Samrat Basak
@samratb.bsky.social
Single Molecule Localization and Super resolution microscopy enthusiast @labtinnefeld.bsky.social , LMU Munich
Reposted by Samrat Basak
Congrats to Juliana! She measures in ACS Nano the rate constants of nanosensors on the single molecule level. She found two kinetic pathways and one can use this knowledge for Kinetically Improved Sensor Selectivity (KISS). @solvationsci.bsky.social @ruhr-uni-bochum.de
tinyurl.com/738ypxf9
tinyurl.com/738ypxf9
Kinetic Insights Into Carbon Nanotube-Based Neurotransmitter Sensors by Single Molecule Experiments
Single-walled carbon nanotubes (SWCNTs) are powerful building blocks for near-infrared (NIR) fluorescent biosensors. They can be chemically tailored to detect specific biomolecules. Their performance ...
tinyurl.com
December 26, 2025 at 11:29 AM
Congrats to Juliana! She measures in ACS Nano the rate constants of nanosensors on the single molecule level. She found two kinetic pathways and one can use this knowledge for Kinetically Improved Sensor Selectivity (KISS). @solvationsci.bsky.social @ruhr-uni-bochum.de
tinyurl.com/738ypxf9
tinyurl.com/738ypxf9
Reposted by Samrat Basak
Excited that our work on Single-objective lattice light sheet microscopy with microfluidics for single-molecule super-resolution imaging of mammalian cells is now published in ACS Photonics!
doi.org/10.1021/acsp...
@pubs.acs.org #soTILT3D #soLLS #microscopy #nanoscale #imaging
doi.org/10.1021/acsp...
@pubs.acs.org #soTILT3D #soLLS #microscopy #nanoscale #imaging
Single-Objective Lattice Light Sheet Microscopy with Microfluidics for Single-Molecule Super-Resolution Imaging of Mammalian Cells
Single-molecule localization microscopy (SMLM) has redefined optical imaging by enabling imaging beyond the diffraction limit, allowing for nanoscale investigation into cellular architecture and molec...
doi.org
December 26, 2025 at 6:37 PM
Excited that our work on Single-objective lattice light sheet microscopy with microfluidics for single-molecule super-resolution imaging of mammalian cells is now published in ACS Photonics!
doi.org/10.1021/acsp...
@pubs.acs.org #soTILT3D #soLLS #microscopy #nanoscale #imaging
doi.org/10.1021/acsp...
@pubs.acs.org #soTILT3D #soLLS #microscopy #nanoscale #imaging
Reposted by Samrat Basak
✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
December 22, 2025 at 10:45 AM
✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
Reposted by Samrat Basak
Self-blinking dye restores efficient use of nanobodies in single-molecule localization microscopy. Link ot he @biorxivpreprint.bsky.social is here: www.biorxiv.org/content/10.6...
Self-blinking dye restores efficient use of nanobodies in single-molecule localization microscopy.
Direct STORM relies on robust fluorophore blinking; observations suggest that AlexaFluor647 underperforms when coupled to nanobodies, thereby undermining the advantages of nanobodies. We show that the...
www.biorxiv.org
December 22, 2025 at 10:45 AM
Self-blinking dye restores efficient use of nanobodies in single-molecule localization microscopy. Link ot he @biorxivpreprint.bsky.social is here: www.biorxiv.org/content/10.6...
Reposted by Samrat Basak
A cool thing was the FRET-based diffusion analysis that allowed a separate analysis of MET receptor monomers and dimers👇🏻
December 20, 2025 at 11:01 AM
A cool thing was the FRET-based diffusion analysis that allowed a separate analysis of MET receptor monomers and dimers👇🏻
Reposted by Samrat Basak
Excited to share our latest work! We introduce FRET-SOFI, a new method for super-resolved imaging of molecular interactions. By combining SOFI with FRET, we achieve subdiffraction resolution in live cells using standard wide-field microscopy.
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Super-resolved Imaging of Molecular Interactions Using FRET-SOFI
Super-resolution fluorescence microscopy is a powerful method to image molecular processes that occur beyond the diffraction limit of light. Förster resonance energy transfer (FRET) is widely used to ...
pubs.acs.org
December 10, 2025 at 9:00 AM
Excited to share our latest work! We introduce FRET-SOFI, a new method for super-resolved imaging of molecular interactions. By combining SOFI with FRET, we achieve subdiffraction resolution in live cells using standard wide-field microscopy.
