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Reposted

Zeynep Baharoglu @zeynepbaharoglu.bsky.social · 21h

Happy to share our new review in Royal Society Open Biology 🎉
tRNA-modifying enzymes in bacterial stress adaptation:
How tRNA mods rewire translation under oxidative + antibiotic stress (MoTTs, moonlighting, therapy angles).
Read: doi.org/10.1098/rsob...
#RNAsky #microsky #AMR #RNAmodifications

tRNA-modifying enzymes in bacterial stress adaptation | Open Biology

Transfer RNAs (tRNAs) and their modifications are central to bacterial translation and physiology, yet their roles in stress adaptation remain underexplored. While extensively studied in eukaryotes, a...

doi.org

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Reposted

Nilesh Banavali @nilesh-banavali.bsky.social · 5d

Interesting high resolution ab initio reconstruction workflow from Oli Clarke’s group. Wonder if it will work for signal subtracted parts of larger complexes.
www.biorxiv.org/content/10.1...

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Despite recent advances in data acquisition and algorithmic development, applying single particle cryogenic electron microscopy (cryoEM) to small proteins (<50kDa) remains challenging, even when high ...

www.biorxiv.org

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Reposted

David Kuster @rnadavid.bsky.social · 4d

Translation landscape of stress granules | Science Advances

www.science.org/doi/10.1126/...

Translation landscape of stress granules

Cryo–electron tomography visualizes stress granules in situ, revealing their spatial interplay with translation machinery.

www.science.org

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Reposted

David Balchin @davidbalchin.bsky.social · 19d

Our article on human multidomain protein biogenesis is now published in @natsmb.nature.com
www.nature.com/articles/s41...

The human ribosome modulates multidomain protein biogenesis by delaying cotranslational domain docking - Nature Structural & Molecular Biology

By studying dynamic folding intermediates on the human ribosome, Pellowe et al. show that newly made domains help each other to fold but do not stably interact until synthesis is complete, avoiding in...

www.nature.com

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Reposted

Alexey Amunts @amunts.bsky.social · 8d

Today, @rcsbpdb.bsky.social introduces Q-score in EM validation reports. Q-score assesses model-map fit. A new percentile slider will compare an entry’s average Q-score to the full archive and a resolution-matched subset. This would help judge whether the fit is typical for the reported resolution.

Introducing the first 3DEM Model-Map percentile slider to the wwPDB validation report

Introducing the latest wwPDB Percentile slider based on the Q-score model-map validation metric

www.rcsb.org

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soneya-majumdar.bsky.social @soneya-majumdar.bsky.social · 10d

www.science.org/doi/10.1126/...

Pre- and postantibiotic epoch: The historical spread of antimicrobial resistance

Plasmids are now the primary vectors of antimicrobial resistance, but our understanding of how human industrialisation of antibiotics influenced their evolution is limited by a paucity of data predati...

www.science.org

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Reposted

joe-wade.bsky.social @joe-wade.bsky.social · 27d

Check out our new paper: a review of translational coupling, the phenomenon where translation of one prokaryotic gene can promote translation of the gene downstream. We cover the history, and delve into the mechanism, which is still not fully understood. journals.asm.org/doi/10.1128/...

Translational coupling of neighboring genes in prokaryotes | Journal of Bacteriology

Prokaryotic genes are arranged in operons, with functionally related genes often located adjacent to one another (1). There are several ways in which the operonic organization of genes facilitates the...

journals.asm.org

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Reposted

Jon Paczkowski🌹 @paczkowskilab.bsky.social · Sep 8

It is rare as an editor to get to take on an article so uniquely in your wheelhouse. Sharing some wonderful work by Guarnaccia et al. from the Perez, Ng, and Neiditch labs. #structuralbiology of #pathogens @plos.org
dx.plos.org/10.1371/jour...

Structure-function studies of Vibrio cholerae quorum-sensing receptor CqsR signal recognition

Author summary Quorum sensing, a form of bacterial cell-cell communication, regulates diverse developmental behaviors. This study investigates CqsR, a transmembrane receptor in Vibrio cholerae, the ga...

dx.plos.org

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Reposted

Alice Ting @aliceyting.bsky.social · Aug 27

LOV-BirA, light regulated biotin ligase, engineered by Song-Yi Lee

www.sciencedirect.com/science/arti...

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Reposted

Alexey Amunts @amunts.bsky.social · Aug 22

Emmanuel Levy @elevylab.bsky.social has joined BlueSky 🌟 with a fantastic Cell paper with Shu-ou Shan, showing the interactome of the TOM complex and how cotranslational mito import prioritizes large globular domains. Beautiful science!
Give him a warm welcome 🎉
link: www.cell.com/cell/fulltex...

Principles of cotranslational mitochondrial protein import

Selective ribosome profiling reveals that nearly 20% of mitochondrial proteins in human cells are imported during translation on cytosolic ribosomes. Cotranslational import requires an N-terminal pres...

www.cell.com

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soneya-majumdar.bsky.social @soneya-majumdar.bsky.social · Aug 22

www.nature.com/articles/s41...

