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Reposted by Structura Biotechnology

Jan-Hannes Schaefer @jhschaef.bsky.social · 3d

Helical Reconstruction of Amyloids in cryoSPARC?
We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social.
Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...

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Reposted by Structura Biotechnology

Krishna Reddy @krishnareddy.bsky.social · 27d

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Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q

Evolutionary analysis reveals the origin of sodium coupling in glutamate transporters - Nature Structural & Molecular Biology

Reddy et al. used ancestral protein reconstruction, cryo-electron microscopy and functional assays to elucidate how a secondary active transporter evolved to harness the energy of sodium gradients to ...

go.nature.com

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Reposted by Structura Biotechnology

Oli Clarke @olibclarke.bsky.social · 26d

This one is a bit of a departure from the usual and definitely a work in progress!

We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.

Read on for details... 1/x

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Structura Biotechnology @structurabio.bsky.social · 28d

Advanced #cryoEM workflows are easy in #CryoSPARC: use the Low-Level Results Interface to combine upstream extracted particles with downstream pose and CTF information, use a volume result from an intermediate iteration of a refinement to create a mask, and more! guide.cryosparc.com/application-...

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Oli Clarke @olibclarke.bsky.social · Aug 30

Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️

Upper - cryo-EM reconstruction of stomatin AQP1 complex. Lower - reconstruction of stomatin-UT-B complex.

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Reposted by Structura Biotechnology

NanoImaging Services @nanoimaging.bsky.social · Aug 27

🧪 Skip the trial and error for structure determination of soluble proteins. Join the #webinar 10/8 for a gene-to-structure case study of CYP3A4, a 57 kDa cytochrome P450 monooxygenase, solved to 3.1 Å by #cryoEM.

nimgs.zoom.us/webinar/regi...

Welcome! You are invited to join a webinar: Soluble Proteins in Focus: Preparing for Cryo-EM Success. After registering, you will receive a confirmation email about joining the webinar.

Are you using cryo-EM to look at small soluble proteins? Join Dr. Claudio Catalano, a Senior Scientist at NanoImaging Services, to get expert advice on how to set your project up for success. He wil...

nimgs.zoom.us

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Reposted by Structura Biotechnology

The Rosalind Franklin Institute @rosfrankinst.bsky.social · Aug 18

Exciting work from the Franklin, @ox.ac.uk, and @diamondlightsource.bsky.social has led to a new method for imaging small proteins (<50 kDa) using cryoEM. By using bifunctional, bispecific nanobody scaffolds, the team have successfully solved the smallest protein structure to date (14 kDa).

Quote from Dr. Mingda Ye, University of Oxford, highlighting the collaborative breakthrough in cryo-EM imaging of small proteins: “This idea was initiated when solving sub-50kDa protein structures by cryo-EM was almost impossible. To break this barrier, many world-class scientists in different fields have combined forces and it is a great honour to work with them to bring this game-changing tool into reality!” The background diagram outlines the scientific workflow used in the study.

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Reposted by Structura Biotechnology

David B. Sauer @davidbsauer.bsky.social · Aug 15

Di-Gembodies (dimerized nanobodies) for improved and duplexed CryoEM of small and challenging targets!

A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social

Out now in Nature Chemical Biology @natchembio.nature.com

www.nature.com/articles/s41...

Covalently constrained ‘Di-Gembodies’ enable parallel structure solutions by cryo-EM - Nature Chemical Biology

Disulfide-based dimerization of modified identical and heterologous nanobody scaffolds enables higher-order assembly for high-resolution cryo-electron microscopy structure determination that is widely...

www.nature.com

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Reposted by Structura Biotechnology

Basil Greber @bjgreber.bsky.social · Aug 15

Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone!
www.nature.com/articles/s41...

High-throughput investigation of cyclin docking interactions reveals the complexity of motif binding determinants - Nature Communications

Many protein–protein interactions depend on Short Linear Motifs (SLiMs). In this study, the authors use large-scale binding assays, deep mutational scanning, and structural analysis to map SLiMs recog...

www.nature.com

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Structura Biotechnology @structurabio.bsky.social · Aug 5

Flexibility can be frustrating when processing a #cryoEM dataset. If your target has a wobbly domain, check out our new case study on FaNaC1 for continuous heterogeneity tips in #CryoSPARC!

guide.cryosparc.com/processing-d...

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Reposted by Structura Biotechnology

EMBO @embo.org · Jul 29

Get hands-on training on #cryofreezing, #DataAcquisition and more at EMBO Practical Course "Cryo electron microscopy and 3D image processing" in New Delhi, IN, 21 Feb–3 Mar 2026

Registration by 15 Sep
Abstract submission by 30 Sep

meetings.embo.org/event/26-cem...
#cryoEM #EMBOCryo3D #EMBOevents 🧪

Cryo electron microscopy and 3D image processing (CEM3DIP)

This EMBO Practical Course is for participants who are interested in analysis of macromolecular structures in vitro and in situ using cryo-electron microscopy single particle reconstruction and t…

meetings.embo.org

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Reposted by Structura Biotechnology

Miro Astore @miroastore.bsky.social · Jul 23

That's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv!
biorxiv.org/content/10.110

biorxiv.org

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Structura Biotechnology @structurabio.bsky.social · Jul 23

When particles have orientation bias, the resulting maps are often streaked along the missing directions. In this case study, we cover why orientation bias produces anisotropic #cryoEM maps and how you may be able to recover from it!

guide.cryosparc.com/processing-d...

