott.bsky.social

2.4K followers 2.4K following 160 posts

Neurobiologist @UofSC 🔬 Super-resolution #microscopy, synaptic plasticity, cytoskeleton, #neurodegeneration 🦠

Posts Media Videos Starter Packs

Pinned

Rachael Ott @rachaelott.bsky.social · Jan 17

For #FluorescenceFriday, tau (blue) and long loops of neurofilament proteins are shown in a primary hippocampal neuron 🔬 #Neuroscience #Microscopy

Tau (blue) and neurofilament (fire) are shown in a primary hippocampal neuron.

3 25 180

Reposted by Rachael Ott

Journal of Cell Biology @jcb.org · Aug 11

Geng, @kjverhey1.bsky.social et al find that KIF1C forms condensates where the exposed #kinesin motor domains entangle nearby #microtubules, causing them to bend & break in a process dependent on motor processivity, cluster properties, cytoplasmic viscosity, & MT anchors rupress.org/jcb/article/...

2 13 50

Reposted by Rachael Ott

Manuel Thery @manuelthery.bsky.social · 1d

What would happen if cells would express more molecular motors ?
They would break all microtubules 😱
As shown by recent great work from @kjverhey1.bsky.social

rupress.org/jcb/article-...

14 65

Reposted by Rachael Ott

Reto Fiolka @retof.bsky.social · 1d

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues.
www.biorxiv.org/content/10.1...

5 43 110

Reposted by Rachael Ott

Shweta Nandakumar @bucklingmt.bsky.social · 2d

Playing around with @kwolbachia.bsky.social 's LUT manager plugin. Absolutely love the campfire LUT🔥 😍#microtubulemonday

2 4 21

Reposted by Rachael Ott

Ed Hutchinson @socialinfluenza.bsky.social · 2d

This is incredibly beautiful, and the fact that the resolution is sufficient to follow the path of DNA spooling inside the virion head and through the tail is amazing

The Daum Lab @daumlab.bsky.social · 2d

Out in Science Advances: Our #cryoEM structure of HFTV1, a virus infecting the halophile #archaea. *First full atomic structure (containing all structural proteins) of any tailed virus!* Congrats and thanks to all co-authors and our fantastic collaborators! www.science.org/doi/10.1126/...

Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail

This structure of an archaeal tailed virus (arTV) provides detailed insights into arTV assembly and infection mechanisms.

www.science.org

1 9 33

Rachael Ott @rachaelott.bsky.social · 3d

Thank you Anna!

Reposted by Rachael Ott

Rachael Ott @rachaelott.bsky.social · 5d

For #FluorescenceFriday, RhoA (blue) and the actin cytoskeleton (magenta) are shown in a set of primary microglia 🔬 #Neuroscience #Microscopy

4 16 81

Rachael Ott @rachaelott.bsky.social · 3d

Thank you Shweta! 😄

Reposted by Rachael Ott

Lori O'Brien @llobrienlab.bsky.social · 4d

Sharing this excellent review now out from postdoc Sarah McLarnon! Lots of useful info on 3D imaging, quantification of imaging data, and how this has been applied to the kidney. And since it's #FluorescenceFriday, sharing a figure with our own imaging data!
www.sciencedirect.com/science/arti...

Figure from our review paper showing various fluorescence microscopy images of cells and structures of the kidney

8 36

Reposted by Rachael Ott

Keri Martinowich @martinowk.bsky.social · 5d

For #FluorescenceFriday - simple image, but structure of hippocampus is beautiful with additional fanfare. 🟢 is Fos - labeling cells that get activated in response to disinhibition of inhibitory neuron subpopulation in the prefrontal cortex. Image credit to JHU Neuroscience student Aaron Salisbury.

smFISH for immediate early gene Fos in a section of the mouse hippocampus

5 46

Reposted by Rachael Ott

Danielle Beckman @daniellebeckman.bsky.social · 4d

👻 "Dark Forest Within" 🧠
What if the brain is a creepy forest? Our neurons are the 🌲, old (as old as we are!), and without mobility. Suddenly, comes the microglia, our brain's immune cells. Everything lights up when they move around.
🟢microglia🟣neurons
#FluorescenceFriday 🎃

Confocal microscopy photo showing neurons and microglia

4 19 98

Reposted by Rachael Ott

Kevin Terretaz @kwolbachia.bsky.social · 4d

I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager!
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...