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Reposted by Samrat Basak
This #Halloween 🦙🧛♂️ Alpacula brewed up #SMLM treat!
No more horror stories of no blinking & black holes ⚫️🔬Our single-domain anti-BOOdies 👻 #FluoTags and #SmartSecondaries now glow with self-blinking JF635b✨, delivering frightfully precise and simple #dSTORM imaging. 👉 bit.ly/4ffIkdB
No more horror stories of no blinking & black holes ⚫️🔬Our single-domain anti-BOOdies 👻 #FluoTags and #SmartSecondaries now glow with self-blinking JF635b✨, delivering frightfully precise and simple #dSTORM imaging. 👉 bit.ly/4ffIkdB
October 30, 2025 at 1:06 PM
This #Halloween 🦙🧛♂️ Alpacula brewed up #SMLM treat!
No more horror stories of no blinking & black holes ⚫️🔬Our single-domain anti-BOOdies 👻 #FluoTags and #SmartSecondaries now glow with self-blinking JF635b✨, delivering frightfully precise and simple #dSTORM imaging. 👉 bit.ly/4ffIkdB
No more horror stories of no blinking & black holes ⚫️🔬Our single-domain anti-BOOdies 👻 #FluoTags and #SmartSecondaries now glow with self-blinking JF635b✨, delivering frightfully precise and simple #dSTORM imaging. 👉 bit.ly/4ffIkdB
Reposted by Samrat Basak
We present a versatile microfluidics platform that makes multiplexed super-resolution imaging more accessible, reproducible, and high-throughput.
👉 BioRxiv: www.biorxiv.org/content/10.1...
#SuperResolution #DNApaint #Microfluidics #SMLM #OpenScience #multiplexing
👉 BioRxiv: www.biorxiv.org/content/10.1...
#SuperResolution #DNApaint #Microfluidics #SMLM #OpenScience #multiplexing
October 15, 2025 at 12:29 PM
We present a versatile microfluidics platform that makes multiplexed super-resolution imaging more accessible, reproducible, and high-throughput.
👉 BioRxiv: www.biorxiv.org/content/10.1...
#SuperResolution #DNApaint #Microfluidics #SMLM #OpenScience #multiplexing
👉 BioRxiv: www.biorxiv.org/content/10.1...
#SuperResolution #DNApaint #Microfluidics #SMLM #OpenScience #multiplexing
Reposted by Samrat Basak
NEW PREPRINT: Spatial mapping of cell-surface protein glycosylation at molecular resolution 🍭🍬🔬
For the first time, we can analyze the spatial architecture of the cell-surface glycoproteome at single-protein and single-sugar resolution. #glycotime
www.biorxiv.org/content/10.1...
For the first time, we can analyze the spatial architecture of the cell-surface glycoproteome at single-protein and single-sugar resolution. #glycotime
www.biorxiv.org/content/10.1...
Spatial mapping of cell-surface protein glycosylation at molecular resolution
Cell-surface protein glycosylation is a fundamental post-translational modification that plays a crucial role in membrane protein function and cellular behavior. However, elucidating the molecular spa...
www.biorxiv.org
October 2, 2025 at 11:05 AM
NEW PREPRINT: Spatial mapping of cell-surface protein glycosylation at molecular resolution 🍭🍬🔬
For the first time, we can analyze the spatial architecture of the cell-surface glycoproteome at single-protein and single-sugar resolution. #glycotime
www.biorxiv.org/content/10.1...
For the first time, we can analyze the spatial architecture of the cell-surface glycoproteome at single-protein and single-sugar resolution. #glycotime
www.biorxiv.org/content/10.1...
Reposted by Samrat Basak
Fiery kickoff for the 30th Single Molecule Workshop!
I’m super excited to see so many great scientists and big names in the field gathered here in Berlin.
Let’s go #WS30 🚀
#WS30 #SingleMolecule #Spectroscopy #Microscopy
I’m super excited to see so many great scientists and big names in the field gathered here in Berlin.
Let’s go #WS30 🚀
#WS30 #SingleMolecule #Spectroscopy #Microscopy
September 23, 2025 at 7:29 AM
Fiery kickoff for the 30th Single Molecule Workshop!
I’m super excited to see so many great scientists and big names in the field gathered here in Berlin.
