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Reposted by Claire Dessalles

Gautam Dey @gautamdey.bsky.social · 4d

If you’re applying to your dream lab for an internship/PhD/postdoc, always send a second email 1-2 weeks after the first one if you don’t hear back.

I promise we will be grateful rather than annoyed. My email inbox is a disaster and I’m quite junior - and very few of us have secretarial support

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Reposted by Claire Dessalles

Miki Ebisuya @ebisuyamiki.bsky.social · 11h

New preprint from the lab! We discovered a transient fluidization in the basal region of human forebrains by tracking microdroplets in cerebral organoids.This “basal fluidization”, absent in gorilla and mouse, may contribute to greater surface expansion in human forebrains
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doi.org/10.1101/2025...

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Joachim Goedhart @joachimgoedhart.bsky.social · 12d

Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM by Juan Manuel Garcia-Arcos and team: www.biorxiv.org/content/10.1...

Frequency dependence of fast-lifetime FLIM and fits for C2C12 cells.

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Reposted by Claire Dessalles

Spirochrome @spirochrome.com · 1d

An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable.

Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!

Juanma García-Arcos @juanmagararc.bsky.social · 3d

🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.

www.biorxiv.org/content/10.1...

Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM

Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...

www.biorxiv.org

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Claire Dessalles @clairedessalles.bsky.social · 1d

All you've ever wanted to know about FLIM analysis (and its application in Flipper-TR)!😱🤩🔬

Juanma García-Arcos @juanmagararc.bsky.social · 3d

🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.

www.biorxiv.org/content/10.1...

Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM

Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...

www.biorxiv.org

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Reposted by Claire Dessalles

Development @dev-journal.bsky.social · 6d

Modifying mechanical constraint guides gastruloid patterning

This Research Highlight showcases the work from Judith Pineau @judithpineau.bsky.social, Jerome Wong-Ng @jeromewongng.bsky.social, Thomas Gregor @thomasgregor.bsky.social and colleagues:

journals.biologists.com/dev/article/...

Figure 2 - Gene expression patterning and transcriptional profiles in hydrogel-grown gastruloids. (A) Representative immunofluorescence images of gastruloids at 120 h. Gastruloids were grown in standard culture conditions (Ctrl, no hydrogel) or embedded in hydrogels with increasing concentrations (0.7 mM, 0.8 mM and 1.0 mM) at 96 h post-seeding. Nuclei (blue), BRA (purple) and SOX2 (yellow) are labeled. (B,C) Normalized expression profiles of SOX2 (B) and BRA (C) along the AP axis, demonstrating consistent expression patterns across conditions. (D) Principal component analysis of bulk RNA-seq experiments (three replicates), revealing clear separation between Ctrl and noCHI (red), a negative control for gastruloid formation, while hydrogel-embedded conditions cluster near Ctrl. (E) Number of significantly differentially expressed (DE) genes compared to Ctrl, showing substantial transcriptional changes in noCHI and minimal changes in hydrogel-embedded conditions (0.7 mM, 0.8 mM and 1.0 mM). Shown here are representative data and analysis for one experimental replicate. See Fig. S3 for two additional replicates.

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Reposted by Claire Dessalles

Judith Pineau @judithpineau.bsky.social · 5d

In this beautiful system, we could show uncoupling of gene expression profiles, morphology and patterning… #NotInTheGenes 😉 (also includes a method to do long term 🔬 of elongating gastruloids)
Thank you to all involved @jeromewongng.bsky.social @thomasgregor.bsky.social @alexandremayran.bsky.social

Development @dev-journal.bsky.social · 6d

Modifying mechanical constraint guides gastruloid patterning

This Research Highlight showcases the work from Judith Pineau @judithpineau.bsky.social, Jerome Wong-Ng @jeromewongng.bsky.social, Thomas Gregor @thomasgregor.bsky.social and colleagues:

journals.biologists.com/dev/article/...

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Reposted by Claire Dessalles

Christophe 🔬 L @christlet.bsky.social · 12d

#FluorescenceFriday: getting ready for #GEF25, playing with Ciarán's stunning ExM data

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Dr Elodie Chabrol @eloscicomm.bsky.social · 15d

👩‍🔬J'ai commencé un kit de démarrage "chercheuses en France" go.bsky.app/TBEzKC #FemmesEnScience

J'ai besoin de votre aide pour le remplir et donner de la visibilité aux chercheuses que vous connaissez et qui sont sur Bluesky !
▶️Taguez celles que vous connaissez, je les ajoute !

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Claire Dessalles @clairedessalles.bsky.social · 23d

Super clear thread for super cool science!

Shweta Nandakumar @bucklingmt.bsky.social · 24d

Despite their high rigidity, microtubules (MTs) dynamically bend and buckle in cells. MT-self repair is inherent to their metastable nature, but it remains unclear whether just inherent repair mechanisms are sufficient to maintain MT integrity🧵 (1/9)
www.biorxiv.org/content/10.1...

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Claire Dessalles @clairedessalles.bsky.social · 27d

I love the pearling instability at the end ! 😍 What happens in the happy attached flagellum to stabilize its tubular shape?

Thibaut Brunet @thibautbrunet.bsky.social · 29d

Most striking, in some instances of spontaneous flagellar detachment, the flagellum can glide on its own - mercilessly leaving the cell body behind, stabbing it on the way if need be (don't do this at home).

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Thibaut Brunet @thibautbrunet.bsky.social · 29d

Most striking, in some instances of spontaneous flagellar detachment, the flagellum can glide on its own - mercilessly leaving the cell body behind, stabbing it on the way if need be (don't do this at home).

