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Reposted by KJ

Lorne Proteins @lorneproteins.bsky.social · 15d

📢 𝙰𝚋𝚜𝚝𝚛𝚊𝚌𝚝 𝚂𝚞𝚋𝚖𝚒𝚜𝚜𝚒𝚘𝚗𝚜 𝙲𝚕𝚘𝚜𝚒𝚗𝚐 𝚂𝚘𝚘𝚗!!

𝙳𝚘𝚗’𝚝 𝚖𝚒𝚜𝚜 𝚢𝚘𝚞𝚛 𝚌𝚑𝚊𝚗𝚌𝚎 𝚝𝚘 𝚜𝚑𝚊𝚛𝚎 𝚢𝚘𝚞𝚛 𝚛𝚎𝚜𝚎𝚊𝚛𝚌𝚑 𝚊𝚝 𝚝𝚑𝚎 𝙻𝚘𝚛𝚗𝚎 𝙿𝚛𝚘𝚝𝚎𝚒𝚗 𝙲𝚘𝚗𝚏𝚎𝚛𝚎𝚗𝚌𝚎 𝟸𝟶𝟸𝟼. 𝙰𝚋𝚜𝚝𝚛𝚊𝚌𝚝 𝚜𝚞𝚋𝚖𝚒𝚜𝚜𝚒𝚘𝚗𝚜 𝚌𝚕𝚘𝚜𝚎 𝟸𝟺𝚝𝚑 𝙾𝚌𝚝𝚘𝚋𝚎𝚛 – 𝚜𝚑𝚘𝚠𝚌𝚊𝚜𝚎 𝚢𝚘𝚞𝚛 𝚠𝚘𝚛𝚔 𝚝𝚘 𝚝𝚑𝚎 𝚐𝚕𝚘𝚋𝚊𝚕 𝚙𝚛𝚘𝚝𝚎𝚒𝚗 𝚜𝚌𝚒𝚎𝚗𝚌𝚎 𝚌𝚘𝚖𝚖𝚞𝚗𝚒𝚝𝚢 𝚒𝚗 𝚐𝚕𝚘𝚛𝚒𝚘𝚞𝚜 𝙻𝚘𝚛𝚗𝚎! 🏖️ 🏄‍♀️ 🏊

👉 𝚂𝚞𝚋𝚖𝚒𝚝:
www.lorneproteins.org/submissions

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eLife @elife.bsky.social · 8d

Cryogenic electron microscopy has been used to determine the detailed structure of an intermediate state called a “D-loop” that forms when strands of DNA are exchanged during homologous recombination.
buff.ly/pZZfIso

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Augustinas Silale @augustsilale.bsky.social · 8d

Our paper on the Bacteroidota BAM complex is out in @natmicrobiol.nature.com! With @madejmar.bsky.social

We found that BAM in Bacteroides and Porphyromonas gingivalis has a distinct architecture from BAM in Proteobacteria.

doi.org/10.1038/s415...

Structure of a distinct β-barrel assembly machinery complex in the Bacteroidota - Nature Microbiology

Structural and functional characterization of the β-barrel assembly machinery complex in Bacteroidota reveals a distinct, seven-component complex with a large extracellular domain that may enable β-barrel–surface lipoprotein complex assembly.

doi.org

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Kexin Zhang @kekexinz.bsky.social · 22d

🚀 Check out our new preprint on extending the scope of single-particle cryo-EM with 2D template matching!
Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching is now available on bioRxiv!
www.biorxiv.org/content/10.1...

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Herve Celia @hervecelia.bsky.social · Sep 4

www.sciencedirect.com/science/arti...

Characterization of the OMP biogenesis machinery in Fusobacterium nucleatum

F. nucleatum is a Gram-negative bacteria that causes oral infections and is linked to colorectal cancer. Pathogenicity relies on a type of β-barrel ou…

www.sciencedirect.com

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Herve Celia @hervecelia.bsky.social · Sep 4

www.sciencedirect.com/science/arti...

Structural insights into outer membrane protein biogenesis in pathogenic Neisseria

N. gonorrhoeae (Ngo) causes the sexually transmitted infection gonorrhea with ∼106 million infections worldwide annually. Ngo infections can result in…

www.sciencedirect.com

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The EMBO Journal @embojournal.org · Sep 4

Bacterial cell widening alters periplasmic size and activates envelope stress responses
Kerwyn Huang, @typaslab.bsky.social et al find that E. coli outer-membrane protein RcsF senses reduced periplasm thickness due to increased cell width, & activates Rcs signaling
www.embopress.org/doi/full/10....

