Yojet
@yojet.bsky.social
Final year PhD candidate working to uncover the role of lipid 5-phosphatase in human brain development @NCBS, India.
Interested in neuroimmune interactions, physiology, and microglia.
Interested in neuroimmune interactions, physiology, and microglia.
Pinned
Yojet
@yojet.bsky.social
· 18d
Very excited to share that my PhD work is out!
Neurons carry ~5,000–10,000 molecules of PI(4,5)P₂ per μm² of membrane. What happens to human brain development when that number changes? I address this using a disease-in-a-dish model of Lowe Syndrome:
www.embopress.org/doi/10.1038/...
Neurons carry ~5,000–10,000 molecules of PI(4,5)P₂ per μm² of membrane. What happens to human brain development when that number changes? I address this using a disease-in-a-dish model of Lowe Syndrome:
www.embopress.org/doi/10.1038/...
Reposted by Yojet
New blog post, where I highlight a few interesting papers on astrocyte ultrastructure and function, with papers from the labs of Moritz Helmstädter @mhlab.bsky.social, Kristen Harris, Andrea Volterra, Karin Pernet-Gallay, and Hailan Hu: gcamp6f.com/2025/11/14/i...
Interesting papers on astrocyte ultrastructure and function
Astrocytes are brain cells that are often completely overlooked and dismissed or, in the opposite extreme, presented as mysterious devices that somehow solve all problems of computational neuroscie…
gcamp6f.com
November 14, 2025 at 12:27 PM
New blog post, where I highlight a few interesting papers on astrocyte ultrastructure and function, with papers from the labs of Moritz Helmstädter @mhlab.bsky.social, Kristen Harris, Andrea Volterra, Karin Pernet-Gallay, and Hailan Hu: gcamp6f.com/2025/11/14/i...
Reposted by Yojet
Enhanced #Notch dependent gliogenesis and delayed physiological maturation underlie neurodevelopmental defects in #LoweSyndrome
By Y. Sharma, P. Raghu et al @ncbsbangalore.bsky.social
🗞️ doi.org/10.1038/s443...
By Y. Sharma, P. Raghu et al @ncbsbangalore.bsky.social
🗞️ doi.org/10.1038/s443...
November 13, 2025 at 6:49 AM
Enhanced #Notch dependent gliogenesis and delayed physiological maturation underlie neurodevelopmental defects in #LoweSyndrome
By Y. Sharma, P. Raghu et al @ncbsbangalore.bsky.social
🗞️ doi.org/10.1038/s443...
By Y. Sharma, P. Raghu et al @ncbsbangalore.bsky.social
🗞️ doi.org/10.1038/s443...
Very excited to share that my PhD work is out!
Neurons carry ~5,000–10,000 molecules of PI(4,5)P₂ per μm² of membrane. What happens to human brain development when that number changes? I address this using a disease-in-a-dish model of Lowe Syndrome:
www.embopress.org/doi/10.1038/...
Neurons carry ~5,000–10,000 molecules of PI(4,5)P₂ per μm² of membrane. What happens to human brain development when that number changes? I address this using a disease-in-a-dish model of Lowe Syndrome:
www.embopress.org/doi/10.1038/...
November 11, 2025 at 6:53 PM
Very excited to share that my PhD work is out!
Neurons carry ~5,000–10,000 molecules of PI(4,5)P₂ per μm² of membrane. What happens to human brain development when that number changes? I address this using a disease-in-a-dish model of Lowe Syndrome:
www.embopress.org/doi/10.1038/...
Neurons carry ~5,000–10,000 molecules of PI(4,5)P₂ per μm² of membrane. What happens to human brain development when that number changes? I address this using a disease-in-a-dish model of Lowe Syndrome:
www.embopress.org/doi/10.1038/...
Do human iPSC derived microglia also possess similar electrophysiological properties?
www.cell.com/trends/neuro...
www.cell.com/trends/neuro...