6 34 92

Rachael Ott @rachaelott.bsky.social · 3d

Looks awesome! I can’t wait to try it out 🤩

Rachael Ott @rachaelott.bsky.social · 3d

Thank you! It’s a Santa Cruz antibody (26C4), diluted 1:200, and left overnight :)

Rachael Ott @rachaelott.bsky.social · 3d

Thanks for sharing Leah! 😁

Rachael Ott @rachaelott.bsky.social · 3d

Thank you! 😄

Rachael Ott @rachaelott.bsky.social · 5d

For #FluorescenceFriday, RhoA (blue) and the actin cytoskeleton (magenta) are shown in a set of primary microglia 🔬 #Neuroscience #Microscopy

4 16 81

Reposted by Rachael Ott

Journal of Cell Science @jcellsci.bsky.social · 5d

Mario Ledesma-Terrón, Diego Pérez-Dones, David Míguez and colleagues @cbm-csic-uam.bsky.social @ifimacuam.bsky.social present OSCAR, a framework to quantify 3D stacks with high cellular density.
journals.biologists.com/jcs/article-...

1 22 73

Reposted by Rachael Ott

Christophe 🔬 L @christlet.bsky.social · 5d

Nanotubes everywhere between neurons! A bit skeptical about these "dendrite-dendrite nanotubes" from a phalloidin staining in culture, but let's read on… www.science.org/doi/10.1126/...

1 8 38

Rachael Ott @rachaelott.bsky.social · 5d

Easily my new favorite image of yours!! Awesome work Maik!!

Reposted by Rachael Ott

Maik Bischoff @maikbischoff.bsky.social · 5d

My entry for today’s #FluorescenceFriday: a pupal #Drosophila testis with muscles expressing
🔵 lifeact &
🔴 RFP-nls

Honored & grateful to receive an honorable mention at @healthcare.nikon.com Nikon Small World 🌍🔬✨

🔗 www.nikonsmallworld.com/galleries/20...

#NikonSmallWorld #Microscopy #ScienceArt

11 40 210

Reposted by Rachael Ott

Kate Cavanaugh @katecavanaugh.bsky.social · 6d

See this? This = implanting mouse embryo. Usually this happens inside its mother and is invisible to us, but we can actually watch implantation ex vivo with the hope of understanding why implantation goes awry in embryos of older women. A 🧵...

11 68 220

Reposted by Rachael Ott

Ishier Raote @ishier.bsky.social · 8d

ER exit sites are dynamic ER subdomains that integrate signals, reshape membranes and steer secretion. Twelve groups in the field came together to map their diversity, regulation, unanswered questions and future directions.
www.nature.com/articles/s41...

Towards a unified framework for the function of endoplasmic reticulum exit sites - Nature Reviews Molecular Cell Biology

Endoplasmic reticulum exit sites (ERES) are specialized ER subdomains that regulate the export of secreted cargo. This Roadmap explores how ERES integrate biochemical and mechanical signals to coordinate trafficking and proposes a multidisciplinary strategy to investigate their function, including in disease.

www.nature.com

2 23 47

Reposted by Rachael Ott

c0nc0rdance @c0nc0rdance.bsky.social · 8d

You're watching 12 hrs of live cell imaging compressed into a GIF.

A multi-nucleated mouse pre-adipocyte tries to undergo mitosis, triggers a checkpoint because it has too much DNA, & undergoes apoptosis.

The last stage is called "blebbing", which is wonderfully descriptive.

(🔬: NanoLive Imaging)

3 18 86

Reposted by Rachael Ott

Ben Engel @cellarchlab.com · 12d

Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on!

#TeamTomo #PlantScience 🧪 🧶🧬 🌾
elifesciences.org/articles/105...
1/🧵

3 44 140