David Turner
@gastruloids.bsky.social
150 followers 40 following 6 posts
Stem cell and developmental biologist at the University of Liverpool
Posts Media Videos Starter Packs
Reposted by David Turner
jamesbriscoe.bsky.social
James Briscoe @jamesbriscoe.bsky.social · 3d
This year's Crick @crick.ac.uk PhD programme is open for applications

- £27,715 tax-free student stipend per year for 4 years
- Tuition fees paid
- Student visa fee & health surcharges covered

Deadline 5 Nov

We have a project available. For all the details visit:

www.crick.ac.uk/careers-stud...
Briscoe Lab | Cellular Dynamics and Pattern Formation in Vertebrate Development
www.crick.ac.uk
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Reposted by David Turner
dev-journal.bsky.social
Development @dev-journal.bsky.social · Jul 11
New insights directly link WT1 and smooth muscle cell lineages

Read this Research Highlight showcasing work from Suad Alsukari, Huei Teng Ng, @gastruloids.bsky.social, Bettina Wilm @bwilm.bsky.social and colleagues:
journals.biologists.com/dev/article/...
Figure 4 WT1 is expressed in the primitive streak, emerging mesoderm, neuromesodermal progenitor cells and LPM in E8.5 mouse embryos
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Reposted by David Turner
bwilm.bsky.social
Bettina Wilm @bwilm.bsky.social · Jul 9
Very happy to announce that this article is now published @dev-journal.bsky.social @biologists.bsky.social
journals.biologists.com/dev/article/...
With Suad Alsukari and Huei Teng Ng and @gastruloids.bsky.social
Dynamic WT1 expression during gastrulation specifies peritoneal smooth muscle fate independently of mesothelial fate
Highlighted Article: Genetic lineage tracing and expression analyses reveal insights into the developmental lineage of smooth muscle cells in the intestine and mesentery, specified by WT1 expression d...
journals.biologists.com
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Reposted by David Turner
amartinezarias.bsky.social
Alfonso Martinez Arias @amartinezarias.bsky.social · Jul 4
An englishman, down and out in Paris…..trying to read the news and laughing....at a french joke?
@gastruloids.bsky.social‬
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David Turner @gastruloids.bsky.social · Jun 30
Huge thanks to‬ @cci-liv.bsky.social for the amazing microscopes and technical support throughout this work!
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Reposted by David Turner
cci-liv.bsky.social
CCI Uni of Liverpool @cci-liv.bsky.social · May 13
Look at this amount of work that has gone into the analysis of these beautiful (yes, we are biased) images of rods and rings. Now you can automatically classify these structures which is rather tedious to do manually. 🔬📷🖼️💻📄 bmcbiol.biomedcentral.com/articles/10....
Domain-specific AI segmentation of IMPDH2 rod/ring structures in mouse embryonic stem cells - BMC Biology
Background Inosine monophosphate dehydrogenase 2 (IMPDH2) is an enzyme that catalyses the rate-limiting step of guanine nucleotides. In mouse embryonic stem cells (ESCs), IMPDH2 forms large multi-prot...
bmcbiol.biomedcentral.com
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Reposted by David Turner
makaefer.bsky.social
Marie Held @makaefer.bsky.social · May 13
I cannot help but think of these rod-rings as oh so pretty. amazing work by some of our @cci-liv.bsky.social users.
cci-liv.bsky.social
CCI Uni of Liverpool @cci-liv.bsky.social · May 13
Look at this amount of work that has gone into the analysis of these beautiful (yes, we are biased) images of rods and rings. Now you can automatically classify these structures which is rather tedious to do manually. 🔬📷🖼️💻📄 bmcbiol.biomedcentral.com/articles/10....
Domain-specific AI segmentation of IMPDH2 rod/ring structures in mouse embryonic stem cells - BMC Biology
Background Inosine monophosphate dehydrogenase 2 (IMPDH2) is an enzyme that catalyses the rate-limiting step of guanine nucleotides. In mouse embryonic stem cells (ESCs), IMPDH2 forms large multi-prot...
bmcbiol.biomedcentral.com
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David Turner @gastruloids.bsky.social · Jun 23
Great preprint out from the MicroAge team in
@livuni-ilcams.bsky.social! www.biorxiv.org/content/10.1...
www.biorxiv.org
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Reposted by David Turner
amartinezarias.bsky.social
Alfonso Martinez Arias @amartinezarias.bsky.social · Jun 23