Ion Channels and Receptors as Determinants of Microglial Function
Microglia provide immune surveillance of the CNS. They display diverse behaviors,
including nondirectional and directed motility of their processes, phagocytosis of
targets such as dying neurons or su...
www.cell.com
October 25, 2025 at 5:42 PM
Do human iPSC derived microglia also possess similar electrophysiological properties?
www.cell.com/trends/neuro...
www.cell.com/trends/neuro...
Reposted by Yojet
Latest work out today in @currentbiology.bsky.social
We find the fly development gene bicoid is much older than previously thought (~20 million yrs older!) 🪰🧬
To pinpoint its origins we tackled the Diptera phylogeny, providing some resolution (many open questions remain).
🔗 tinyurl.com/2vyuevpy
We find the fly development gene bicoid is much older than previously thought (~20 million yrs older!) 🪰🧬
To pinpoint its origins we tackled the Diptera phylogeny, providing some resolution (many open questions remain).
🔗 tinyurl.com/2vyuevpy
Revised evolutionary relationships within Brachycera and the early origin of bicoid in flies
Mulhair et al. uncover a functional bicoid in non-cyclorrhaphan flies, pushing the
gene's origin back by ∼20 million years. Reassessing the Diptera phylogeny using the
largest dataset to date permits ...
www.cell.com
October 17, 2025 at 3:14 PM
Latest work out today in @currentbiology.bsky.social
We find the fly development gene bicoid is much older than previously thought (~20 million yrs older!) 🪰🧬
To pinpoint its origins we tackled the Diptera phylogeny, providing some resolution (many open questions remain).
🔗 tinyurl.com/2vyuevpy
We find the fly development gene bicoid is much older than previously thought (~20 million yrs older!) 🪰🧬
To pinpoint its origins we tackled the Diptera phylogeny, providing some resolution (many open questions remain).
🔗 tinyurl.com/2vyuevpy
Reposted by Yojet
Research Briefing: Promoter editing generates stable setpoints of gene expression www.nature.com/articles/s41...
rdcu.be/eK2FW
rdcu.be/eK2FW
Promoter editing generates stable setpoints of gene expression - Nature Biotechnology
Precise control of transgene expression remains a challenge in engineering primary cells for diverse applications. We developed DIAL, a promoter editing framework that transmits transient inputs into ...
www.nature.com
October 15, 2025 at 12:57 PM
Research Briefing: Promoter editing generates stable setpoints of gene expression www.nature.com/articles/s41...
rdcu.be/eK2FW
rdcu.be/eK2FW
Reposted by Yojet
Beautiful live-cell imaging of lysosome tubulation along microtubules in this work just out from @lysoluis.bsky.social et al:
rupress.org/jcb/article/...
rupress.org/jcb/article/...
September 26, 2025 at 6:14 AM
Beautiful live-cell imaging of lysosome tubulation along microtubules in this work just out from @lysoluis.bsky.social et al:
rupress.org/jcb/article/...
rupress.org/jcb/article/...
Reposted by Yojet
Morphological heterogeneity of human astrocytes in cerebral organoids https://www.biorxiv.org/content/10.1101/2025.09.18.675430v1
September 19, 2025 at 7:16 AM
Morphological heterogeneity of human astrocytes in cerebral organoids https://www.biorxiv.org/content/10.1101/2025.09.18.675430v1
Reposted by Yojet
A phenotypic brain organoid atlas for neurodevelopmental disorders https://www.biorxiv.org/content/10.1101/2025.09.12.675864v1
September 13, 2025 at 8:16 AM
A phenotypic brain organoid atlas for neurodevelopmental disorders https://www.biorxiv.org/content/10.1101/2025.09.12.675864v1
Reposted by Yojet
For some three billion years, unicellular organisms ruled Earth. Then, around one billion years ago, a new chapter of life began
go.nature.com/3JyRV4S
go.nature.com/3JyRV4S
How did life get multicellular? Five simple organisms could have the answer
Single-celled species that often stick together in colonies have researchers rethinking the origin of animals.
go.nature.com
August 27, 2025 at 10:19 AM
For some three billion years, unicellular organisms ruled Earth. Then, around one billion years ago, a new chapter of life began
go.nature.com/3JyRV4S
go.nature.com/3JyRV4S
Reposted by Yojet
1
To predict the behaviour of a primate, would you rather base your guess on a closely related species or one with a similar brain shape? We looked at brains & behaviours of 70 species, you’ll be surprised!
