Lightnews — Scholar-powered news

Reposted by Kay Schink

Reto Fiolka @retof.bsky.social · 2d

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues.
www.biorxiv.org/content/10.1...

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Erdinc Sezgin @sciezgin.bsky.social · May 13

New paper out! Would you like to fluorescently label the plasma membrane in live, fixed or permeabilized cells? I tested different lipid structures and labelling approaches to decide what makes a good membrane labelling probe for such imaging experiments!
www.biorxiv.org/content/10.1...

Plasma membrane labelling efficiency, internalization and partitioning of functionalized fluorescent lipids as a function of lipid structure

Labeling the plasma membrane for advanced imaging remains a significant challenge. For time-lapse live cell imaging, probe internalization and photobleaching are major limitations affecting most membr...

www.biorxiv.org

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Reposted by Kay Schink

André Nadler @nadlerlab.bsky.social · Aug 21

Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism.
www.nature.com/articles/s41...

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Kay Schink @kschink.bsky.social · Aug 21

Amazing story, André. Congratulations!

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Clausen Lab @clausenlab.bsky.social · Aug 21

big one! a decade in the making. nadler & team develop tools to track lipid transport in living cells at single-species resolution. turns out non-vesicular, asymmetry-driven transport is the main architect of organelle identity. feels like the dawn of a golden era for lipid biology. rdcu.be/eBGJv

Quantitative imaging of lipid transport in mammalian cells

Nature - Directional, non-vesicular lipid transport is responsible for fast, species-selective lipid sorting into organelle membranes.

rdcu.be

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tafesselab.bsky.social @tafesselab.bsky.social · Aug 12

Have you published lipid-protein interactome data? Please let us know, we’d love to include it in the repository. The goal is to build a centralized hub for the scientific community.

Huge thanks to Gaelen Guzman, a graduate student/postdoc in the lab who built it from scratch.

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Ragnhild Eskeland @ragnhildes.bsky.social · Aug 12

We are hiring! A 3-year postdoc in #Chromatin #Cancer #Epigenetics at University of Oslo, Norway and co-supervised by @amathelier.bsky.social You will be part of @cancelluio.bosky.social. We focus on #Sarcoma #Epigenome #CRISPR and Drug screen studies. Find out more and apply here: shorturl.at/DtLHM

Postdoctoral Research Fellowship in Chromatin Biology and Cancer Epigenetics (283381) | University of Oslo

Job title: Postdoctoral Research Fellowship in Chromatin Biology and Cancer Epigenetics (283381), Employer: University of Oslo, Deadline: Wednesday, August 20, 2025

shorturl.at

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Reposted by Kay Schink

Anthony Mathelier @amathelier.bsky.social · Jul 22

🚨 We're hiring! NCMBM @ncmbm.bsky.social is looking for 2 new group leaders to join our vibrant research community. Ready to build your own group with strong support and a solid startup package?

Interested and attending #ISMBECCB2025? Let’s connect!

🔗 www.jobbnorge.no/en/available...

Group Leader (282908) | University of Oslo

Job title: Group Leader (282908), Employer: University of Oslo, Deadline: Monday, September 8, 2025

www.jobbnorge.no

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Alfred Millett-Sikking @amsikking.bsky.social · Jul 7

The power of basic optical design knowledge and CAD!

-> Here's a fast laser point scanner that connects to a commercial microscope. The design, build and alignment are relatively simple. Much harder is agile software control that integrates with the existing system... Custom hardware is hard!

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Felix Campelo @felixmendu.bsky.social · Jul 4

Announcing a new @embo.org Workshop on "Intracellular mechanics and organelle mechanobiology" that we are organizing with Michael Krieg and Verena Ruprecht at @icfo.eu (Barcelona) on 16-20 February, 2026. Please, repost and spread the word! 🙏 #EMBOmechanobio

meetings.embo.org/event/26-org...

