Juan Ortiz Mateu
@ortizmateujuan.bsky.social
PhD student at the @memprotlabuv.bsky.social - University of Valencia
Especially interested in the biogenesis of viral proteins.
Especially interested in the biogenesis of viral proteins.
Pinned
The sequence and structural integrity of the SARS-CoV-2 Spike protein transmembrane domain is crucial for viral entry - Communications Biology
A study using SARS-CoV-2 pseudotyped viruses and testing Spike protein fusion capacity demonstrates that its transmembrane domain is essential for viral entry and suggests the presence of an intramemb...
www.nature.com
The first chapter of my PhD is finally out on @commsbio.nature.com
We show that the transmembrane domain of SARS-CoV-2 Spike protein is not just a passive anchor to the virion surface, but a key player in the viral entry process.
We show that the transmembrane domain of SARS-CoV-2 Spike protein is not just a passive anchor to the virion surface, but a key player in the viral entry process.
Reposted by Juan Ortiz Mateu
Our story on GroEL/ES action during cotranslational folding is now published @natcomms.nature.com. Led by former-student Alzbeta Roeselova, and in collaboration with Rado Enchev's lab @crick.ac.uk.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
GroEL/ES chaperonin unfolds then encapsulates a nascent protein on the ribosome - Nature Communications
The GroEL/ES chaperonin can act during protein synthesis to promote folding. Here, Roeselová et al. show how GroEL captures, remodels and sequesters nascent proteins in its central chamber, while they...
www.nature.com
November 14, 2025 at 1:49 PM
Our story on GroEL/ES action during cotranslational folding is now published @natcomms.nature.com. Led by former-student Alzbeta Roeselova, and in collaboration with Rado Enchev's lab @crick.ac.uk.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Juan Ortiz Mateu
How is N-glycosylation regulated? Check out our story, part of a terrific collaboration with our colleagues at Stanford and Northwestern. Congratulations to everyone!
rdcu.be/eQNsJ
rdcu.be/eQNsJ
Structural basis of regulated N-glycosylation at the secretory translocon
Nature - The structural mechanism of a natively isolated GRP94 folding intermediate tethered to a CCDC134-bound translocon is described, revealing how the nascent chain remodels the translocon to...
rdcu.be
November 19, 2025 at 7:16 PM
How is N-glycosylation regulated? Check out our story, part of a terrific collaboration with our colleagues at Stanford and Northwestern. Congratulations to everyone!
rdcu.be/eQNsJ
rdcu.be/eQNsJ
Reposted by Juan Ortiz Mateu
The first chapter of my PhD is finally out on @commsbio.nature.com
We show that the transmembrane domain of SARS-CoV-2 Spike protein is not just a passive anchor to the virion surface, but a key player in the viral entry process.
We show that the transmembrane domain of SARS-CoV-2 Spike protein is not just a passive anchor to the virion surface, but a key player in the viral entry process.
The sequence and structural integrity of the SARS-CoV-2 Spike protein transmembrane domain is crucial for viral entry - Communications Biology
A study using SARS-CoV-2 pseudotyped viruses and testing Spike protein fusion capacity demonstrates that its transmembrane domain is essential for viral entry and suggests the presence of an intramemb...
www.nature.com
November 18, 2025 at 12:33 PM
The first chapter of my PhD is finally out on @commsbio.nature.com
We show that the transmembrane domain of SARS-CoV-2 Spike protein is not just a passive anchor to the virion surface, but a key player in the viral entry process.
We show that the transmembrane domain of SARS-CoV-2 Spike protein is not just a passive anchor to the virion surface, but a key player in the viral entry process.
Reposted by Juan Ortiz Mateu
Glushkova, Böhm, & Beck @maxplanck.de develop a publicly available computational method to measure the thickness of biological membranes in cryo-electron tomograms. Analysis of algae & human cells reveals systematic membrane thickness variations within & across organelles rupress.org/jcb/article/...