🧵Thread on our new preprint with @r3rt0.bsky.social , doi.org/10.1101/2025...
To predict the behaviour of a primate, would you rather base your guess on a closely related species or one with a similar brain shape? We looked at brains & behaviours of 70 species, you’ll be surprised!
🧵Thread on our new preprint with @r3rt0.bsky.social , doi.org/10.1101/2025...
July 27, 2025 at 5:26 PM
1
To predict the behaviour of a primate, would you rather base your guess on a closely related species or one with a similar brain shape? We looked at brains & behaviours of 70 species, you’ll be surprised!
🧵Thread on our new preprint with @r3rt0.bsky.social , doi.org/10.1101/2025...
To predict the behaviour of a primate, would you rather base your guess on a closely related species or one with a similar brain shape? We looked at brains & behaviours of 70 species, you’ll be surprised!
🧵Thread on our new preprint with @r3rt0.bsky.social , doi.org/10.1101/2025...
Reposted by Yojet
Excited that my co-first author manuscript is now published! Special shoutout to Xuelong Mi in Guoqiang Yu's group, who did all of the development of this fantastic tool, which I’ve applied to segment neuromodulatory and astroglial calcium signals. www.cell.com/cell/fulltex...
Fast, accurate, and versatile data analysis platform for the quantification of molecular spatiotemporal signals
AQuA2 is a powerful data analysis platform that decomposes time-lapse imaging datasets
into elementary signaling events, allowing for accurate quantification of spatiotemporal
molecular activity patte...
www.cell.com
April 9, 2025 at 2:08 PM
Excited that my co-first author manuscript is now published! Special shoutout to Xuelong Mi in Guoqiang Yu's group, who did all of the development of this fantastic tool, which I’ve applied to segment neuromodulatory and astroglial calcium signals. www.cell.com/cell/fulltex...
Reposted by Yojet
☕️Ma et al. show that heterogeneous nuclear ribonucleoprotein U promotes the primed state in human #pluripotent stem cells by interacting with #NuclearMatrix protein, Matrin-3, and regulating primed-specific genes.
👉https://rdcu.be/ebq0W
bit.ly/4iiVHLV
👉https://rdcu.be/ebq0W
bit.ly/4iiVHLV
The nuclear matrix stabilizes primed-specific genes in human pluripotent stem cells - Nature Cell Biology
Ma et al. show that heterogeneous nuclear ribonucleoprotein U promotes the primed state in human pluripotent stem cells by interacting with nuclear matrix protein, Matrin-3, and regulating primed-spec...
bit.ly
February 26, 2025 at 9:12 PM
☕️Ma et al. show that heterogeneous nuclear ribonucleoprotein U promotes the primed state in human #pluripotent stem cells by interacting with #NuclearMatrix protein, Matrin-3, and regulating primed-specific genes.
👉https://rdcu.be/ebq0W
bit.ly/4iiVHLV
👉https://rdcu.be/ebq0W
bit.ly/4iiVHLV
Reposted by Yojet
PTEN mutations impair CSF dynamics and cortical networks by dysregulating periventricular neural progenitors 🧪🧠
www.nature.com/articles/s41...
www.nature.com/articles/s41...
PTEN mutations impair CSF dynamics and cortical networks by dysregulating periventricular neural progenitors - Nature Neuroscience
The authors show PTEN mutations, which can cause both congenital hydrocephalus and autism spectrum disorder, disrupt CSF homeostasis and brain connectivity in mice. Reducing mTORC1 activation ameliora...
www.nature.com
February 26, 2025 at 3:46 PM
PTEN mutations impair CSF dynamics and cortical networks by dysregulating periventricular neural progenitors 🧪🧠
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Yojet
I started my lab more then 4 years ago, and we have endured some very challenging times ever since.…I am very excited to share the first paper from my lab, published now in @naturemethods.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain - Nature Methods
A first-in-class fluorescence lifetime-based FRET sensor for phosphatase and tensin homolog (PTEN) enables the dynamic monitoring of PTEN activity in cultured cells and in vivo with high spatiotempora...
