Lakshita Sharma
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lakshitaaa.bsky.social
Lakshita Sharma
@lakshitaaa.bsky.social
190 followers 280 following 9 posts
Learning from the mistakes of others who took my advice 🤐 | Exploring Proteostasis with Mass Spectrometry 🧑‍💻🥼 | Science & curiosity in constant motion 🛣️| 📍 Germany
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Reposted by Lakshita Sharma
realbiomassspec.bsky.social
Mass spectrometry-based human spatial omics: fundamentals, innovations, and applications #JBiomedSci link.springer.com/article/10.1...
Mass spectrometry-based human spatial omics: fundamentals, innovations, and applications - Journal of Biomedical Science
Mass spectrometry-based spatial omics is a powerful approach for visualizing the spatial organization of proteins, metabolites, lipids, and other biomolecules in situ, combining the molecular depth of...
link.springer.com
February 6, 2026 at 2:24 PM
Reposted by Lakshita Sharma
lindorfflarsen.bsky.social
A near-complete map of human cytosolic degrons and their relevance for disease

We measured degron potency of >200,000 30-residue tiles from >5,000 human proteins, and trained a model to predict degrons from sequence

Led by @vvouts.bsky.social in @rhp-lab.bsky.social

doi.org/10.1126/scia...
February 7, 2026 at 7:42 AM
Reposted by Lakshita Sharma
thevilchezlab.bsky.social
Our new review on proteostasis of organelles in aging & disease is now online in The FEBS Journal!
We discuss recent insights on how #proteostasis safeguards organelle integrity and function across the cell in #aging and disease
doi.org/10.1111/febs...
FEBS Press
Cells rely on regulated proteostasis mechanisms to keep their internal compartments functioning properly. When these mechanisms fail, damaged proteins accumulate, disrupting organelles, such as the n....
doi.org
February 5, 2026 at 8:34 AM
Reposted by Lakshita Sharma
paulschanda.bsky.social
📢 New preprint alert!
How do proteins enter mitochondria? We uncovered a surprising mechanism at the mitochondrial entry gate—using #NMR, in vivo single-particle tracking, yeast experiments, and MD simulations to crack the code.
www.biorxiv.org/content/10.6...
#StructuralBiology #Mitochondria
🧵 1/8
A dynamic displacement mechanism drives protein import into mitochondria
Most mitochondrial proteins are produced in the cytosol and imported through the translocase of the outer mitochondrial membrane (TOM) to reach their final destination. Although this protein entry gat...
www.biorxiv.org
January 14, 2026 at 7:16 PM
Reposted by Lakshita Sharma
uni-wuerzburg.de
New Paper out in @cellpress.bsky.social: How cancer cells protect themselves from the immune system. Cancer gene #MYC camouflages tumours by suppressing alarm signals. ‪@mycgyvers.bsky.social @cancergrand.bsky.social ‬‬‬‬ 📷 Daniel Peter
➡️ www.uni-wuerzburg.de/en/news-and-...
The authors of the Cell publication (from left to right): Martin Eilers, Amel Aziba, Leonie Uhl and Sinah Löbbert.
January 23, 2026 at 7:40 AM
Reposted by Lakshita Sharma
picottilab.bsky.social
We’re delighted to report a global structural proteome analysis within living cells!

doi.org/10.1038/s443...

#proteomics #structuralproteomics #massspectrometry #structuralbiology #ethz @imsb-eth.bsky.social
January 13, 2026 at 5:29 PM
Reposted by Lakshita Sharma
jeremybaskin.bsky.social
Thrilled to share our latest study, led by @reikatei.bsky.social, in @natchembio.nature.com! We began by asking a simple question—how do cells know if they have too much of a lipid in a particular membrane, and how do they respond to rectify this imbalance?
www.nature.com/articles/s41...
More info 👇
Membrane editing with proximity labeling reveals regulators of lipid homeostasis - Nature Chemical Biology
Coupling an optogenetic lipid-modifying enzyme with proximity labeling reveals protein networks and mechanisms regulating lipid homeostasis in the membranes of target organelles.
www.nature.com
January 7, 2026 at 2:59 PM
Reposted by Lakshita Sharma
vadim-demichev.bsky.social
We are delighted to release 𝐃𝐈𝐀-𝐍𝐍 𝟐.𝟑.𝟏, with a groundbreaking 𝐈𝐧𝐟𝐢𝐧𝐃𝐈𝐀 module for fast searches against huge databases and support for 𝐃𝐃𝐀 data.

