This Medical News article discusses new research identifying cellular changes in the brains of younger athletes with repeated head impacts, which may provide an early warning for development of chroni...

Alzheimer’s Disease (AD) is a neurodegenerative disorder that affects memory, behavior, and cognition and is the leading cause of dementia.1 The underlying

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In amyotrophic lateral sclerosis (ALS), nuclear depletion and cytoplasmic aggregation of the RNA-binding protein TDP-43 cause widespread dysregulation of mRNA splicing. Two recent studies have now rev...

Glycosphingosines (GlycoSphs), a class of simple glycosphingolipids (GSLs), are abundant in the brain and crucial in neurodegenerative diseases. Specific GlycoSphs, including glucosyl-sphingosine (GlcSph) and galactosyl-sphingosine (GalSph), exhibit distinct biological roles due to subtle structural isomeric differences, including anomeric configurations (α, β). These variations influence solubility, membrane interactions, and pathological outcomes, with GlcSph linked to immune modulation and GalSph to neurodegeneration. Standard mass spectrometry (MS) enables rapid analysis of biochemical mixtures but lacks the capability to separate isomers. The integration of ion mobility spectrometry (IMS) with MS overcomes this limitation by providing orthogonal analyte separation based on size and shape. In particular, a high-resolution variant of IMS called cyclic IMS (cIMS) has successfully separated some stereoisomeric mixtures; however, the structural diversity of GSLs remains challenging to fully characterize due to their high degree of structural similarity among multiple stereoisomers. This study investigates the potential of crown ether non-covalent modification to enable the separation of four GlycoSph isomers (GlcSph-α, GlcSph-β, GalSph-α, and GalSph-β). Whereas cIMS analysis of all six binary mixtures without crown ether modification resulted in no observable separation after >50 passes, cIMS analysis of complexes with 15-crown-5, 18-crown-6, and dibenzo-21-crown-7 resulted in varying degrees of separation for GlycoSph stereoisomer mixtures. In particular, 18-crown-6 complexation resulted in three identifiable peaks when separating the mixture of all four isomers and enabled separation of five out of six possible GlycoSph binary mixtures. This approach demonstrates promise for precise monitoring of GlycoSph isomer levels, necessary for biomarker identification and GlycoSph mechanism studies. Graphical abstract

In lieu of animal experiments, researchers from the University of Rochester are using state-of-the-art microchips with human tissue to better understand how the brain operates under healthy conditions...

Ocular fluids provide a window into the health of the eye and the development of various pathologies. However, their study usually involves invasive techniques.