Excellent work supporting #modularity of the mammalian #embryo, a role for #hypoxia & how development exploits these features. It shows what we can learn from assemblies of #gastruloids @ spatio-temporal coordination of the modules in AP organization. Putting modules together, extremely informative
bulutkarslioglu.bsky.social
Aydan Bulut-Karslioglu @bulutkarslioglu.bsky.social · Jun 23
⚠️ I am really excited to share the work of Anastasios Balaskas, an excellent PhD candidate in the lab, with the wider world. Tasos made a significant advance: generating a stem cell-based embryo model that contains both posterior and anterior neural tissues of the late-stage gastrulating embryo.
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Reposted by David Turner
amartinezarias.bsky.social
Alfonso Martinez Arias @amartinezarias.bsky.social · Jun 23
The modularity of the mammalian #embryo in display. Controlled assembly of classic #gastruloids and uncommitted precursors in #hypoxia, patterns the AP axis
www.biorxiv.org/content/10.1...
Beautiful & insightful from @bulutkarslioglu.bsky.social‬ group. Building models to learn and learning.
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Reposted by David Turner
bwilm.bsky.social
Bettina Wilm @bwilm.bsky.social · Jun 17
This was a fabulous collaboration! Thanks @gastruloids.bsky.social and to Suad and Huei who worked incredibly hard to make it happen!
David Turner @gastruloids.bsky.social · Jun 17
Great to see our article published in @dev-journal.bsky.social! Wonderfull collaboration with @bwilm.bsky.social to understand how Wt1 guides cell fate in the early gastrulating embryo! journals.biologists.com/dev/article/...
Dynamic WT1 expression during gastrulation specifies peritoneal smooth muscle fate independently from mesothelial fate
The Wilms’ Tumour protein (WT1) was previously linked to the mesothelial and vascular smooth muscle cell (vSMC) lineage in the mouse intestine, with evidence suggesting that intestinal vSMCs arise fro...
journals.biologists.com
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Reposted by David Turner
amartinezarias.bsky.social
Alfonso Martinez Arias @amartinezarias.bsky.social · Jun 17
Also, an interesting reflection from Bob Edwards on the contrast between Research Institutes and Universities. Having spent my academic life in the latter, I can relate. From "A matter of life" a first hand account of the path to IVF that I suggest as a read for those interested in human #DevBio
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David Turner @gastruloids.bsky.social · Jun 17
Great to see our article published in @dev-journal.bsky.social! Wonderfull collaboration with @bwilm.bsky.social to understand how Wt1 guides cell fate in the early gastrulating embryo! journals.biologists.com/dev/article/...
Dynamic WT1 expression during gastrulation specifies peritoneal smooth muscle fate independently from mesothelial fate
The Wilms’ Tumour protein (WT1) was previously linked to the mesothelial and vascular smooth muscle cell (vSMC) lineage in the mouse intestine, with evidence suggesting that intestinal vSMCs arise fro...
journals.biologists.com
1
Reposted by David Turner
choijunhong.bsky.social
Junhong Choi @choijunhong.bsky.social · May 27
We previously showed that DNA Typewriter can record high-resolution lineage trees in cultured cells (HEK293T). www.nature.com/articles/s41... But could we use it to study how clonal memory shapes cell fate decisions during development? (2/13)
A time-resolved, multi-symbol molecular recorder via sequential genome editing - Nature
A DNA memory device, DNA Typewriter, uses sequential prime editing to record the order of multiple cellular events.
www.nature.com
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Reposted by David Turner
choijunhong.bsky.social
Junhong Choi @choijunhong.bsky.social · May 27
New preprint with the Shendure lab @jshendure.bsky.social ! We apply the DNA Typewriter lineage recorder to mouse 3D gastruloids, a powerful model of early mammalian development. Led by Sam Regalado and CX Qiu @cxqiu.bsky.social : www.biorxiv.org/content/10.1... (1/13)
Lineage recording in monoclonal gastruloids reveals heritable modes of early development
Mammalian stem cells possess a remarkable capacity for self-organization, a property that underlies increasingly sophisticated in vitro models of early development. However, even under carefully contr...
www.biorxiv.org
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Reposted by David Turner
dias-andre.bsky.social
André Dias @dias-andre.bsky.social · May 16
We've just updated our manuscript to a more 'publishable' version!
www.biorxiv.org/content/10.1...
