Takehiro A. Ozawa
@tkozawa.bsky.social
320 followers 740 following 32 posts
PhD candidate at CINVESTAV Unidad Irapuato 🇲🇽. Interested in microalgae (e.g. Botryococcus braunii), algal biotechnology and plant energy management (i.e. #PlantTOR and #SnRK1 signaling pathways 🌱⚡️).
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Reposted by Takehiro A. Ozawa
leocastanedo.bsky.social
🛑 Some 🌱 have the remarkable ability to tolerate and accumulate ☣️ metals.

Below 👇🏼 we present evidence that this trait depends on co-option of the circadian 🕰️ machinery❗

We found that co-option of 🕰️ proteins governs both enhanced and time-dependent expression of metal-accumulation genes❗
mplantpcom.bsky.social
Cis-regulatory elements co-opting core circadian clock regulator CCA1 underlie enhanced expression of HMA4 for metal hyperaccumulation in Arabidopsis halleri #research #PlantCommunications cell.com/plant-commun...
Reposted by Takehiro A. Ozawa
mingyuanzhu.bsky.social
🌱 Join us for our upcoming workshop Quantifying Plant Morphogenesis: 4D Insights with MorphoGraphX!
Learn how to quantitatively analyze plant confocal imaging data using MorphoGraphX.
Reposted by Takehiro A. Ozawa
rheelab.bsky.social
Only 10 days left to apply to the MSU Plant Resilience Institute for a faculty position.We're looking for 1 junior and 1 senior faculty. Applications submitted after 10/15 cannot be considered. Join us! It's a very collaborative, fun, innovative group of plant biologists. Apply at: lnkd.in/eRyDv_Pi
Open rank faculty positions available at MSU Plant Resilience Institute. Apply online at: https://tinyurl.com/yy2kvt3n
Reposted by Takehiro A. Ozawa
chenxinli2.bsky.social
Our new short article is online at Plant Biotech J (onlinelibrary.wiley.com/doi/10.1111/...)!

"Single Cell Multi-Omics Reveals Rare Biosynthetic Cell Types in the Medicinal Tree Camptotheca acuminata"

Product of an NSF-EAGER award that I was co-PI from 2023-2025 (www.nsf.gov/awardsearch/...).
Figure of the paper.
Reposted by Takehiro A. Ozawa
johninnescentre.bsky.social
VACANCY - Independent research fellowships leading to tenured positions

We’re inviting applications from outstanding researchers who either hold, or wish to apply for, Independent Research Fellowships.

APPLICATION DEADLINE: 10 November 2025

Click here to apply: jic.link/Fellows
Reposted by Takehiro A. Ozawa
hueihsuantsai.bsky.social
Very excited to see our @nikogeldner.bsky.social lab x Feng Zhou lab work featured on the cover of Science!
(1/5) We reveal how root architecture and nutrient leakage shape spatial patterns of microbial colonization, moving beyond traditional models of uniform exudation.
science.org
Using precise spatial and temporal analysis, researchers in Science provide insight into how bacteria around the root interact both with the plant and with each other.

Learn more in this week's issue: https://scim.ag/3WgNajk
A confocal microscopy image shows root-colonizing bacteria clustering around an emerging lateral root, where localized glutamine leakage induces spatially confined reporter activity.
tkozawa.bsky.social
Great preprint by Upadhyaya et al. (2025) on using a #multiomics approach to study #PlantTOR signaling in the unicellular green alga #Chromochloris zofingiensis, particularly showing the upregulation of #AminoAcid biosynthesis pathways during TOR inhibition with AZD8055.
#Algae #Phosphoproteomics 🔗⬇️
Fig. 8. Summary of the mechanism of TOR inhibition-dependent accumulation of amino acids in Chromochloris zofingiensis.

TOR inhibition by AZD8055 induces the expression of transcription factors G2- like, HSF, and bHLH, which bind to amino acid biosynthesis and nitrogen metabolism genes to potentially regulate their transcription.
This process, which is independent of glucose-TOR signaling, leads to an accumulation of amino acids during TOR inhibition. Glucose addition via hexokinase1 (HXK1) also upregulates amino acid biosynthesis. Fig. 1. TOR inhibition prevents glucose-driven growth and suppresses photosynthesis in the green alga Chromochloris zofingiensis.