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MACSYS @macsys.bsky.social · May 19

🚨 Exciting PhD Opportunities with MACSYS! The MACSYS team at Monash University is offering multiple fully funded #PhD scholarships for students eager to explore the cutting edge of computational biology, microbiology, & systems modelling.
👉More info/apply: macsys.org/monash-phd-s...

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Martin Smith @martinalexsmith.bsky.social · Aug 25

RNA is far more than a messenger.

Its structures regulate, evolve, and catalyze — carrying out functions DNA alone cannot.

But predicting #RNA structure from sequence is still extremely difficult.

In our recently published paper in @narjournal.bsky.social we present a way forward.

🧵 ⤵️

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Zeynep Baharoglu @zeynepbaharoglu.bsky.social · Aug 23

Aggresomes protect mRNA under stress in Escherichia coli
www.nature.com/articles/s41... "prolonged stress leading to ATP depletion in Escherichia coli results in increased aggresome formation, compaction and selective mRNA enrichment within these aggresomes" 🦠 #microsky #rnasky

Aggresomes protect mRNA under stress in Escherichia coli - Nature Microbiology

Ribonucleoprotein aggresomes exclude ribonucleases and protect mRNA to promote rapid translation reactivation and cellular recovery after stress alleviation in Escherichia coli.

www.nature.com

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Nature Microbiology @natmicrobiol.nature.com · Aug 21

#News&Views

Bacterial prions form amyloids in response to phage infection and induce cell death to prevent viral replication, similar to the processes in fungi and across the tree of life.

#MicroSky

www.nature.com/articles/s41...

Amyloids in bacterial antiphage defence - Nature Microbiology

Bacterial prions form amyloids in response to phage infection and induce cell death to prevent viral replication, similar to the processes in fungi and across the tree of life.

www.nature.com

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Laura White @laurakwhite.bsky.social · Aug 22

Our paper is out in its final form! Back in April 2023, my coauthor Aleks Radakovic, then a PhD student, approached me after a talk in Chicago to ask what I thought an aminoacylated tRNA would look like if we pulled it through a @nanopore.
doi.org/10.1038/s414...

Nanopore sequencing of intact aminoacylated tRNAs - Nature Communications

Accurate protein synthesis depends on aminoacylated tRNAs, but their identities have been hard to measure. Here, authors present aa-tRNA-seq, a nanopore-based method that reveals the amino acid, seque...

doi.org

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ᐯIᑕTOᖇ ᑎIᘔET, ᗰᗪ @nizet.bsky.social · Aug 14

one small consolation of being a 60+ year old investigator is the ability to submit to 𝘔𝘈𝘛𝘜𝘙𝘌 family journals — sure they’re low impact (for our knees) but they have an early bird special if you submit between 4:00 and 5:30 on weekdays

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MACSYS @macsys.bsky.social · Aug 12

Meet the minds in MACSYS shaping the future with our series of profiles featuring our Postdoctoral Research Fellows. Dr TrungTin Nguyen is with MACSYS at QUT. His research investigates the mathematical & statistical foundations of #AI: www.linkedin.com/pulse/dr-tru...
@trungtinnguyen.bsky.social

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Debnath Ghosal @debnathghosal.bsky.social · Aug 2

In this system, bacteria detect phage infection through a sensor protein (YjbH) and respond by severing the infected part of the cell via aberrant division. The rest of the cell survives and continues growing happily, essentially, bacterial autotomy!

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Michael Deghelt @michaeldeghelt.bsky.social · Jul 29

#microsky
We challenge the long-standing view that peptidoglycan alone protects cells from bursting.

Our study shows that the periplasm — enclosed by OM–PG connections — acts as a pressure buffer essential for osmoprotection in Gram-negative bacteria.

📄 www.nature.com/articles/s41...

