chem bio girl
@ih8kras.bsky.social
former chem bio undergrad, now in grad school; just a girl.
Reposted by chem bio girl
Organelles do NOT have a single uniform pH.
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
Organelles harbour pH gradients https://www.biorxiv.org/content/10.64898/2025.12.12.694065v1
December 17, 2025 at 12:46 AM
Organelles do NOT have a single uniform pH.
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n
Reposted by chem bio girl
Combining expansion microscopy with super-resolution radial fluctuations captures the morphology of single proteins go.nature.com/3Y1FD8M
rdcu.be/eI29D
rdcu.be/eI29D
One-step nanoscale expansion microscopy reveals individual protein shapes - Nature Biotechnology
Combining expansion microscopy with super-resolution radial fluctuations captures the morphology of single proteins.
go.nature.com
October 3, 2025 at 1:45 AM
Combining expansion microscopy with super-resolution radial fluctuations captures the morphology of single proteins go.nature.com/3Y1FD8M
rdcu.be/eI29D
rdcu.be/eI29D
Reposted by chem bio girl
It's #ChemNobel season again!
Surely this year should go to the most important chemical breakthrough of our lifetimes: DNA synthesis/Marvin Caruthers 🧬
Phosphoramidite DNA synthesis is the bedrock of modern biology and enabled the development of therapies for untreatable diseases
#chemsky 🧪
Surely this year should go to the most important chemical breakthrough of our lifetimes: DNA synthesis/Marvin Caruthers 🧬
Phosphoramidite DNA synthesis is the bedrock of modern biology and enabled the development of therapies for untreatable diseases
#chemsky 🧪
It's Chem Nobel Prize season, so your reminder that DNA synthesis/Marvin Caruthers needs to win! chemsky 🧪
Without DNA synthesis there is NO *PCR*, *sequencing*, *cloning*, *CRISPR*, *siRNA*, *synthetic biology*, *mRNA vaccines*, *nucleic acid therapeutics*, *Nanotech*
What more am I missing?
Without DNA synthesis there is NO *PCR*, *sequencing*, *cloning*, *CRISPR*, *siRNA*, *synthetic biology*, *mRNA vaccines*, *nucleic acid therapeutics*, *Nanotech*
What more am I missing?
October 1, 2025 at 9:13 AM
It's #ChemNobel season again!
Surely this year should go to the most important chemical breakthrough of our lifetimes: DNA synthesis/Marvin Caruthers 🧬
Phosphoramidite DNA synthesis is the bedrock of modern biology and enabled the development of therapies for untreatable diseases
#chemsky 🧪
Surely this year should go to the most important chemical breakthrough of our lifetimes: DNA synthesis/Marvin Caruthers 🧬
Phosphoramidite DNA synthesis is the bedrock of modern biology and enabled the development of therapies for untreatable diseases
#chemsky 🧪
Reposted by chem bio girl
So screaming at cells if they don't express my favorite protein should work, no? 😂🧪
July 12, 2025 at 1:21 PM
So screaming at cells if they don't express my favorite protein should work, no? 😂🧪
Reposted by chem bio girl
If u ever had to depict proteins as schematics in papers, u know the dilemma: how to create a pretty “blob”?
If structure ends up big & central, u’ll render it with PyMol et al, but for a truly schematic depiction u want a biorender-style icon, but for YOUR protein.
Think I’ve finally found a way.
If structure ends up big & central, u’ll render it with PyMol et al, but for a truly schematic depiction u want a biorender-style icon, but for YOUR protein.
Think I’ve finally found a way.
May 3, 2025 at 12:40 PM
If u ever had to depict proteins as schematics in papers, u know the dilemma: how to create a pretty “blob”?
If structure ends up big & central, u’ll render it with PyMol et al, but for a truly schematic depiction u want a biorender-style icon, but for YOUR protein.
Think I’ve finally found a way.
If structure ends up big & central, u’ll render it with PyMol et al, but for a truly schematic depiction u want a biorender-style icon, but for YOUR protein.
Think I’ve finally found a way.
Reposted by chem bio girl
Cool application of in vivo bioorthogonal chemistry from Biogen, imaging biodistribution of ASOs in the brain 🤯
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
PET imaging of antisense oligonucleotide distribution in rat and nonhuman primate brains using click chemistry
Intrathecally dosed antisense oligonucleotide distribution can be imaged in rat and nonhuman primate brains using a PET tracer and click chemistry.
www.science.org
May 8, 2025 at 10:12 PM
Cool application of in vivo bioorthogonal chemistry from Biogen, imaging biodistribution of ASOs in the brain 🤯
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by chem bio girl
We’re thrilled to be featured on the May cover of Nature Microbiology... And our Research Briefing is out now—check it out, it’s 🔥! www.nature.com/articles/s41...
May 6, 2025 at 7:18 PM
We’re thrilled to be featured on the May cover of Nature Microbiology... And our Research Briefing is out now—check it out, it’s 🔥! www.nature.com/articles/s41...
Reposted by chem bio girl
ATLAS, a rationally designed transsynaptic tracer is out today! Plasmids will soon be available on
@addgene.bsky.social
addgene nature.com/articles/s41...
