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Reposted by Edda Schulz

Max Planck Institute for Molecular Genetics @molgen.mpg.de · 6h

New paper! In female mammals, one X-chromosome is inactive in each cell. In some cats, this causes typical color patterns, depending on which X is active and the color it codes for. The @eddaschulz.bsky.social lab found a new regulatory principle behind X-inactivation. 1/2 Note: AI generated image

Calico cat on a neutral background. Image is AI generated with Adobe Firefly.

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Reposted by Edda Schulz

Rob Klose @robklose.bsky.social · 9h

Wonderful opportunity to work with Martin Howard and my group trying to understand how chromatin influences gene transcription and expression. Integration of theory, modelling, and experimentation. Come and join the team!
Please repost!

jobs.jic.ac.uk/Details.asp?...

John Innes Centre

jobs.jic.ac.uk

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Reposted by Edda Schulz

Nature Structural & Molecular Biology @natsmb.nature.com · 2d

New online: Reporter CRISPR screens decipher cis-regulatory and trans-regulatory principles at the Xist locus

Reporter CRISPR screens decipher cis-regulatory and trans-regulatory principles at the Xist locus

Nature Structural & Molecular Biology, Published online: 06 October 2025; doi:10.1038/s41594-025-01686-3Here Schwämmle et al. develop CRISPR reporter screens to map transcription-factor-regulatory element interactions at the Xist locus, revealing a two-step mechanism integrating developmental and X-dosage signals to initiate X-chromosome inactivation.

go.nature.com

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Edda Schulz @eddaschulz.bsky.social · 1d

Thank you, Max!

Reposted by Edda Schulz

Till Schwämmle @tschwammle.bsky.social · 2d

Happy to share the 2nd part of my PhD @natsmb.nature.com!

Using CRISPR screens, we uncover the TF-RE wiring underlying Xist regulation. Since the preprint, we've added a titration of Xist, showing that XCI is directly dependent on Xist levels.

Check the Bluetorial by @eddaschulz.bsky.social 👇

Edda Schulz @eddaschulz.bsky.social · 2d

⚠️ Paper alert: Using a novel CRISPR screening approach, we mapped the entire regulatory network controlling Xist—key for X-chromosome inactivation.
👉 We discover how sex and development signals are decoded at a single gene locus.
www.nature.com/articles/s41...
👇 Bluetorial

Reporter CRISPR screens decipher cis-regulatory and trans-regulatory principles at the Xist locus - Nature Structural & Molecular Biology

Here Schwämmle et al. develop CRISPR reporter screens to map transcription-factor-regulatory element interactions at the Xist locus, revealing a two-step mechanism integrating developmental and X-dosage signals to initiate X-chromosome inactivation.

www.nature.com

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Reposted by Edda Schulz

Gemma Noviello @gemnov.bsky.social · 2d

Happy to see this insightful and rigorous work published in its final form! Congrats to all authors and in particular to super hard-working first author @tschwammle.bsky.social and to @eddaschulz.bsky.social for the brilliant guidance.

Edda Schulz @eddaschulz.bsky.social · 2d

⚠️ Paper alert: Using a novel CRISPR screening approach, we mapped the entire regulatory network controlling Xist—key for X-chromosome inactivation.
👉 We discover how sex and development signals are decoded at a single gene locus.
www.nature.com/articles/s41...
👇 Bluetorial

Reporter CRISPR screens decipher cis-regulatory and trans-regulatory principles at the Xist locus - Nature Structural & Molecular Biology

Here Schwämmle et al. develop CRISPR reporter screens to map transcription-factor-regulatory element interactions at the Xist locus, revealing a two-step mechanism integrating developmental and X-dosage signals to initiate X-chromosome inactivation.

www.nature.com

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Edda Schulz @eddaschulz.bsky.social · 2d

Thanks, Raquel!

Edda Schulz @eddaschulz.bsky.social · 2d

🥂 Congratulation to first author Till Schwämmle @tschwammle.bsky.social and all co-authors! @gemnov.bsky.social @nellykanata.bsky.social @jjfroehlich.bsky.social @melissabothe.bsky.social @alexandrammar.bsky.social @aybuge-altay.bsky.social @molgen.mpg.de

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Edda Schulz @eddaschulz.bsky.social · 2d

🎯 Why It Matters: High Xist Levels are required for silencing the entire chromosome.
🎛️ Using CasTuner and enhancer deletions we show that Xist expression and silencing efficiency are dose-dependent 📈: reduced Xist RNA = impaired X-chromosome silencing

$Violin plots showing the fraction of X-linked reads coming from the inactive X as a function of Xist expression level. The plot shows that lower Xist expression results in weaker X-chromosome silencing.$

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Edda Schulz @eddaschulz.bsky.social · 2d

🔹 Phase 2 (High-Level Boost): Late TFs activate distal enhancers
→ They drive high Xist RNA levels needed for robust silencing.