Let’s go #WS30 🚀
#WS30 #SingleMolecule #Spectroscopy #Microscopy
I’m super excited to see so many great scientists and big names in the field gathered here in Berlin.
Let’s go #WS30 🚀
#WS30 #SingleMolecule #Spectroscopy #Microscopy
Reposted by Samrat Basak
Soo on stage presenting self-labeling enzymes with weak-affinity fluorophore labels for (1) high-density live SMLM using DeepSTORM (doi.org/10.1016/j.bp...) and (2) molecular counting ... great collaboration with @kjohnsson.bsky.social @juliankompa.bsky.social 🙏 #PQ30 @picoquant.bsky.social
September 23, 2025 at 8:46 AM
Soo on stage presenting self-labeling enzymes with weak-affinity fluorophore labels for (1) high-density live SMLM using DeepSTORM (doi.org/10.1016/j.bp...) and (2) molecular counting ... great collaboration with @kjohnsson.bsky.social @juliankompa.bsky.social 🙏 #PQ30 @picoquant.bsky.social
Reposted by Samrat Basak
Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11
Read the thread below for a summary of our findings 🧵1/11
September 17, 2025 at 2:55 PM
Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11
Read the thread below for a summary of our findings 🧵1/11
Reposted by Samrat Basak
Previous preprint is now published in RSC Chemical Biology.
If you are interested in plasma membrane labeling, see the paper! 👇
pubs.rsc.org/en/content/a...
If you are interested in plasma membrane labeling, see the paper! 👇
pubs.rsc.org/en/content/a...
September 8, 2025 at 8:02 AM
Previous preprint is now published in RSC Chemical Biology.
If you are interested in plasma membrane labeling, see the paper! 👇
pubs.rsc.org/en/content/a...
If you are interested in plasma membrane labeling, see the paper! 👇
pubs.rsc.org/en/content/a...
Reposted by Samrat Basak
Happy to contribute to this new work from @micoxscopy.bsky.social et al, helping apply SSQUASH to analyse the shape of spectrin rings along axons. Data analysis is the next frontier in SMLM, and I'm always up for chatting, sharing data and see how we can push things further!
August 13, 2025 at 10:38 AM
Happy to contribute to this new work from @micoxscopy.bsky.social et al, helping apply SSQUASH to analyse the shape of spectrin rings along axons. Data analysis is the next frontier in SMLM, and I'm always up for chatting, sharing data and see how we can push things further!
Reposted by Samrat Basak
🚨
New Preprint!
Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons.
▶️ www.biorxiv.org/content/10.1...
New Preprint!
Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons.
▶️ www.biorxiv.org/content/10.1...
August 19, 2025 at 3:32 PM
🚨
New Preprint!
Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons.
▶️ www.biorxiv.org/content/10.1...
New Preprint!
Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons.
▶️ www.biorxiv.org/content/10.1...
Reposted by Samrat Basak
🚨 New preprint on bioRxiv! 🚨
Smart microscopy🔬 is reshaping life sciences—but progress is slowed by fragmentation.
@lhinderling.bsky.social & the Smart Microsopy Working Group maps the field & proposes a path toward an open, interoperable future.
🧵👇
doi.org/10.1101/2025...
Smart microscopy🔬 is reshaping life sciences—but progress is slowed by fragmentation.
@lhinderling.bsky.social & the Smart Microsopy Working Group maps the field & proposes a path toward an open, interoperable future.
🧵👇
doi.org/10.1101/2025...
August 20, 2025 at 1:10 PM
🚨 New preprint on bioRxiv! 🚨
Smart microscopy🔬 is reshaping life sciences—but progress is slowed by fragmentation.
@lhinderling.bsky.social & the Smart Microsopy Working Group maps the field & proposes a path toward an open, interoperable future.
🧵👇
doi.org/10.1101/2025...
Smart microscopy🔬 is reshaping life sciences—but progress is slowed by fragmentation.
@lhinderling.bsky.social & the Smart Microsopy Working Group maps the field & proposes a path toward an open, interoperable future.
🧵👇
doi.org/10.1101/2025...
Proud to see this preprint out!
First time demonstrated Confocal MIET DNA-PAINT and has been a challenging but rewarding journey, and I’m excited about the possibilities it opens for 3D multiplexed nanoscopy in cells.
First time demonstrated Confocal MIET DNA-PAINT and has been a challenging but rewarding journey, and I’m excited about the possibilities it opens for 3D multiplexed nanoscopy in cells.