Peptidoglycan–outer membrane attachment generates periplasmic pressure to prevent lysis in Gram-negative bacteria - Nature Microbiology

Outer membrane attachment to peptidoglycan enables periplasmic pressure to build up and counter cytoplasmic turgor pressure, preventing lysis during osmotic challenges in Escherichia coli.

www.nature.com

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Nature Cell Biology @natcellbio.nature.com · Jul 29

🍹Sharma, Jiao et al. report glycosylation of small RNAs within #exosomes and propose a glycosylation-dependent mechanism for #RNA targeting into exosomes, which may influence intercellular communication and RNA stability in the extracellular environment.
👉https://rdcu.be/eykKY
bit.ly/455vyuP

Extracellular exosomal RNAs are glyco-modified - Nature Cell Biology

Sharma, Jiao and colleagues report glycosylation of small RNAs within exosomes and propose a glycosylation-dependent mechanism for RNA targeting into exosomes, which may influence intercellular commun...

bit.ly

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Trevor Lithgow @trevor-lithgow.bsky.social · Jul 17

We have been working on ways to find #phage with long shelf-life and this one is a winner. 12 months freeze-thawing as much as you like, no loss of viability
#phageSky #microSky

rdcu.be/ewnzT

Highly stable bacteriophages PIN1 and PIN2 have hallmarks of flagellotropic phages but infect immotile bacteria

npj Viruses - Bacteriophages (phages) are viruses that kill bacteria, with potential as antibacterial agents in industrial settings, agriculture, and human health. Here, we identified two phages,...

rdcu.be

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Nucleic Acids Research @narjournal.bsky.social · Jul 14

📣NAR Breakthrough Article!📣

✂️ Removal of a single tRNA^Gly gene copy in Staphylococcus aureus via CRISPR weakens the cell wall, biofilm formation and increases antibiotic susceptibility💊

📖 doi.org/10.1093/nar/...

#Microbiology #CRISPR #tRNA #AntibioticResearch #BacterialGenetics

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Matt Doyle @doylemt1.bsky.social · Jul 6

Check out these amazing lipid-monolayer-trapping oligomeric OMPs published by @lalouxlab.bsky.social and Lovering Labs. The BAM complex in these bugs must be wild!

www.nature.com/articles/s41...

A porin-like protein used by bacterial predators defines a wider lipid-trapping superfamily - Nature Communications

This study reveals that an outer membrane protein from the predator Bdellovibrio bacteriovorus forms a pentameric assembly that traps a lipid monolayer within. This allows the discovery of two superfa...

www.nature.com

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Chelsea Brown @chelseabrown.bsky.social · Jul 1

What better way to use my first proper post than to share my first big piece of post-doctoral work with @cg-martini.bsky.social!
Here, we used integrative modelling to build and simulate a mitochondrial cristae.
Find the paper here (rdcu.be/eujAC) or see below for a quick overview 👇

A stylized image of the molecular model of a mitochondrial crista, with proteins and lipids shown

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Laura Mike @lauraamike.bsky.social · Jul 3

It's seem like arginine is emerging as a major host cue. So we wrote an IAI minireview on how arginine levels vary between host tissues and affect microbial behavior.

The review is online today!
journals.asm.org/doi/epdf/10....

@brookeeryan3.bsky.social

#microsky

Known effects of arginine on Gram-positive and Gram-negative behavior

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Laura Mike @lauraamike.bsky.social · Jul 1

Our paper reporting a highly conserved mechanism by which arginine positively regulates hypervirulent K. pneumoniae mucoid phenotype is now fully online.
rdcu.be/euiWG

Summary picture is below, but details in the 🧵

Overview of paper revealing role of arginine in regulating K. pneumoniae mucoidy.

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Herve Celia @hervecelia.bsky.social · Jul 1

After an unexpectedly long editorial process, our paper describing the structures of the E coli TolAQR and TonB-ExbBD complexes is finally out. The coordinates are to be released on July 9th on the PDB.

www.nature.com/articles/s41...

Cryo-EM structures of the E. coli Ton and Tol motor complexes - Nature Communications

The Ton and Tol systems are bacterial energy-transducing complexes that use the proton motive force at the inner membrane to exert force on outer membrane proteins. Here the authors present the high-r...

www.nature.com

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Bergeron lab @bergeronlab.bsky.social · Jul 1

The peer-reviewed version of our article on the flagellum filament assembly is out!
A masterpiece from duo extraordinaire Kailin and Rosa, and an incredibly productive collaboration with Marc Erhardt and Morgan Beeby.

The structure of the complete extracellular bacterial flagellum reveals the mechanism of flagellin incorporation - Nature Microbiology

Cryo-electron microscopy analysis of the extracellular flagellum structure, including the hook–filament junction and filament cap complex, reveals mechanisms of flagellin incorporation into the growin...

www.nature.com

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