November 4, 2025 at 5:30 PM
Glushkova, Böhm, & Beck @maxplanck.de develop a publicly available computational method to measure the thickness of biological membranes in cryo-electron tomograms. Analysis of algae & human cells reveals systematic membrane thickness variations within & across organelles rupress.org/jcb/article/...
Reposted by Juan Ortiz Mateu
Lipid bilayer thinning near a ubiquitin ligase selects ER membrane proteins for degradation www.biorxiv.org/content/10.1...
Lipid bilayer thinning near a ubiquitin ligase selects ER membrane proteins for degradation
Misfolded or unassembled membrane proteins in the endoplasmic reticulum (ER) are polyubiquitinated, translocated into the cytosol, and degraded by the proteasome, a poorly understood process that is c...
www.biorxiv.org
November 3, 2025 at 3:35 PM
Lipid bilayer thinning near a ubiquitin ligase selects ER membrane proteins for degradation www.biorxiv.org/content/10.1...
Reposted by Juan Ortiz Mateu
Providing order amongst constant traffic: #LMBResearch from Sean Munro (@cellbiol-mrclmb.bsky.social) with John Briggs (@mpibiochem.bsky.social) reveals how GOLPH3 allows COPI vesicles to distinguish between Golgi residents & ER-bound proteins.
Read more: www2.mrc-lmb.cam.ac.uk/protein-sort...
Read more: www2.mrc-lmb.cam.ac.uk/protein-sort...
October 6, 2025 at 8:07 AM
Providing order amongst constant traffic: #LMBResearch from Sean Munro (@cellbiol-mrclmb.bsky.social) with John Briggs (@mpibiochem.bsky.social) reveals how GOLPH3 allows COPI vesicles to distinguish between Golgi residents & ER-bound proteins.
Read more: www2.mrc-lmb.cam.ac.uk/protein-sort...
Read more: www2.mrc-lmb.cam.ac.uk/protein-sort...
Reposted by Juan Ortiz Mateu
Elaborate machinery for inserting proteins into the outer membrane of bacteria www.nature.com/articles/d41...
Elaborate machinery for inserting proteins into the outer membrane of bacteria
Bacteria in the gut use an unusual molecular apparatus to insert complex proteins into their outer membrane.
www.nature.com
October 2, 2025 at 9:29 AM
Elaborate machinery for inserting proteins into the outer membrane of bacteria www.nature.com/articles/d41...
Reposted by Juan Ortiz Mateu
Our article on human multidomain protein biogenesis is now published in @natsmb.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
The human ribosome modulates multidomain protein biogenesis by delaying cotranslational domain docking - Nature Structural & Molecular Biology
By studying dynamic folding intermediates on the human ribosome, Pellowe et al. show that newly made domains help each other to fold but do not stably interact until synthesis is complete, avoiding in...
www.nature.com
September 19, 2025 at 1:52 PM
Our article on human multidomain protein biogenesis is now published in @natsmb.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Juan Ortiz Mateu
27 simulations of capturing hundreds of lipid scrambling events reveal mechanisms of lipid translocation.
However, according to reviewers, more evidence is needed on outside-the-groove scramblase activity.
buff.ly/LTxy4yX
However, according to reviewers, more evidence is needed on outside-the-groove scramblase activity.
buff.ly/LTxy4yX
September 10, 2025 at 10:02 AM
27 simulations of capturing hundreds of lipid scrambling events reveal mechanisms of lipid translocation.