🚨 New preprint update! 🚨
We introduce Confocal MIET-PAINT — extending our 3D nanoscopy method with:
✨ High multiplexing capacity
✨ Isotropic nanometer-scale resolution (lateral + axial)
✨ Compatibility with custom & commercial confocal SMLM equipped with FLIM
www.biorxiv.org/content/10.1...
We introduce Confocal MIET-PAINT — extending our 3D nanoscopy method with:
✨ High multiplexing capacity
✨ Isotropic nanometer-scale resolution (lateral + axial)
✨ Compatibility with custom & commercial confocal SMLM equipped with FLIM
www.biorxiv.org/content/10.1...
August 23, 2025 at 10:27 AM
Proud to see this preprint out!
First time demonstrated Confocal MIET DNA-PAINT and has been a challenging but rewarding journey, and I’m excited about the possibilities it opens for 3D multiplexed nanoscopy in cells.
First time demonstrated Confocal MIET DNA-PAINT and has been a challenging but rewarding journey, and I’m excited about the possibilities it opens for 3D multiplexed nanoscopy in cells.
Excited to share — our project from @labtinnefeld.bsky.social with Heinrich Grabmayr from @jungmannlab.bsky.social has been selected for the CeNS Investment Fund from @cens-lmu.bsky.social !
www.cens.lmu.de/en/research/...
www.cens.lmu.de/en/research/...
August 12, 2025 at 5:45 PM
Excited to share — our project from @labtinnefeld.bsky.social with Heinrich Grabmayr from @jungmannlab.bsky.social has been selected for the CeNS Investment Fund from @cens-lmu.bsky.social !
www.cens.lmu.de/en/research/...
www.cens.lmu.de/en/research/...
Reposted by Samrat Basak
💥 We have 3 positions open in my lab in Basel, Switzerland🇨🇭:
#nanopores #smFRET #biomolecular #dynamics #singleMolecules
We’re looking for talented & ambitious new colleagues enthusiastic about biomolecular dynamics & single-molecule tech.
Please share broadly, thank you!🤝
schmid.chemie.unibas.ch
#nanopores #smFRET #biomolecular #dynamics #singleMolecules
We’re looking for talented & ambitious new colleagues enthusiastic about biomolecular dynamics & single-molecule tech.
Please share broadly, thank you!🤝
schmid.chemie.unibas.ch
Home | Research Group Schmid | University of Basel
schmid.chemie.unibas.ch
July 31, 2025 at 2:34 PM
💥 We have 3 positions open in my lab in Basel, Switzerland🇨🇭:
#nanopores #smFRET #biomolecular #dynamics #singleMolecules
We’re looking for talented & ambitious new colleagues enthusiastic about biomolecular dynamics & single-molecule tech.
Please share broadly, thank you!🤝
schmid.chemie.unibas.ch
#nanopores #smFRET #biomolecular #dynamics #singleMolecules
We’re looking for talented & ambitious new colleagues enthusiastic about biomolecular dynamics & single-molecule tech.
Please share broadly, thank you!🤝
schmid.chemie.unibas.ch
Great lab and great PI to start with ...!
🚨 2 × PhD positions @EPFL! 🚨
Help us push the boundaries of fluorescence microscopy - DNA nanotech, custom optics & spatial omics in Lausanne 🇨🇭. Start Jan 2026. Send CV + motivation + 2 refs → [email protected]
#PhD #Hiring #microscopy #SuperResolution #SpatialOmics #DNAPAINT #FLASHPAINT
Help us push the boundaries of fluorescence microscopy - DNA nanotech, custom optics & spatial omics in Lausanne 🇨🇭. Start Jan 2026. Send CV + motivation + 2 refs → [email protected]
#PhD #Hiring #microscopy #SuperResolution #SpatialOmics #DNAPAINT #FLASHPAINT
July 31, 2025 at 2:51 PM
Great lab and great PI to start with ...!
Reposted by Samrat Basak
🔬🧬 New in @natcomms.nature.com: Nanoscale 3D DNA tracing in non‑denatured cells maps the #3Dgenome at nm‑resolution and reveals Cohesin‑dependent dynamic loops! 🚀 #OpenAccess
www.nature.com/articles/s41...
@ellenberglab.bsky.social @jungmannlab.bsky.social
#Genomics #SuperResolution
www.nature.com/articles/s41...
