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Reposted by Katie Downes

MFS 2025 Conference @msf25.bsky.social · 12d

🔬At #MFS25, Giulia Zanetti from @TheCrick unveiled the molecular architecture of the early secretory pathway using cryo-ET & super-resolution microscopy resolving the debate on COPII vesicles and mapping ER-to-Golgi transport in human cells.

🔗https://mfs2025.com/

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Reposted by Katie Downes

Ben Engel @cellarchlab.com · Nov 23

We're looking for an awesome computational #postdoc to join our group @PioneerCampus. You'll explore native cells with #cryoET, get long-term funding (5+ years), and live in Munich! For more info, see our new Cell Architecture Lab website:...

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Katie Downes @dr-downes.bsky.social · Aug 20

I attended this course last year and cannot recommend it enough!

Rory Hennell James @rorydhj.bsky.social · Aug 18

This was a brilliant course last year; I highly recommend it to anyone looking to improve their CryoFIB and CryoET skills!

Max Planck Institute of Biophysics @mpibp.bsky.social · Aug 18

We’re hosting the @embo.org Practical Course on In-situ structural biology by CryoFIB and CryoET in April 2026! 🧬❄️ You can expect hands-on training, cutting-edge methods, and a chance to work alongside leading experts in structural biology. 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...

Katie Downes @dr-downes.bsky.social · Jul 30

Very excited to announce that my first, first author paper, is now on BioRxiv! In this paper we unambiguously show #COPII coated vesicles in unperturbed human cells for the very first time!!

Make my day and check it out!
www.biorxiv.org/content/10.1...

#Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬

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Katie Downes @dr-downes.bsky.social · Jun 16

This revolutionised our segmentation of coated membranes.

Membrain V1 didn't recognise coated membranes - lots of manual editing was needed. Membrain V2 - beautiful segmentation, chefs kiss!

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Katie Downes @dr-downes.bsky.social · Apr 28

Yes! we reproducibly get ~150nm thin lamella with very few failures and regularly run sessions for ~48hrs.

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Katie Downes @dr-downes.bsky.social · Apr 28

We have the Aquilos2 which is fab!

Our only complaint is the iFLM filter cubes are optimised for room temperature but the spectra of a number of fluorophores changes at cryo and so their peak excitation and emission wavelengths are filtered out leading to poor signal at cryo vs RT

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Lorenz Lamm @lorenzlamm.bsky.social · Apr 25

🦠🧠 MemBrain update! 🧠🦠
We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics.
🔗 Preprint: doi.org/10.1101/2024...
🔗 Code: github.com/CellArchLab/...
🧵(1/6) #TeamTomo

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Katie Downes @dr-downes.bsky.social · Mar 28

Ready for BBCs #AQuestionofScience here @crick.ac.uk 🔬🧬🥼🧪🦠

Reposted by Katie Downes

Misha Kudryashev @mishakudryashev.bsky.social · Mar 28

We are looking for a PhD student/Postdoc to develop methods of cryo-ET and/or study the structure and function of membrane proteins. Great team at the MDC in Berlin.

It's a broad call, please apply and share!
jobrxiv.org/job/max-delb...

PhD /Postdoc in In Situ Structural Biology by Cryo-ET

Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

jobrxiv.org

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Katie Downes @dr-downes.bsky.social · Mar 25

I am thrilled to announce my and @giuliazanetti.bsky.social's latest review "Mechanisms of COPII coat assembly and cargo recognition in the secretory pathway" in @natrevmcb.bsky.social with @springernature.com.

Check it out here!
rdcu.be/eeY1J

#COPII #CryoEM #TeamTomo #MembraneTrafficking #ERES

Mechanisms of COPII coat assembly and cargo recognition in the secretory pathway

Nature Reviews Molecular Cell Biology - Coat protein complex II (COPII) subunits assemble to form membrane transport carriers at ER exit sites, thereby concentrating secretory cargoes and...

rdcu.be

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Katie Downes @dr-downes.bsky.social · Mar 7

Does anyone have any idea what this wavey guy is? 🌊🌊🌊🌊#TeamTomo #CryoET

In-situ tomogram of milled RPE-1 cells.

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Katie Downes @dr-downes.bsky.social · Mar 5

@giuliazanetti.bsky.social something worth trying with our data maybe?

Reposted by Katie Downes

Ishier Raote @ishier.bsky.social · Jan 15

Exciting news! #MembraneTrafficking online seminars with @felixmendu.bsky.social @franbottanelli.bsky.social and me are back!
Check the spectacular Jan-April 2025 lineup.
Details (Zoom links, calendar invites) on the mailing list, Slack group & website: felixcampelo.wixsite.com/membranetraf...

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Katie Downes @dr-downes.bsky.social · Dec 19

Both very well deserved!

Katie Downes @dr-downes.bsky.social · Nov 28

Lets play...

What
Am
I
Looking
At??

Low mag tomogram of milled RPE-1s.
Any guesses of whats going on here? #TeamTomo

Prize? idk... bragging rights? The feeling of superiority?

I'll collect a high mag tomo tomorrow, so the answer may actually be revealed! (mysteries mysteries mysteries).

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Katie Downes @dr-downes.bsky.social · Nov 27

We won’t be there I’m afraid, but we are always happy to chat!

Reposted by Katie Downes

Ferdos Abid Ali @abidalilab.bsky.social · Nov 27

If you’re interested in how molecular motors coordinate to power intracellular trafficking events (e.g. endocytosis), I’ve got a PhD position available to study this at vibrant Bristol uni. In this project, we will capture highly dynamic events by #cryoEM, single molecule imaging & cell biology. 🔬❄️🧪

This scheme shows the cargo adaptor FHF in the middle with arrows pointing to distinct microtubule and actin-based motors (dynein-dynactin, KIFC1, KIF1C and myosins V, IX and X as well as Tropomyosin 1). It also shows a link to Rab5 marked early endosomes. The hypothesis is that FHF coordinates multi motor binding at the early stages of endocytosis. The PhD project would address the hierarchy of motor binding and how these motors coordinate to allow the newly formed vesicle to transition from the actin to MT cytoskeletal network.

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Reposted by Katie Downes

MRC-LMB Cell Biology Division @cellbiol-mrclmb.bsky.social · Nov 12

This year’s UK Membrane Trafficking Meeting is on Dec 16th at King’s College London (Guy’s Campus, London Bridge).
Registration closes 9th December. Sign up is here.
estore.kcl.ac.uk/conferences-...
Thanks go to @J_Cell_Sci for sponsoring student/postdoc prizes!

UK Membrane Trafficking Meeting 2024 | King's College London eStore

Registration for the 2024 UK Membrane Trafficking Meeting, held at KCL.  Registration per person, to include sandwich lunch and coffee breaks and even

estore.kcl.ac.uk

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Reposted by Katie Downes

Ishier Raote @ishier.bsky.social · Nov 11

📢 Announcing the Membrane Trafficker Starter Pack! This list highlights researchers advancing our understanding of membrane trafficking pathways. This is just a beginning—help expand the list!
go.bsky.app/J9GvL3S