Release notes: github.com/vdemichev/Di...
December 5, 2025 at 11:51 AM
Reposted by Lakshita Sharma
c0nc0rdance.bsky.social
University administration:
"We need you to complete 26 hours of lab safety training 🧪 each year so that everyone is safe and responsible."

Also university administration:
Stolen from the Internet, a sign that says "If Elevator does not move do a small jump it should move after", and a dirty-white freight elevator wall and aged elevator controls with only floors 1 and 2." I have no idea where this really is, I just stole it off Wikimedia.
October 15, 2025 at 3:09 PM
Reposted by Lakshita Sharma
liamfaller.bsky.social
Very cool stuff on the co-translational folding of dimers by Sander Tans and his team @amolf-nl.bsky.social:

www.nature.com/articles/s41...
Co-translational ribosome pairing enables native assembly of misfolding-prone subunits - Nature Communications
Protein complexes are essential for cell function. Here, authors show that paired ribosomes can help each other’s nascent chains fold correctly, enabling proper dimer assembly and preventing misfoldin...
www.nature.com
August 28, 2025 at 12:07 PM
Reposted by Lakshita Sharma
alirezamashaghi.bsky.social
We recently carried out the first single-molecule analysis of protein folding & unfolding in a cytosolic environment using optical tweezers—moving beyond simplistic buffer conditions to study proteins where they actually function.
advanced.onlinelibrary.wiley.com/doi/10.1002/...
#ChemSky #Biophysics
Cytosolic Interactome Protects Against Protein Unfolding in a Single Molecule Experiment
Single molecule experiments aimed at probing protein folding are typically performed in buffer conditions and cannot therefore be representative of the multi-faceted and complex cooperative interacti...
advanced.onlinelibrary.wiley.com
September 11, 2025 at 5:51 AM
Reposted by Lakshita Sharma
chercheurjuteux.com
ATP plays a structural role in Hsp90 function

www.nature.com/articles/s41...
ATP plays a structural role in Hsp90 function - Nature Communications
The exact role of ATP in the function of Hsp90, an essential molecular chaperone, is unclear since ATP binding but not hydrolysis is essential for viability. Here, the authors propose that ATP plays a...
www.nature.com
August 12, 2025 at 9:02 PM
Reposted by Lakshita Sharma
walterwchen.bsky.social
Our work on new paradigm in peroxisome biogenesis and PEX39 (1st human peroxisomal biogenesis protein (peroxin/PEX) found in > 20 yr) is out in @natcellbio.nature.com! Equal collab w/Tony Rodrigues (Jorge Azevedo lab), @dwendscheck.bsky.social (Bettina Warscheid lab)

www.nature.com/articles/s41...
PEX39 facilitates the peroxisomal import of PTS2-containing proteins - Nature Cell Biology
Chen et al. show that PEX39 cooperates with PEX7 in the peroxisomal import of proteins containing a PTS2 site and uncover an (R/K)PWE motif in PEX39 and PEX13 that binds to PEX7 and facilitates the im...
www.nature.com
July 30, 2025 at 12:16 PM
Reposted by Lakshita Sharma
apatgiri.bsky.social
Silencing mitochondrial gene expression in living cells www.science.org/doi/10.1126/...
Silencing mitochondrial gene expression in living cells
Mitochondria fulfill central functions in metabolism and energy supply. They express their own genome, which encodes key subunits of the oxidative phosphorylation system. However, central mechanisms u...
www.science.org
May 24, 2025 at 10:24 AM
Reposted by Lakshita Sharma
jcellsci.bsky.social
Kailash Venkatraman, Nicolas-Frédéric Lipp & @ibudin.bsky.social examine the similarities between prokaryotic intracytoplasmic membranes & mitochondrial IMs, & discuss whether cristae evolution has driven specialisation of the #mito lipidome.
journals.biologists.com/jcs/article/...
#JCSMitoSI
Bacterial ICMs exhibit internal invaginations that resemble eukaryotic cristae. (A)TEM images showing tubular cristae-like ICM compartments in free-living Desulfobacterota, and similar cristae-like ICM tubules in Desulfovibrio carbinolicus strains. The eukaryotic model organism S. cerevisiae exhibits tubular cristae structures, whereas the commonly used HEK293 cell line exhibits predominantly lamellar cristae structures. Scale bars: 500 nm. (B) Eukaryotic mitochondria tend to exhibit lamellar, tubular or discoidal cristae morphology, whereas orders of the class alphaproteobacteria exhibit a range of ICM morphologies. Lamellar-like ICMs, which exhibit parallel cytoplasmic protrusions that do not contact the inner membrane, are commonly observed in nitrogen-fixing and several methanotrophic alphaproteobacteria. (C) This simplified and non-exhaustive cladogram indicates the two domains of life – bacteria and archaea – with proto-mitochondria emerging in early alphaproteobacteria. Eukaryogenesis is marked by the horizontal transfer (red dotted arrow) from Bacteria to Hordarchaeales in Asgard archaea. §, †, #, * and ‡ connect species listed in B to their respective clades in C.
May 12, 2025 at 9:05 AM
Reposted by Lakshita Sharma
pwydorski.bsky.social
"Scouring the human Hsp70 network uncovers diverse chaperone safeguards buffering TDP-43 toxicity"
www.biorxiv.org/content/10.1...
Scouring the human Hsp70 network uncovers diverse chaperone safeguards buffering TDP-43 toxicity
Cytoplasmic aggregation and concomitant dysfunction of the prion-like, RNA-binding protein TDP-43 underpin several fatal neurodegenerative diseases, including amyotrophic lateral sclerosis. To elucida...
www.biorxiv.org
May 11, 2025 at 1:47 PM
Reposted by Lakshita Sharma
hirakdas.bsky.social
Happy to share our recent publication where we present the most up to date membrane protein inventory of #Glycosomes and identified the long sought trypanosomal PEX15.