However, according to reviewers, more evidence is needed on outside-the-groove scramblase activity.
buff.ly/LTxy4yX
However, according to reviewers, more evidence is needed on outside-the-groove scramblase activity.
buff.ly/LTxy4yX
Reposted by Juan Ortiz Mateu
Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
August 27, 2025 at 4:14 PM
Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Juan Ortiz Mateu
💫NEW: @annaleder.bsky.social et al. show that the chaperones PDIA6, Hsp70 BiP, ERdj3, PDIA1 and Hsp90 form co-condensates within the endoplasmic reticulum, enhancing folding and preventing misfolding of client proteins. @masgu.bsky.social @hillerlab.bsky.social
bit.ly/47b3Mjk
bit.ly/47b3Mjk
A multichaperone condensate enhances protein folding in the endoplasmic reticulum - Nature Cell Biology
Leder et al. show that the chaperones PDIA6, Hsp70 BiP, ERdj3, PDIA1 and Hsp90 form co-condensates within the endoplasmic reticulum, enhancing folding and preventing misfolding of client proteins.
bit.ly
August 22, 2025 at 5:36 PM
💫NEW: @annaleder.bsky.social et al. show that the chaperones PDIA6, Hsp70 BiP, ERdj3, PDIA1 and Hsp90 form co-condensates within the endoplasmic reticulum, enhancing folding and preventing misfolding of client proteins. @masgu.bsky.social @hillerlab.bsky.social
bit.ly/47b3Mjk
bit.ly/47b3Mjk
Reposted by Juan Ortiz Mateu
Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
August 21, 2025 at 5:19 AM
Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Juan Ortiz Mateu
Check out our new preprint on the discovery of a molecular switch in NAC that mediates nascent chain sorting on the ribosome and prevents mitochondrial protein mistargeting by SRP. A great collaboration with the Shan Lab @Caltech and the Qi Lab @UVA: www.biorxiv.org/content/10.1...
August 1, 2025 at 3:27 PM
Check out our new preprint on the discovery of a molecular switch in NAC that mediates nascent chain sorting on the ribosome and prevents mitochondrial protein mistargeting by SRP. A great collaboration with the Shan Lab @Caltech and the Qi Lab @UVA: www.biorxiv.org/content/10.1...
Reposted by Juan Ortiz Mateu
Very excited to announce that my first, first author paper, is now on BioRxiv! In this paper we unambiguously show #COPII coated vesicles in unperturbed human cells for the very first time!!
Make my day and check it out!
www.biorxiv.org/content/10.1...
#Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬
Make my day and check it out!
www.biorxiv.org/content/10.1...
#Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬
July 30, 2025 at 3:28 PM
Very excited to announce that my first, first author paper, is now on BioRxiv! In this paper we unambiguously show #COPII coated vesicles in unperturbed human cells for the very first time!!
Make my day and check it out!
www.biorxiv.org/content/10.1...
#Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬
Make my day and check it out!
www.biorxiv.org/content/10.1...
#Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬
Reposted by Juan Ortiz Mateu
ChimeraX daily builds can predict binding affinity of small molecules using Boltz 2 on your Mac, Windows or Linux computer. www.rbvi.ucsf.edu/chimerax/dat...
July 24, 2025 at 2:44 AM
ChimeraX daily builds can predict binding affinity of small molecules using Boltz 2 on your Mac, Windows or Linux computer. www.rbvi.ucsf.edu/chimerax/dat...
Reposted by Juan Ortiz Mateu
Proud to share our first lab pre-print: “SND3 is the membrane insertase within a fungal multipass translocon” where @tzujingyang.bsky.social solved the structure of a ribosome-associated SND3-translocon complex involved in ER membrane protein insertion ➡️ doi.org/10.1101/2025...
July 12, 2025 at 12:49 AM
Proud to share our first lab pre-print: “SND3 is the membrane insertase within a fungal multipass translocon” where @tzujingyang.bsky.social solved the structure of a ribosome-associated SND3-translocon complex involved in ER membrane protein insertion ➡️ doi.org/10.1101/2025...
Reposted by Juan Ortiz Mateu
Folddisco finds similar (dis)continuous 3D motifs in large protein structure databases. Its efficient index enables fast uncharacterized active site annotation, protein conformational state analysis and PPI interface comparison. 1/9🧶🧬
📄 www.biorxiv.org/content/10.1...
🌐 search.foldseek.com/folddisco
📄 www.biorxiv.org/content/10.1...