Site-selective protein editing by backbone extension acyl rearrangements - Nature Chemical Biology

A post-translational backbone extension acyl rearrangement (BEAR) reaction has now been developed that converts a ribosomal protein product into a new product containing a β-peptide, γ-peptide or δ-pe...

www.nature.com

soneya-majumdar.bsky.social @soneya-majumdar.bsky.social · Aug 22

www.nature.com/articles/s41...

Structures and mechanisms of U6 snRNA m6A modification by METTL16 - Nature Communications

U6 snRNA m6A modification by METTL16 is essential for accurate pre-mRNA splicing. Here, the authors determined the cryo-EM structure of METTL16 bound to U6 snRNA and SAM, revealing how its two domains...

www.nature.com

Reposted

Structural Biology @actacrystd.iucr.org · Aug 18

How can you find consistent biological 3D structures in heavily contaminated electron microscopy data sets without using any prior knowledge? #CryoEM #SingleParticle #Reconstruction doi.org/10.1107/S205...

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Reposted

Chi-Min Ho @cmholab.bsky.social · Aug 18

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH

A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug

Nature Structural & Molecular Biology - Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation...

rdcu.be

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soneya-majumdar.bsky.social @soneya-majumdar.bsky.social · Aug 18

www.nature.com/articles/s41...

Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug - Nature Structural & Molecular Biology

Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation elongation cycle and its perturbation by cabamiquine, a ...

www.nature.com

Reposted

Broad Institute @broadinstitute.org · May 28

A new pipeline could help scientists discover unique combinations of molecules that increase the effectiveness of antibiotics against drug-resistant bacteria. broad.io/CITADel-news @mit.edu @tuftsmedschool.bsky.social

Making antibiotics more potent against drug-resistant bacteria

Scientists find combinations of known antibiotics and other molecules that, together, have greater bacteria-killing power.

broad.io

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Reposted

Christine Dunham @dunhamlab.bsky.social · Aug 12

These exciting new results therefore have important implications for recoding of the genetic code for synthetic biology purposes and in the use of suppressor tRNAs for premature stop codon readthrough in human diseases. Congrats everyone!
rdcu.be/eAcLz

An RNA modification prevents extended codon-anticodon interactions from facilitating +1 frameshifting

Nature Communications - tRNAs contain many chemical modifications that regulate their function. Integrating smFRET and cryo-EM approaches, this work demonstrates that m1G37 in tRNAProL stabilizes...

rdcu.be

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Reposted

Christine Dunham @dunhamlab.bsky.social · Aug 12

Our new research was just published in Nature Communications! Graduate student Evy Kimbrough, Ha An Nguyen and Jacob Mattingly contributed to this work, Our results directly answer a long-standing and fundamental question in biology of how tRNA modifications influence mRNA reading frame maintenance.

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soneya-majumdar.bsky.social @soneya-majumdar.bsky.social · Aug 13

www.nature.com/articles/s41...

Deep learning reveals antibiotics in the archaeal proteome - Nature Microbiology

Use of artificial intelligence to mine proteomes of archaea led to the discovery of archaeasins, antimicrobials that kill drug-resistant bacteria in laboratory and animal models, offering a promising ...

www.nature.com

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Reposted

Joachim Frank @joachim123.bsky.social · Aug 6

nam02.safelinks.protection.outlook.com?url=https%3A...

Structures of Naked Mole-Rat, Tuco-Tuco, and Guinea Pig Ribosomes: Is rRNA Fragmentation Linked to Translational Fidelity?

Ribosomes are central to protein synthesis in all organisms. Among mammals, the ribosome functional core is highly conserved. Remarkably, two rodent species, the naked mole-rat (NMR) and tuco-tuco dis...

nam02.safelinks.protection.outlook.com

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Reposted

Martin Walsh @walshdls.bsky.social · Aug 4

krios 1 @diamondlightsource.bsky.social celebrated 10 years of user operations last week - many people thought we were mad to plonk it down in this spot in the synchrotron …but its still there as we ended up with a lot more in a very small space of time !

Titan krios I (a 300 KeV Cryo transmission electron microscope) with its 10 yr birthday hat in the experimental hall of Diamond Light Source.

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soneya-majumdar.bsky.social @soneya-majumdar.bsky.social · Aug 2

Congratulations, to you and your team!

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Reposted

Ahmad Jomaa @jomaalab.bsky.social · Aug 1

Check out our new preprint on the discovery of a molecular switch in NAC that mediates nascent chain sorting on the ribosome and prevents mitochondrial protein mistargeting by SRP. A great collaboration with the Shan Lab @Caltech and the Qi Lab @UVA: www.biorxiv.org/content/10.1...

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Reposted

cryoEM papers @cryoempapers.bsky.social · Jul 27

Human ribosome interactions reframe neomycin toxicity www.biorxiv.org/content/10.1101/2025.07.22.666027v1 #cryoem

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Reposted

George O'Toole (he/him/his) @geiselbiofilm.bsky.social · Jul 21

Editor's Pick: Shen, Ye et al. review recent literature on the role of bacterial ribosome heterogeneity on facilitating rapid response to stress.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology

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