(A) Representative C. zofingiensis (WT) cultures over the 96 h experiment with the following treatments: control, TOR inhibition by 1 µM AZD8055, 100 mM glucose addition, and 100mM glucose + 1 µM AZD treatment.

(B) Cell density and (C) maximum PSII photosynthetic efficiency (Fv/Fm). For (B) and (C), datapoints represent biological replicates with lines representing means (n = 3).

(D) PCA of WT 1 h and 24 h transcriptome, proteome, and metabolome with treatments: control, 1 µM AZD, 15 mM glucose, 15 mM glucose + 1 µM AZD, and 15 mM sorbitol as an osmotic control. 
For the transcriptome, normalized log2-transformed counts of 500 genes with highest variance were considered.
For the proteome and the metabolome, the raw intensity values were log2-transformed, and proteins or metabolites with highest variance were used to calculate and plot the PCA. Modified Fig. 2. Phosphoproteomics of TOR-inhibited Chromochloris zofingiensis identifies novel TOR players. 

(B) The log2 fold changes of TOR-dependent phosphosites between TOR inhibition by 1 µM AZD, 15 mM glucose addition, 15 mM glucose + 1 µM AZD, and 15 mM sorbitol-treated C. zofingiensis (WT) cultures at 1 h and 24 h from cluster 4 were labelled according to their protein symbols and phosphorylated Ser/Thr residue on the left and by their ortholog name identified on the right. 
The phosphopeptides were labelled if they are known conserved TOR targets as “known targets” and if they are unknown as “novel targets”. RbcL, MAPK, PP2C, PP2C, and SnRK2 were identified as putative novel conserved TOR targets. White boxes in the heatmap indicate that the phosphopeptides were not detected. Data represent means of fold changes log2 intensities of phosphopeptides (n = 3-4 biological replicates).
Reposted by Takehiro A. Ozawa
davidbiermann.bsky.social
Very excited to share that our paper "A RALF-brassinosteorid signaling circuit regulates Arabidopsis hypocotyl cell shape" is now published in @currentbiology.bsky.social !

Enjoy reading it!!

www.sciencedirect.com/science/arti...
Reposted by Takehiro A. Ozawa
structplantbio.bsky.social
Amazing work by the Santiago lab @unil.bsky.social. The malectin-LRR receptor kinase IGP1 senses cello-oligomers to alert the plant immune system & enhance disease resistance. Very nice discovery & mechanism.
www.biorxiv.org/content/10.1...
Reposted by Takehiro A. Ozawa
piotraz.bsky.social
🚀 We’re hiring a Postdoc!

Join our group in Poznan, Poland to study meiotic crossover recombination in plants 🌱 Highly motivated & enthusiastic candidates are welcome!
📅 Deadline: Nov 1, 2025

🔗 ibmib.web.amu.edu.pl/wp-content/uploads/2025/09/Postdoc_position-2025-Ziolkowskis-Lab.pdf
tkozawa.bsky.social
Great work by Wang et al. (2025) on how #PlantTOR dynamically regulates the translation of a chromatin-associated complex for growth (CACG) in #Arabidopsis, which represses the expression of stress-responsive genes to coordinate plant growth and stress tolerance 🌱⚖️🏜️.
🔗 www.nature.com/articles/s41...
Fig. 6 | Working model for the role of the nutrient–TOR–CACG axis in balancing plant growth and stress tolerance.

In the presence of adequate nutrients, TOR is active and promotes the translation of CACG mRNAs. The pyrimidine-rich motifs present in the 5′ UTRs of CACG mRNAs facilitate target selection by TOR. The CACG complex directly binds to numerous stress-responsive genes marked by histone acetylation, thereby repressing their transcription, which contributes to plant growth and development. 

Conversely, under nutrient-deficient conditions, TOR becomes inactive, leading to decreased translation of CACG mRNAs. This results in the alleviation of the repression of stress-response genes, causing reduced growth but increased stress tolerance. The nutrient–TOR–CACG axis is crucial for balancing plant growth with stress tolerance, promoting survival and reproductive success in variable environmental conditions. conditions. 