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Reposted by Claire Dessalles

Hao Yin @haoyin.bsky.social · 27d

#SmoothMuscleCell #Piezo1 Small intestinal motility

SMC Piezo1 KO in 4~6 wk-old🐭
👉Muscularis thinning
👉⏬Food consumption
👉⏬SMC density w/o⏫apoptosis
👉⏬small intestinal motility
👉⏬Muscularis Orai1/3 Trpc4 IP3R ⏫Cav1.2 Ano1
👉⏬carbachol-induced tension

#CommsBio 2025
www.nature.com/articles/s42...

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Reposted by Claire Dessalles

Biophysical Journal @biophysj.bsky.social · 28d

In the New and Notable "Pulling Frank-Starling into another dimension,"
Megan L. McCain highlights the paper "Biaxial length-tension relationship in single cardiac myocytes ." buff.ly/XliGXNu

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Reposted by Claire Dessalles

Carlo Barnaba 🇮🇹🇲🇽 @barnabalab.bsky.social · 27d

CellPose 4.0 is a beast. Segmented human microglia, in brightfield. Credit to Sydney Houser.

Human microglia bright field image segmented with CellPose 4.

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Claire Dessalles @clairedessalles.bsky.social · Sep 5

Congrats David, well done! 👏

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Reposted by Claire Dessalles

David Brückner @davidbrueckner.bsky.social · Sep 4

Super excited that our group will be supported by an ERC Starting Grant!

In project "InfoFate" we will study how cells use information in dynamical, neighborhood & mechanical signals to make decisions.

We'll have PhD and Postdoc positions available, please get in touch if interested!

Biozentrum, University of Basel @biozentrum.unibas.ch · Sep 4

Why do some cells become neurons and others muscle?
Physicist Prof. David Brückner @davidbrueckner.bsky.social @biozentrum.unibas.ch @unibas.ch receives a prestigious #ERCStartingGrant to uncover how cells make life-shaping decisions. @erc.europa.eu
www.biozentrum.unibas.ch/news/detail/...

ERC Starting Grant for Physicist David Brückner

How do cells decide which role to take on in the body? Prof. David Brückner from the Biozentrum of the University of Basel is investigating this and similar questions. Over the next five years, a Star...

www.biozentrum.unibas.ch

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Xavier Trepat @xaviertrepat.bsky.social · Sep 1

New preprint! 🚨 We uncover a slow adaptation to stretch that links star-bundling of keratin filaments with nuclear escape from its keratin cage. Led by @tomgolde.bsky.social‬ 🙌
@IBECBarcelona
www.biorxiv.org/content/10.1...

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Julia Eckert @juliaeckert.bsky.social · Aug 15

Now published in @natcomms.nature.com! 🥳

👉 rdcu.be/eATn3

We developed image analysis tools to capture the nematic orientation field of 3D tissue surfaces. Tested on epithelial aggregates, zebrafish hearts, myoblasts on spheres & micro-vessels, we combined soft matter physics with exp. biology.

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Baptiste Lacoste @blacoste.bsky.social · Jul 25

Very cool new paper by @thegulab.bsky.social published in Cell, showing that arterial endothelial coupling via gap junctions determines the speed and spread of vasodilation induced by neuronal activity ! #cerebrovascular #bloodflow #endothelium

Brain endothelial gap junction coupling enables rapid vasodilation propagation during neurovascular coupling

Vasodilatory signals are rapidly communicated across long distances by endothelial-endothelial gap junctions, enabling coordinated dilation of the arterial network during neurovascular coupling.

www.cell.com

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Claire Dessalles @clairedessalles.bsky.social · Jul 17

Our study on endothelial mechanics made the news! 🤩

Super nice and clear article on our findings and their implications.
www.snexplores.org/article/how-...

Here’s why your blood vessels don’t burst under pressure

Cells lining the blood vessels reorganize their inner structures to handle stressful boosts in pressure.

www.snexplores.org

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Roux Lab, Geneva @rouxlab.bsky.social · Jul 10

Nice discussions after the talk given online yesterday at cytophys.org, presenting the work of @pauguillamat.bsky.social @clairedessalles.bsky.social @yamini-ravichan.bsky.social @oriolmb.bsky.social and Tithi Mandal. Available on the lab youtube channel at:
youtu.be/g-7HomnPdtk

Cell forces, order and flows during In vitro reconstitution of morphogenesis

YouTube video by Roux Lab - University of Geneva

youtu.be

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Markku Hakala @mhakala.bsky.social · Jun 22

I'm grateful for the Research Council of Finland for granting me the Academy Research fellowship! After getting over the shock I will soon move back to Finland to start as a group leader at the Institute of Biotechnology @helsinki.fi. Looking for a PhD student soon! www.aka.fi/en/about-us/...

RCF winter call results: funding decisions in biosciences, health and environmental research

The Research Council of Finland’s Scientific Council for Biosciences, Health and the Environment has granted funding for 38 Academy Research Fellowships, 12 Clinical Researchers and 76 Academy P...

www.aka.fi

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Claire Dessalles @clairedessalles.bsky.social · Jun 3

Thank you @cp-newton.bsky.social for the very nice spotlight on our latest paper on endothelial mechanics!
www.cell.com/newton/fullt...

Blood vessels under stress

Writing in Nature Physics, Claire Dessalles, Avin Babataheri, Guillaume Salbreux, and colleagues show the effect of luminal pressure on cytoskeletal dynamics and tissue mechanics in an experimental mo...

www.cell.com

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Reposted by Claire Dessalles

Newton @cp-newton.bsky.social · Dec 16

Hi everyone! We are Newton, the flagship physics journal for Cell Press that launches in 2025. Starting our first post with an

🎇 EXCITING ANNOUNCEMENT 🎇

Our first article and Perspective are now online!!! Check them out @ www.cell.com/newton/newar... !

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