www.nature.com
February 20, 2025 at 1:10 PM
I started my lab more then 4 years ago, and we have endured some very challenging times ever since.…I am very excited to share the first paper from my lab, published now in @naturemethods.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Yojet
Voltage-gated ion channel diversity underlies neuronal excitability and nervous system evolution https://www.biorxiv.org/content/10.1101/2025.02.15.638427v1
February 16, 2025 at 10:16 AM
Voltage-gated ion channel diversity underlies neuronal excitability and nervous system evolution https://www.biorxiv.org/content/10.1101/2025.02.15.638427v1
Reposted by Yojet
I am excited to announce the release of our BioRxiv preprint: “Network-aware self-supervised learning enables high-content phenotypic screening for genetic modifiers of neuronal activity dynamics”. 1/
Network-aware self-supervised learning enables high-content phenotypic screening for genetic modifiers of neuronal activity dynamics
High-throughput phenotypic screening has historically relied on manually selected features, limiting our ability to capture complex cellular processes, particularly neuronal activity dynamics. While r...
www.biorxiv.org
February 6, 2025 at 5:15 PM
I am excited to announce the release of our BioRxiv preprint: “Network-aware self-supervised learning enables high-content phenotypic screening for genetic modifiers of neuronal activity dynamics”. 1/
Reposted by Yojet
New preprint! A really fun project spearheaded by amazing graduate student @christinaramelow.bsky.social and a collaboration with Srikant Rangaraju's group.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Simultaneous profiling of native-state proteomes and transcriptomes of neural cell types using proximity labeling
Deep molecular phenotyping of cells at transcriptomic and proteomic levels is an essential first step to understanding cellular contributions to development, aging, injury, and disease. Since proteome...
www.biorxiv.org
February 3, 2025 at 9:12 PM
New preprint! A really fun project spearheaded by amazing graduate student @christinaramelow.bsky.social and a collaboration with Srikant Rangaraju's group.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Yojet
#GliaPapers #GliaSpecialIssue
SPECIAL COVER
SPECIAL ISSUE: Glial omics: unraveling mechanisms in health and disease
onlinelibrary.wiley.com/toc/10981136...
Editorial Unboxing “Omics” in Glial Biology to Understand Neurological Disease here - onlinelibrary.wiley.com/doi/10.1002/...
SPECIAL COVER
SPECIAL ISSUE: Glial omics: unraveling mechanisms in health and disease
onlinelibrary.wiley.com/toc/10981136...
Editorial Unboxing “Omics” in Glial Biology to Understand Neurological Disease here - onlinelibrary.wiley.com/doi/10.1002/...
Special Issue: Glial omics: Unraveling mechanisms in health and disease: Glia: Vol 73, No 3
Glia is the top neurobiology journal for research into glial cells (also called gliocytes or neuroglia). Articles cover all aspects of glial cell biology in health and disease.
onlinelibrary.wiley.com
January 31, 2025 at 5:35 PM
#GliaPapers #GliaSpecialIssue
SPECIAL COVER
SPECIAL ISSUE: Glial omics: unraveling mechanisms in health and disease
onlinelibrary.wiley.com/toc/10981136...
Editorial Unboxing “Omics” in Glial Biology to Understand Neurological Disease here - onlinelibrary.wiley.com/doi/10.1002/...
SPECIAL COVER
SPECIAL ISSUE: Glial omics: unraveling mechanisms in health and disease
onlinelibrary.wiley.com/toc/10981136...
Editorial Unboxing “Omics” in Glial Biology to Understand Neurological Disease here - onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Yojet
As promised, here is our NEW preprint on CELL CYCLE ASSOCIATED PIP2 NUCLEAR METABOLISM by our SUPER postdoc Toni Gañez, who knows all the secrets of FUCCI reporters 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
January 3, 2025 at 8:41 AM
As promised, here is our NEW preprint on CELL CYCLE ASSOCIATED PIP2 NUCLEAR METABOLISM by our SUPER postdoc Toni Gañez, who knows all the secrets of FUCCI reporters 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
One of those classical papers that anyone interested in iPSC-NSC biology should read.