Intracellular mechanics and organelle mechanobiology

Mechanobiology is an interdisciplinary field that emerges at the cross-section of biology, physics and engineering. It aims to understand how living cells, tissues and animals sense and respond to me…

meetings.embo.org

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Reposted by Kay Schink

Kai Johnsson @kjohnsson.bsky.social · Jul 3

Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology

SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

www.nature.com

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Joachim Goedhart @joachimgoedhart.bsky.social · Jun 26

De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...

$Fig. 4 Super-resolution microscopy in live and fixed cells with Rhobin. (A) Rhobin transiently binds rhodamine molecules and thereby constantly replenishes signal lost through photobleaching of dyes. (B-D) No-wash STED microscopy of live U2OS cells transiently expressing LifeAct-Rhobin2 labeled with 100 nM JFX650. (C, D) Zoom-ins of regions highlighted in (B) imaged either with confocal or STED microscopy. Scale bar: 20 μm (B) and 10 μm (C, D). (E-H) Rhobin enables timelapse STED microscopy of fast ER dynamics with minimal signal loss. U2OS stably expressing N-terminal tag fusions of SEC61B were stained with 1 μM Halo-JFX650 (HaloTag7, reHaloF) or 2 μM SiRhP (Rhobin2) and imaged at a frame rate of 0.387 fps for at least 100 time points. (F, G) Selected frames from timelapse acquisitions of Rhobin2:SEC61B labeled with SiRhP (F) or HaloTag7:SEC61B labeled with Halo-JFX650 (G). Scale bar: 2 μm. (H) Signal .CC-BY-NC 4.0 International licenseavailable under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprintthis version posted June 25, 2025.;https://doi.org/10.1101/2025.06.24.661379doi:bioRxiv preprint 22 loss during timelapse imaging. Mean ± SD signal inside cells over time for indicated constructs. (I-K) PAINT-type super-resolution microscopy with Rhobin. Rhobin2-SEC61B was transiently expressed in U2OS cells and labeled with 5 nM SiRhP after chemical fixation. Imaging under no- wash conditions and near-TIRF illumination. (I) Repeated, but transient binding of SiRhP molecules to Rhobin2 at low nanomolar concentrations can be observed as intensity bursts in intensity time traces extracted from a diffraction-limited area. See movie S3 for raw data of binding-induced blinking. (J) Sum image across 10,000 frames of an image stack, simulating a diffraction-limited image. (K). Reconstructed image from localized molecules in the full stack.$

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Michael Way @drmichaelway.bsky.social · Feb 20

🔎 Looking to start a PhD in Sept 2025?
The Crick Institute spring round for PhD recruitment is open!
If you are interested in actin cytoskeleton and imaging 🔬 then check out my project on role of Arp2/3 iso-complexes in regulating invadopodia👇please RT

www.crick.ac.uk/careers-stud...

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Kay Schink @kschink.bsky.social · Feb 20

nature.com/articles/s41467-025-57053-9
Finally this work is out - we show that short sequence motifs direct proteins to specific peroxisomal sub-domains. Fantastic collaboration with @bangebalcony.bsky.social , Johannes Freitag @unimarburg.bsky.social and many more! @uio.no @cancelluio.bsky.social

Peroxisomal core structures segregate diverse metabolic pathways - Nature Communications

Peroxisomes contain detergent resistant core structures. Bäcker et al. show that these core structures contain diverse enzymes and serve to enable metabolic compartmentalization of the peroxisome lume...

nature.com

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Kay Schink @kschink.bsky.social · Feb 20

Very proud to be part of that team - this was a really long term project with lots of amazing people!