Image partially created in BioRender. He, X. Fig. 1 | Identification of a multi-subunit protein complex involved in nutrient-responsive growth.

a) Protein–protein interaction networks were generated from mass spectrometry data using Cytoscape. The edges show the interactions identified by AP–MS. CACG subunits are shown in pink, while COMPASS subunits and the INO80 complex (INO80-C) are shown in blue.
 
b) Interactions among CACG subunits as determined by Y2H and pull-down assays. Interactions revealed by Y2H assays are shown in yellow, those shown by pull-down assays in red and those confirmed by both methods in orange. Protein names in bold denote those analysed through pull-down assays.

c) Detection of a high-molecular-weight complex by gel filtration.
Proteins extracted from Flag-tagged GTE1, DP1, EMB1967 and CP2 transgenic plants were separated in a Superose 6 column (10/300 GL; GE Healthcare Life Sciences) and were detected by immunoblotting.

d) Immunoblot analysis of protein levels in the indicated Flag-tagged transgenic plants under different nutrient conditions. Twelve-day-old plants grown in solid MS medium were transferred to liquid MS, H2O or reduced concentrations of MS for 4 days. The actin protein level is shown as a loading control.

e) Fresh weight of wild-type plants irrigated with water or liquid MS medium (n = 24). The values are means ± s.d.

f) Immunoblot analysis of protein levels in the indicated Flag-tagged transgenic plants irrigated with water or liquid MS medium. The actin protein level is shown as a loading control.

g) Morphological phenotypes of 20-day-old wild-type and mutant plants (top) and 12-day-old wild-type and mutant plants (bottom). Scale bars, 1 cm.

The experiments in c, d and f were repeated independently twice and showed similar results. Modified Fig. 4 | The CACG complex suppresses the expression of stress-responsive genes to coordinate plant growth and stress tolerance.

g) Morphological phenotypes of wild-type plants and CACG mutants under control, drought stress and rehydration conditions. Twelve-day-old plants grown in solid MS medium were transferred to soil and subsequently subjected to drought treatment until the Col-0 plants began to show mortality. The phenotypes are shown before and three days after rehydration with MS. Plants watered with MS serve as the control.

h) The survival rate of wild-type plants and CACG mutants under drought stress conditions (n = 24). Each bar shows the mean ± s.d. from three independent biological replicates. P values were determined using two-tailed Student’s t-tests.

i) Immunoblot analysis of the protein levels of CACG components under the indicated conditions. TOR activity was monitored by the phosphorylation state of S6K. The actin protein level is shown as a loading control. The experiments were independently repeated twice and showed similar results.

j) Statistical analysis of pS6K protein levels. Actin was used as a loading control. Each black dot represents an independent biological replicate.
Reposted by Takehiro A. Ozawa
mplantpcom.bsky.social
The marine diatom Phaeodactylum tricornutum as a versatile bioproduction chassis: Current progress, challenges and perspectives #review #PlantCommunications cell.com/plant-commun...
Reposted by Takehiro A. Ozawa
mplantpcom.bsky.social
Multi-dimensional epigenomic dynamics converge on H3K4 regulation of low CO2 adaptation in Nannochloropsis oceanica #research #PlantCommunications cell.com/plant-commun...
Reposted by Takehiro A. Ozawa
cellarchlab.com
Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on!

#TeamTomo #PlantScience 🧪 🧶🧬 🌾
elifesciences.org/articles/105...
1/🧵
Reposted by Takehiro A. Ozawa
kleinevehnlab.bsky.social
1/ 🌱 When plants face stress, they often suppress growth to save resources.
In our new Science Advances paper, we reveal how: plants repurpose their ER quality control machinery (ERAD) to regulate auxin compartmentalization and thus growth.
www.science.org/doi/10.1126/...
ERAD machinery controls the conditional turnover of PIN-LIKES in plants
Plant ERAD machinery governs the turnover of auxin transporters involved in developmental and stress responses.
www.science.org
Reposted by Takehiro A. Ozawa
daanweits.bsky.social
Coming to you live from #ISPLORE2025JP Fresh preprint from my lab showing that leaves progressively oxygenate and how this is important for their morphogenesis.Thanks to our collaborators from @Fra_LicO2si lab. #plantscience
www.biorxiv.org/content/10.1...