New details about the role of Wnt in suppressing neural differentiation and fostering a posterior epiblast state, and the temporal effect of Nodal signalling in driving mesendodermal fates
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David Turner @gastruloids.bsky.social · May 12
Our new paper just arrived! Fun collaborative effort led by Sam Ball and Meagan Hennessy to develop an AI tool to segment and classify #IMPDH2 rod-rings in mouse ESCs doi.org/10.1186/s129...
Domain-specific AI segmentation of IMPDH2 rod/ring structures in mouse embryonic stem cells - BMC Biology
Background Inosine monophosphate dehydrogenase 2 (IMPDH2) is an enzyme that catalyses the rate-limiting step of guanine nucleotides. In mouse embryonic stem cells (ESCs), IMPDH2 forms large multi-prot...
doi.org
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Reposted by David Turner
biologists.bsky.social
The Company of Biologists @biologists.bsky.social · Mar 27
Great fun at last night's Gala Dinner at the stunning
St Georges Hall.
#biologists100
Gala dinner of the Biologists @ 100 conference at St Georges Hall, Liverpool.
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Reposted by David Turner
amartinezarias.bsky.social
Alfonso Martinez Arias @amartinezarias.bsky.social · Mar 28
Right after the spanish edition of #TheMasterBuilder comes the Portuguese one, thanks to www.temasedebates.pt
Have a look for a cell’s eye view of Biology in contrast with the widespread gene’s eye.
#CellsRUs #NotInTheGenes
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Reposted by David Turner
amartinezarias.bsky.social
Alfonso Martinez Arias @amartinezarias.bsky.social · Mar 21
Wonderful evening in #Barcelona w/ friends sharing memories, science and hope in these troubled times.
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Reposted by David Turner
amartinezarias.bsky.social
Alfonso Martinez Arias @amartinezarias.bsky.social · Mar 22
In the wake of Barcelona #Gastruloid meeting, we can say w/ some confidence that this is a system w/ potential in several areas of biomedical research: models, rather than replicas, are useful &also teach. For details
linkinghub.elsevier.com/retrieve/pii...
onlinelibrary.wiley.com/doi/10.1002/...
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Reposted by David Turner
bwilm.bsky.social
Bettina Wilm @bwilm.bsky.social · Mar 22
Delighted to announce our preprint on modelling of the peritoneum in endometriosis research, by Dimas Rahmana, with Dharani Hapangama's group, Liverpool Women's Hospital, with essential contribution from Chris Hill
www.biorxiv.org/content/10.1...
@livunihls.bsky.social @liverpooluni.bsky.social
Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis
Peritoneal endometriosis causes pelvic pain and infertility, but the underlying mechanisms related to these symptoms are not fully understood. Endometriosis diagnosis is typically delayed; thus, patie...
www.biorxiv.org
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Reposted by David Turner
joshifrenster.bsky.social
Joshua Frenster @joshifrenster.bsky.social · Mar 20
First day of our Gastruloid Meeting in Barcelona kicking off with amazing talks and discussion and a room filled with gastruloid enthusiasts! #gastruloids #conference
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Reposted by David Turner
dias-andre.bsky.social
André Dias @dias-andre.bsky.social · Mar 17
Fun collaboration! All the nitty-gritty about N2B27 and gastruloids. Kudos to all the authors, particularly Tina Balayo (¡que va de primero!)
David Turner @gastruloids.bsky.social · Mar 17
New Gastruloid preprint just landed... nice collaboration between our lab, @dias-andre.bsky.social, and @amartinezarias.bsky.social www.biorxiv.org/content/10.1...
N2B27 media formulations influence gastruloid development
Gastruloids are 3D aggregates of pluripotent stem cells grown in suspension culture that mimic many aspects of gastrulation and early axial elongation. The N2B27 basal medium in which mouse gastruloid...
www.biorxiv.org
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David Turner @gastruloids.bsky.social · Mar 17
New Gastruloid preprint just landed... nice collaboration between our lab, @dias-andre.bsky.social, and @amartinezarias.bsky.social www.biorxiv.org/content/10.1...
N2B27 media formulations influence gastruloid development
Gastruloids are 3D aggregates of pluripotent stem cells grown in suspension culture that mimic many aspects of gastrulation and early axial elongation. The N2B27 basal medium in which mouse gastruloid...
www.biorxiv.org
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