Gert Bange @bangebalcony.bsky.social · Feb 20

www.nature.com/articles/s41... Great Joint Venture with Johannes Freitag @synmikro.bsky.social @unimarburg.bsky.social

Peroxisomal core structures segregate diverse metabolic pathways - Nature Communications

Peroxisomes contain detergent resistant core structures. Bäcker et al. show that these core structures contain diverse enzymes and serve to enable metabolic compartmentalization of the peroxisome lume...

www.nature.com

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Gert Bange @bangebalcony.bsky.social · Feb 20

www.nature.com/articles/s41... Great Joint Venture with Johannes Freitag @synmikro.bsky.social @unimarburg.bsky.social

Peroxisomal core structures segregate diverse metabolic pathways - Nature Communications

Peroxisomes contain detergent resistant core structures. Bäcker et al. show that these core structures contain diverse enzymes and serve to enable metabolic compartmentalization of the peroxisome lume...

www.nature.com

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Reposted by Kay Schink

Fran Bottanelli @franbottanelli.bsky.social · Feb 19

Long in the making but our protocol for gene editing is online! FAB CRISPR is super easy and allows the generation of N and C terminal fusions with TurboID, SNAP, Halo and mStayGold. A single cloninf step to generate HDR templates! arxiv.org/abs/2502.12675 1/n

Fast Antibiotic resistance-Based gene editing of mammalian cells with CRISPR-Cas9 (FAB-CRISPR)

Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency causing low knock-in rates. We ...

arxiv.org

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The Berro lab @berrolab.bsky.social · Jan 20

Molecular force measurements in vivo are notoriously difficult. We present a new class of programmable force sensors based on engineered coiled-coils, with modular readouts, including fluorescence and luminescence. doi.org/10.1101/2025...

Genetically encoded mechano-sensors with versatile readouts and compact size

Mechanical forces are critical for virtually all fundamental biological processes, yet quantification of mechanical forces at the molecular scale in vivo remains challenging. Here, we present a new st...

doi.org

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Institut Pasteur | 130 years of biomedical research @pasteur.fr · Jan 14

Welcome to the official Bluesky page of the Institut Pasteur!

🔬 Explore with us the frontiers of biomedical research

🧪 Follow us to uncover groundbreaking discoveries

💡 Share knowledge for global health

🌍 Join a community passionate about progress and open science.

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Jean-Yves Tinevez @jytinevez.bsky.social · Jan 16

Pasteur is on bluesky!

Institut Pasteur | 130 years of biomedical research @pasteur.fr · Jan 16

The Institut Pasteur has decided to leave X (formerly Twitter) to join BlueSky! 🦋
The Institut Pasteur made this decision due to several serious issues observed on the X platform since its acquisition. Join us here to continue advocating for science. www.pasteur.fr/en/home/pres...

Institut Pasteur decides to leave X

The Institut Pasteur, a research organization which for more than 130 years has been committed to tackling infectious diseases, sharing knowledge and defending science, has decided to leave X because ...

www.pasteur.fr

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Joachim Goedhart @joachimgoedhart.bsky.social · Jan 14

Genetically Encoded Fluorogenic DNA Aptamers for Imaging Metabolite in Living Cells
pubs.acs.org/doi/10.1021/...

Genetically Encoded Fluorogenic DNA Aptamers for Imaging Metabolite in Living Cells

Genetically encoded fluorescent protein and fluorogenic RNA sensors are indispensable tools for imaging biomolecules in cells. To expand the toolboxes and improve the generalizability and stability of...

pubs.acs.org

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Robert Arkowitz @robertarkowitz.bsky.social · Jan 7

Very nice

Claire Deo @clairedeo.bsky.social · Jan 7

We made a photoswitchable HaloTag (psHaloTag), which can reversibly turn-on fluorogenic dyes upon illumination 💡. Congrats to Franzi, Bego and all co-authors, check out our preprint below 👇
www.biorxiv.org/content/10.1...

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Christophe 🔬 L @christlet.bsky.social · Jan 6

How it started How it's going

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Loren Looger lab @loogerl.bsky.social · Dec 19

have ppl found any funders that support toolmaking? I want to keep making tools for people, but there is a seemingly irresistible force pushing us towards hypothesis-driven research. In my experience, funders don't want to hear about tech dev. Any insights? for both neuro & climate would be great tx

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Christian Mosimann @chrmosimann.bsky.social · Dec 18

"Till you have sequence confirmation, you are not cloning but clowning." 🧬⭕🤡
- Lab Saying.

#PILife #AlwaysBeCloning

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