Thanks to everyone involved for this amazing collaborative effort to combine #proteomics with #functional #analysis.
April 28, 2025 at 2:21 PM
Reposted by Lakshita Sharma
frezzalab.bsky.social
Mitochondria-organelle crosstalk in establishing compartmentalized metabolic homeostasis: Molecular Cell www.cell.com/molecular-ce...
Mitochondria-organelle crosstalk in establishing compartmentalized metabolic homeostasis
Mitochondria are metabolic hubs that communicate with other organelles via metabolite-, lipid-, and signaling-molecule exchange facilitated by membrane contact sites (MCSs). MCSs, regulated by tetheri...
www.cell.com
April 18, 2025 at 6:53 AM
Reposted by Lakshita Sharma
dplamartin.bsky.social
Excited to share our latest paper in Science Advances! We finally dissected a new pathway for mitochondrial quality control upon mtDNA replication stress! Let’s start a short thread! 🧵

www.science.org/doi/10.1126/...
Retromer promotes the lysosomal turnover of mtDNA
Lysosomal uptake of mtDNA controls mitochondrial quality.
www.science.org
April 4, 2025 at 6:42 PM
Reposted by Lakshita Sharma
slavov-n.bsky.social
Timescales in Cell Biology
April 4, 2025 at 11:32 AM
Reposted by Lakshita Sharma
korolchuklab.bsky.social
www.cell.com/trends/cell-...
The fate of mitochondrial respiratory complexes in aging
While mitochondrial dysfunction is one of the canonical hallmarks of aging, it remains only vaguely defined. Its core feature embraces defects in energy-producing molecular machinery, the mitochondria...
www.cell.com
March 27, 2025 at 9:32 AM
lakshitaaa.bsky.social
"When life gives you a 3D printer, you print a duck… that actually floats! Lab innovation at its finest. #ScienceMeetsQuack

@warscheidlab.bsky.social
March 26, 2025 at 8:20 AM
Reposted by Lakshita Sharma
odedrechavi.bsky.social
My face on zoom when i pretend to listen but work on something else
March 11, 2025 at 7:55 AM
Reposted by Lakshita Sharma
natcellbio.nature.com
☕️Interested in how cells respond to #mitochondria #ProteinImport stress? Read our Review article by Nikolaus Pfanner, Fabian den Brave & Thomas Becker.
👉🏽https://rdcu.be/ecb3x
bit.ly/3DgVkm5
Mitochondrial protein import stress - Nature Cell Biology
Cells respond to mitochondrial protein import stress by regenerating clogged import sites and inducing stress responses. Mitochondria are thus tightly integrated into the cellular proteostasis and str...
bit.ly
March 4, 2025 at 10:27 PM
Reposted by Lakshita Sharma
clausenlab.bsky.social
DUBs - unsung yet exciting players in the UPS: kay hofmann et al reveal bacterial DUBs with irreversible clippase activity, reshaping our view on ubiquitin dynamics ... and providing a tool to distinguish Ub and ISG15 signals in immune signalling. chapeau! www.sciencedirect.com/science/arti...
A family of bacterial Josephin-like deubiquitinases with an irreversible cleavage mode
Many intracellular bacteria secrete deubiquitinase (DUB) effectors into eukaryotic host cells to keep the bacterial surface or the enclosing vesicle m…
www.sciencedirect.com
March 4, 2025 at 8:40 AM