Coronaviruses, like many other positive-sense single-stranded RNA viruses, rely on intracellular membrane remodeling to create a protected environment for efficient replication to occur. The resulting double membrane vesicles (DMVs) have characteristic pores formed from the non-structural viral proteins nsp3 and nsp4, while enzymes responsible for RNA replication, including the polymerase nsp12, are contained inside. Recent structural work has elucidated the nature of the pores, but how the polymerase and other viral proteins are recruited to the DMV represents a major gap in our current knowledge. Here we present a novel, step-by-step structural model of how the pores initially form from the uncleaved polyprotein, how protease activity is initiated, and how the viral replication machinery is trapped within the DMV.

Semliki Forest virus (SFV) belongs to the Alphavirus genus in the Togaviridae family and can cause a spectrum of clinical manifestations in the host, including fever, rash, arthritis, and even symptoms of encephalitis. Here, we reveal that ARRDC1 is a ...

第一次世界大戦中。世界は一瞬「異世界」という他ない世界と繋がった。　そこでもたらされたのが、道具に人格を与え、制御させる技術「プラズマインドスフィア」だった。　便利だが大規模な電力設備が必要となるそれを、人類は艦船に搭載。　海を往く人類の良き友人として、人類を補佐し続けた。　それは、軍事面でも変わらない。　これは、アメリカ海軍所属、ＣＶ－５　ＵＳＳヨークタウンと呼ばれた艦艇と、そこに宿ったプラズマ...

Lin et al. demonstrate that the NSP4 T492I substitution associates with the increased infectivity, transmissibility, and attenuated pathogenicity observed in recent SARS-CoV-2 variants, including Delt...

NSP4 protein in rotavirus is found crucial for infection severity by disrupting calcium signals. This breakthrough could pave the way for innovative treatments for rotavirus infections.

Researchers at Baylor College of Medicine and collaborating institutions have improved our understanding of how rotavirus, the most common cause of acute...

The NSP4 viroporin initiates an innate immune response and intercellular calcium waves that contribute to pathogenesis.

Researchers at Baylor College of Medicine and collaborating institutions have improved our understanding of how rotavirus, the most common cause of acute gastroenteritis in children, makes people sick.

The NSP3/4/12 axis in β-coronaviruses orchestrates the formation of double-membrane vesicles (DMVs) and the assembly of replication organelles by utilizing

そこは大通りから離れた路地にある喫茶店『Bonheur（ボヌール）』。喫茶店『Bonheur』を中心に始まる、ほのぼのな日常。アナタだけの『小さな幸せ』、ぜひ見つけてみませんか？

Upon entry into the host cell, the non-structural proteins 3, 4, and 6 (Nsp3, Nsp 4, and Nsp6) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate the formation of double-membra...