Many thanks to first authors Jackie Rivera and Haoyang Huang, and to collaborators @blsabatini.bsky.social @beherring.bsky.social
@addgene.bsky.social
addgene nature.com/articles/s41...
Many thanks to first authors Jackie Rivera and Haoyang Huang, and to collaborators @blsabatini.bsky.social @beherring.bsky.social
ATLAS: a rationally designed anterograde transsynaptic tracer - Nature Methods
ATLAS is a tool for circuit tracing, demonstrated here in rodents. It allows anterograde transsynaptic tracing, starting from genetically defined neurons.
nature.com
May 1, 2025 at 2:56 PM
ATLAS, a rationally designed transsynaptic tracer is out today! Plasmids will soon be available on
@addgene.bsky.social
addgene nature.com/articles/s41...
Many thanks to first authors Jackie Rivera and Haoyang Huang, and to collaborators @blsabatini.bsky.social @beherring.bsky.social
@addgene.bsky.social
addgene nature.com/articles/s41...
Many thanks to first authors Jackie Rivera and Haoyang Huang, and to collaborators @blsabatini.bsky.social @beherring.bsky.social
Reposted by chem bio girl
Excited to see this out in @science.org today!! www.science.org/doi/10.1126/...
May 1, 2025 at 6:17 PM
Excited to see this out in @science.org today!! www.science.org/doi/10.1126/...
Reposted by chem bio girl
Reposted by chem bio girl
Tetanus on demand! (not for bioweapons, but for neuroscience research)
Tetanus toxin induces paralysis by using a protease to cleave VAMP2, a protein required for synaptic vesicle fusion and neurotransmitter release.
To harness this toxicity and use it as a beneficial tool for neuroscience,
Tetanus toxin induces paralysis by using a protease to cleave VAMP2, a protein required for synaptic vesicle fusion and neurotransmitter release.
To harness this toxicity and use it as a beneficial tool for neuroscience,
January 30, 2025 at 2:30 PM
Tetanus on demand! (not for bioweapons, but for neuroscience research)
Tetanus toxin induces paralysis by using a protease to cleave VAMP2, a protein required for synaptic vesicle fusion and neurotransmitter release.
To harness this toxicity and use it as a beneficial tool for neuroscience,
Tetanus toxin induces paralysis by using a protease to cleave VAMP2, a protein required for synaptic vesicle fusion and neurotransmitter release.
To harness this toxicity and use it as a beneficial tool for neuroscience,
Reposted by chem bio girl
Genetically Encoded Fluorogenic DNA Aptamers for Imaging Metabolite in Living Cells
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Genetically Encoded Fluorogenic DNA Aptamers for Imaging Metabolite in Living Cells
Genetically encoded fluorescent protein and fluorogenic RNA sensors are indispensable tools for imaging biomolecules in cells. To expand the toolboxes and improve the generalizability and stability of...
pubs.acs.org
January 14, 2025 at 9:34 PM
Genetically Encoded Fluorogenic DNA Aptamers for Imaging Metabolite in Living Cells
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Reposted by chem bio girl
Reposted by chem bio girl
I’m trying this when I get back to work. Looks pretty!
December 2, 2024 at 11:32 PM
I’m trying this when I get back to work. Looks pretty!
Reposted by chem bio girl
ok I swear I didn't come to these bluest of skies just to hawk work stuff; BUT these tRNA ornaments are finally up on our website, and I can personally confirm that they are really satisfying to play with! #CyberMonday #SciArt 🧪
shop.3dmoleculardesigns.com/products/202...
shop.3dmoleculardesigns.com/products/202...
2024 Holiday Ornament: tRNA
tRNA folds into this beautiful 3D structure before carrying amino acids to ribosomes for protein synthesis. It even forms A-U and G-C base pairs! This elegant structure differs greatly from the clover...
shop.3dmoleculardesigns.com
December 2, 2024 at 8:27 PM
ok I swear I didn't come to these bluest of skies just to hawk work stuff; BUT these tRNA ornaments are finally up on our website, and I can personally confirm that they are really satisfying to play with! #CyberMonday #SciArt 🧪
shop.3dmoleculardesigns.com/products/202...
shop.3dmoleculardesigns.com/products/202...
Reposted by chem bio girl
Come with me as I troubleshoot this very high GC PCR toward clonable homology arms in an actinobacteria. Testing DMSO, Betaine 1M, and Propylene Glycol to see if we get amplification. Arms are 1000bp. Full length with arms and gene is 2800bp. Let's go!
🧵 1/n
🧵 1/n
December 11, 2024 at 5:26 PM
Come with me as I troubleshoot this very high GC PCR toward clonable homology arms in an actinobacteria. Testing DMSO, Betaine 1M, and Propylene Glycol to see if we get amplification. Arms are 1000bp. Full length with arms and gene is 2800bp. Let's go!
🧵 1/n
🧵 1/n
Reposted by chem bio girl
Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from gene expression and real-time fluorescence to modulation..
December 4, 2024 at 4:05 PM
Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from gene expression and real-time fluorescence to modulation..