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Structura Biotechnology @structurabio.bsky.social · Jul 14

guide.cryosparc.com/processing-d...

Case Study: Pseudosymmetry in TRPV5 and Calmodulin (EMPIAR-10256) | CryoSPARC Guide

Processing EMPIAR-10256 with a focus on handling pseudosymmetry and 3D Classification parameter choices.

guide.cryosparc.com

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Structura Biotechnology @structurabio.bsky.social · Jul 14

Link here! guide.cryosparc.com/processing-d...

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Structura Biotechnology @structurabio.bsky.social · Jul 14

Working with symmetric or pseudosymmetric particles in #cryoEM?

This TRPV5 and CaM case study includes practical considerations for using symmetry relaxation and 3D classification when analyzing a pseudosymmetric particle in #CryoSPARC!

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Oli Clarke @olibclarke.bsky.social · Jul 7

When I arrived to NYC as a postdoc, NYSBDG was my first local meeting, and I was blown way by the world-class quality of the science.

It's a great opportunity to meet the local #structbio & #cryoem community, and it's coming up in two weeks, so register now at the eventbrite link below!

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Structura Biotechnology @structurabio.bsky.social · Jun 24

Looking for a deep dive into 2D classification in #cryoEM?

These recent guide pages cover practical aspects & parameter choices when running these jobs in #CryoSPARC, along with the theory behind expectation maximization: guide.cryosparc.com/processing-d... and guide.cryosparc.com/expectation-...

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Reposted by Structura Biotechnology

Oli Clarke @olibclarke.bsky.social · Jun 12

1/x New story - the structure of the Vault cap, at 2.3Å! Matching a beautiful preprint from @sjorsscheres.bsky.social, we show that the cap of the vault particle has C13 symmetry, in contrast to the 39-fold symmetry of the body. Study lead by talented postdoc @huanli00.bsky.social from my group! ⬇️

At left, a reconstruction of the vault cap with C39 symmetry imposed; at right, the reconstruction after identifying the correct symmetry, with the three non-equivalent protomers highlighted.

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Reposted by Structura Biotechnology

U-M Life Sciences Institute @umlifesciences.bsky.social · Jun 9

Thank you to all the presenters and scientists from around the country who attended the LSI's 2025 #CryoEM Workshop and made the event such a successful hands-on learning experience!
Thanks also to our event sponsors, SubAngstrom, @globusorg.bsky.social, @mitegen.com and @structurabio.bsky.social

Group photo of the 2025 Cryo-EM Workshop participants, alongside a list of logos of the workshops sponsors: Globus, MiTeGen, Structura, SubAngstrom, and the Life Sciences Institute

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Reposted by Structura Biotechnology

Jason McLellan @mclellanlab.bsky.social · May 20

Check out our latest study, led by grad student Ryan McCool @rmccool.bsky.social‬, in collaboration with Vaccine Company. We were able to stabilize EBV gB in the prefusion conformation, determine its cryo-EM structure to 3.1 A, and assess its immunogenicity in mice. www.biorxiv.org/cgi/content/...

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Reposted by Structura Biotechnology

John Rubinstein @johnrubinstein.bsky.social · May 16

EtfD links beta oxidation to OxPhos in mycobacteria - recent work suggests that targeting EtfD could shorten #tubeculosis treatment.
But how does EtfD work? And how can you assay its activity?
In his final PhD manuscript @courbongautier.bsky.social provides answers!
www.biorxiv.org/content/10.1...

A) Diagram showing how EtfD transfers electrons from beta oxidation to the menaquinol pool in the membrane, linking beta oxidation to oxidative phosphorylation in mycobacteria.
B) A growth curve showing the Mycobacterium smegmatis lacking EtfD can grow when glucose is provided as the carbon source, but has a slight growth delay. Complementing the ∆etfD strain with M. tuberculosis on a plasmid rescues the defect.
C) A growth curve showing that ∆etfD M. smegmatis cannot grow on medium where the fatty acid oleic acid is the only carbon source. A plasmid with with M. tuberculosis EtfD rescues the defect.

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Reposted by Structura Biotechnology

Yi-Wei Chang @yiweichang.bsky.social · May 15

Excited to share our new @science.org paper! Led by postdocs Ruchao Peng and Xin Xu, we used cryo-EM/ET to reveal the influenza ribonucleoprotein complex structure and its strand-sliding mechanism for RNA synthesis, paving the way for new antivirals.
www.science.org/doi/10.1126/...

Molecular basis of influenza ribonucleoprotein complex assembly and processive RNA synthesis

Influenza viruses replicate and transcribe their genome in the context of a conserved ribonucleoprotein (RNP) complex. By integrating cryo–electron microscopy single-particle analysis and cryo–electro...

www.science.org

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Structura Biotechnology @structurabio.bsky.social · Apr 29

Grappling with poorly-resolved ligand binding sites in #cryoEM maps?
Check out our new case study on a peptide ligand-bound GPCR showing how practical tips and features in #CryoSPARC v4.7 improve locally refined map quality and reveal clearer ligand density!
guide.cryosparc.com/processing-d...

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Structura Biotechnology @structurabio.bsky.social · Apr 16

Are you new to #CryoEM, or looking for a review of the basics?

You might enjoy our TRPV1 case study! It covers preprocessing, particle curation, refinement, and more! You can also test your skills with the advanced exercises at the end of the study.
guide.cryosparc.com/processing-d...

#CryoSPARC

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