@ellenberglab.bsky.social @jungmannlab.bsky.social
#Genomics #SuperResolution
Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ - Nature Communications
A nanoscale 3D DNA tracing workflow visualizes Cohesin-dependent loops in single, structurally well-preserved cells. Computer simulations based on the tracing data give further insight into how Cohesi...
www.nature.com
July 23, 2025 at 12:39 PM
🔬🧬 New in @natcomms.nature.com: Nanoscale 3D DNA tracing in non‑denatured cells maps the #3Dgenome at nm‑resolution and reveals Cohesin‑dependent dynamic loops! 🚀 #OpenAccess
www.nature.com/articles/s41...
@ellenberglab.bsky.social @jungmannlab.bsky.social
#Genomics #SuperResolution
www.nature.com/articles/s41...
@ellenberglab.bsky.social @jungmannlab.bsky.social
#Genomics #SuperResolution
Reposted by Samrat Basak
A "Small" update on studying the coating of single DNA nanostructures with a fluorescence lifetime sensor! 🧬🔬 Was a fun project at the interface of DNA nanotech and single molecule photophysics working in a great team! 🤓 🙌 @ameliehj.bsky.social
onlinelibrary.wiley.com/doi/10.1002/...
onlinelibrary.wiley.com/doi/10.1002/...
July 8, 2025 at 6:07 PM
A "Small" update on studying the coating of single DNA nanostructures with a fluorescence lifetime sensor! 🧬🔬 Was a fun project at the interface of DNA nanotech and single molecule photophysics working in a great team! 🤓 🙌 @ameliehj.bsky.social
onlinelibrary.wiley.com/doi/10.1002/...
onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Samrat Basak
It's here! ✨
The #ExSRRF protocol paper is out 😍. Full details for merging Expansion Microscopy with our eSRRF analysis. From cells to tissue. A partnership with @puellesv.bsky.social's lab. Lead by Dominik Kylies and @hannah-superres.bsky.social + colleagues. #ExM #eSRRF
➡️ Open link: rdcu.be/euvEw
The #ExSRRF protocol paper is out 😍. Full details for merging Expansion Microscopy with our eSRRF analysis. From cells to tissue. A partnership with @puellesv.bsky.social's lab. Lead by Dominik Kylies and @hannah-superres.bsky.social + colleagues. #ExM #eSRRF
➡️ Open link: rdcu.be/euvEw
July 3, 2025 at 8:44 AM
It's here! ✨
The #ExSRRF protocol paper is out 😍. Full details for merging Expansion Microscopy with our eSRRF analysis. From cells to tissue. A partnership with @puellesv.bsky.social's lab. Lead by Dominik Kylies and @hannah-superres.bsky.social + colleagues. #ExM #eSRRF
➡️ Open link: rdcu.be/euvEw
The #ExSRRF protocol paper is out 😍. Full details for merging Expansion Microscopy with our eSRRF analysis. From cells to tissue. A partnership with @puellesv.bsky.social's lab. Lead by Dominik Kylies and @hannah-superres.bsky.social + colleagues. #ExM #eSRRF
➡️ Open link: rdcu.be/euvEw
Reposted by Samrat Basak
🎉 Major achievement for #LMU in the Excellence Strategy: All seven proposed Clusters of Excellence have successfully passed the review process and will be funded for seven years as of 2026. An outstanding result! 🎉 #ExcellenceCluster #LMUMunich #research Read more here: www.lmu.de/en/newsroom/...
May 22, 2025 at 4:25 PM
🎉 Major achievement for #LMU in the Excellence Strategy: All seven proposed Clusters of Excellence have successfully passed the review process and will be funded for seven years as of 2026. An outstanding result! 🎉 #ExcellenceCluster #LMUMunich #research Read more here: www.lmu.de/en/newsroom/...
Reposted by Samrat Basak
The #ClustersOfExcellence have been selected: today, the Excellence Commission approved 70 projects for funding. 45 clusters will continue and 25 will be newly established. Funding starts on 1 Jan 2026 for 7 years, with €539 million per year. The full list: www.dfg.de/resource/blo... 1/3
May 22, 2025 at 6:10 PM
The #ClustersOfExcellence have been selected: today, the Excellence Commission approved 70 projects for funding. 45 clusters will continue and 25 will be newly established. Funding starts on 1 Jan 2026 for 7 years, with €539 million per year. The full list: www.dfg.de/resource/blo... 1/3