🌐 search.foldseek.com/folddisco
July 7, 2025 at 8:21 AM
Folddisco finds similar (dis)continuous 3D motifs in large protein structure databases. Its efficient index enables fast uncharacterized active site annotation, protein conformational state analysis and PPI interface comparison. 1/9🧶🧬
📄 www.biorxiv.org/content/10.1...
🌐 search.foldseek.com/folddisco
📄 www.biorxiv.org/content/10.1...
🌐 search.foldseek.com/folddisco
Reposted by Juan Ortiz Mateu
Engineered ATP synthase with multiple a-subunits!
Engineering of ATP synthase for enhancement of proton-to-ATP ratio
www.nature.com/articles/s41...
Engineering of ATP synthase for enhancement of proton-to-ATP ratio
www.nature.com/articles/s41...
Engineering of ATP synthase for enhancement of proton-to-ATP ratio - Nature Communications
FoF1-ATP synthase converts proton motive force into ATP, with the H+/ATP ratio typically ranging from 2.7 to 5. Here, the authors engineered this enzyme to form multiple peripheral stalks, achieving a...
www.nature.com
July 3, 2025 at 10:09 AM
Engineered ATP synthase with multiple a-subunits!
Engineering of ATP synthase for enhancement of proton-to-ATP ratio
www.nature.com/articles/s41...
Engineering of ATP synthase for enhancement of proton-to-ATP ratio
www.nature.com/articles/s41...
Reposted by Juan Ortiz Mateu
Joining the race to the bottom has never been cooler...
www.biorxiv.org/node/4580343...
www.biorxiv.org/content/10.1...
www.biorxiv.org/node/4580343...
www.biorxiv.org/content/10.1...
A cellular entity retaining only its replicative core: Hidden archaeal lineage with an ultra-reduced genome
Defining the minimal genetic requirements for cellular life remains a fundamental question in biology. Genomic exploration continually reveals novel microbial lineages, often exhibiting extreme genome...
www.biorxiv.org
May 25, 2025 at 2:48 PM
Joining the race to the bottom has never been cooler...
www.biorxiv.org/node/4580343...
www.biorxiv.org/content/10.1...
www.biorxiv.org/node/4580343...
www.biorxiv.org/content/10.1...
Reposted by Juan Ortiz Mateu
🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
June 19, 2025 at 8:58 AM
🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Reposted by Juan Ortiz Mateu
Amazingly, this gave us a high-confidence transmembrane channel for both mouse and human NS3, with oleic acids forming a bilayer-like pattern.
Our group now call this the “fatty acid hack.”
The #alphafold predictions with and without fatty acids shown below 👇
Our group now call this the “fatty acid hack.”
The #alphafold predictions with and without fatty acids shown below 👇
June 18, 2025 at 11:47 AM
Amazingly, this gave us a high-confidence transmembrane channel for both mouse and human NS3, with oleic acids forming a bilayer-like pattern.
Our group now call this the “fatty acid hack.”
The #alphafold predictions with and without fatty acids shown below 👇
Our group now call this the “fatty acid hack.”
The #alphafold predictions with and without fatty acids shown below 👇
Reposted by Juan Ortiz Mateu
#FluorescenceFriday (we are watching vimentin (HaloTag7) in U2OS cells co-stained with Nile Red) #livecell #longterm
December 13, 2024 at 4:23 PM
#FluorescenceFriday (we are watching vimentin (HaloTag7) in U2OS cells co-stained with Nile Red) #livecell #longterm
Reposted by Juan Ortiz Mateu
Excited to unveil Boltz-2, our new model capable not only of predicting structures but also binding affinities! Boltz-2 is the first AI model to approach the performance of FEP simulations while being more than 1000x faster! All open-sourced under MIT license! A thread… 🤗🚀
June 6, 2025 at 1:46 PM
Excited to unveil Boltz-2, our new model capable not only of predicting structures but also binding affinities! Boltz-2 is the first AI model to approach the performance of FEP simulations while being more than 1000x faster! All open-sourced under MIT license! A thread… 🤗🚀