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Edda Schulz @eddaschulz.bsky.social · 2d

The Xist switch has two distinct phases:
🔹 Phase 1 (Binary ON/OFF): Early TFs target proximal regulatory elements
→ They sense X-chromosome number (XX vs XY) to trigger initial Xist upregulation—ensuring female-specific activation.

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Edda Schulz @eddaschulz.bsky.social · 2d

A cell with two X chromosomes must silence one to survive.
This is done by the Xist gene, a master regulator that coats one X in RNA.
But how does Xist KNOW when to turn on—and only in females?
🔬 Our new study reveals a two-step regulatory logic that decodes sex and development.

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Edda Schulz @eddaschulz.bsky.social · 2d

⚠️ Paper alert: Using a novel CRISPR screening approach, we mapped the entire regulatory network controlling Xist—key for X-chromosome inactivation.
👉 We discover how sex and development signals are decoded at a single gene locus.
www.nature.com/articles/s41...
👇 Bluetorial

Reporter CRISPR screens decipher cis-regulatory and trans-regulatory principles at the Xist locus - Nature Structural & Molecular Biology

Here Schwämmle et al. develop CRISPR reporter screens to map transcription-factor-regulatory element interactions at the Xist locus, revealing a two-step mechanism integrating developmental and X-dosage signals to initiate X-chromosome inactivation.

www.nature.com

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Edda Schulz @eddaschulz.bsky.social · 6d

Congratulations, Raquel!

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Edda Schulz @eddaschulz.bsky.social · 19d

congratulations, Jan and team! Sounds really exciting!

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Edda Schulz @eddaschulz.bsky.social · Sep 6

Wow, that's great! I'm really happy to hear that!

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Edda Schulz @eddaschulz.bsky.social · Aug 20

Congratulations, Andi and team!

Reposted by Edda Schulz

Sandra Hake @hake-chromatin.bsky.social · Aug 19

Congratulations, Dr. Kalita!!!

Gesellschaft für Genetik @gfgenetik.bsky.social · Aug 19

Dr. Agata Kalita wins the prestigious GfG Elisabeth-Gateff Prize 2025 for her amazing work on sex chromosome dosage compensation in insects!
Dr. Kalita will receive the award at the upcoming GfG Symposium “Epigenetics & Chromatin” on September 26th, 2025 (express.converia.de/frontend/ind...).

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Reposted by Edda Schulz

Sandra Iden @sandraiden.bsky.social · Aug 17

Cell biology enthusiasts: We look forward to the #DGZ meeting in Heidelberg in October to celebrate 50 years of @dgz.bsky.social! 4 keynote speakers, 12 DGZ workgroups showcasing the latest in their fields & DGZ awardees 2025 will present. Register by Sept 15. www.zellbiologie.de/dgz-internat...

DGZ International Meeting 2025 › DGZ

...

www.zellbiologie.de

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Reposted by Edda Schulz

Max Planck Institute for Molecular Genetics @molgen.mpg.de · Aug 1

🧬 New study from the Kinkley Lab! 🧬 In collaboration with the Vingron and Hnisz labs at the MPIMG the team has gained new insights how the epigenetic reader PHF13 regulates it´s affinity to bind to chromatin.
Read more: www.molgen.mpg.de/2025-07-28-o...
Congrats to first author Francesca Rossi!

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Edda Schulz @eddaschulz.bsky.social · Jul 16

Great Paper! Congrats, Henrik and team!

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Reposted by Edda Schulz

Ingrid Pelaez Conde @ingridpelaez.bsky.social · Jul 3

I'm really happy to see the work from my Master's thesis published! 🎉

Shoutout to everyone who helped make this happen — especially @albaperez.bsky.social for the amazing mentorship and Alfred for the opportunity to work on this cool project!

Check it out 👀

CortesMalaria Lab @cortesmalaria.bsky.social · Jun 3

📣Rush to read our latest paper!! journals.plos.org/plospathogen.... You'll learn why #heterochromatin is formed and maintained in specific regions of the Plasmodium falciparum genome and not others 🧵⬇️

Heterochromatin de novo formation and maintenance in Plasmodium falciparum

Author summary Proper regulation of gene expression is crucial for the functioning of all organisms. One key mechanism involves modifications in the structure of chromatin, the DNA and proteins comple...

journals.plos.org

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Reposted by Edda Schulz

Mike Robson @drmrobson.bsky.social · Jul 8

Calling all aspiring Postdocs! One extra week to apply to join our exciting HFSP-funded project to uncover how chromatin moves to function.

Deadline July 15th.

www.mdc-berlin.de/career/jobs/...

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Edda Schulz @eddaschulz.bsky.social · Jul 3

Congratulations, Ingrid!

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Edda Schulz @eddaschulz.bsky.social · Jul 2

Congratulations, Ina! Very well deserved!

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