Vertebrate Embryonic Cells Will Become Nerve Cells Unless Told Otherwise: Cell www.cell.com/cell/fulltex...
Vertebrate Embryonic Cells Will Become Nerve Cells Unless Told Otherwise: Cell www.cell.com/cell/fulltex...
Vertebrate Embryonic Cells Will Become Nerve Cells Unless Told Otherwise
The past few years have witnessed a significant change in the understanding of how
the vertebrate nervous system forms during embryogenesis. More than seventy years
since Spemann and Mangold first dem...
www.cell.com
January 21, 2025 at 6:50 PM
One of those classical papers that anyone interested in iPSC-NSC biology should read.
Vertebrate Embryonic Cells Will Become Nerve Cells Unless Told Otherwise: Cell www.cell.com/cell/fulltex...
Vertebrate Embryonic Cells Will Become Nerve Cells Unless Told Otherwise: Cell www.cell.com/cell/fulltex...
Reposted by Yojet
Hi #neuroscience community! This is one of my favorite posts ever. In close collaboration with @liddelowsa.bsky.social and Ye Zhang, we've built a new tool that we are eager to share! www.webseq.net
WEBseq
share freely
www.webseq.net
January 15, 2025 at 4:21 PM
Hi #neuroscience community! This is one of my favorite posts ever. In close collaboration with @liddelowsa.bsky.social and Ye Zhang, we've built a new tool that we are eager to share! www.webseq.net
Reposted by Yojet
Our second preprint, which was spearheaded by @thomasdwkim.bsky.social, uses single-cell multiomic analysis to identify gene regulatory networks controlling neurogenesis and cell fate specification in mouse hypothalamus and prethalamus./1
biorxiv.org/cgi/content/...
biorxiv.org/cgi/content/...
January 12, 2025 at 3:08 PM
Our second preprint, which was spearheaded by @thomasdwkim.bsky.social, uses single-cell multiomic analysis to identify gene regulatory networks controlling neurogenesis and cell fate specification in mouse hypothalamus and prethalamus./1
biorxiv.org/cgi/content/...
biorxiv.org/cgi/content/...
Reposted by Yojet
First 🦋 post! Very excited to share our lab's latest work exploring the shared and divergent aspects of human astrocyte development and glioblastoma. This effort spans many fields from developmental #glial biology to #stem cells and #tumor biology. rdcu.be/d5ADz A 🧵
Mapping the developmental trajectory of human astrocytes reveals divergence in glioblastoma
Nature Cell Biology - Sojka et al. analyse the transcriptomic and epigenomic landscape of human astrocyte maturation and identify an epigenetically regulated intermediate state associated with...
rdcu.be
January 8, 2025 at 5:13 PM
First 🦋 post! Very excited to share our lab's latest work exploring the shared and divergent aspects of human astrocyte development and glioblastoma. This effort spans many fields from developmental #glial biology to #stem cells and #tumor biology. rdcu.be/d5ADz A 🧵
Reposted by Yojet
How to do differential expression with scRNAseq data? State of the art is "pseudo-bulk" analysis with RNA-seq methods like edgeR or DESeq2, where "cell type" is encoded as discrete categories. Biologically, discrete categories are not always the most appropriate concept.(1/3)
doi.org/10.1038/s415...
doi.org/10.1038/s415...
Analysis of multi-condition single-cell data with latent embedding multivariate regression - Nature Genetics
Latent embedding multivariate regression models multi-condition single-cell RNA-seq using a continuous latent space, enabling data integration, per-cell gene expression prediction and clustering-free ...
doi.org
January 3, 2025 at 7:18 PM
How to do differential expression with scRNAseq data? State of the art is "pseudo-bulk" analysis with RNA-seq methods like edgeR or DESeq2, where "cell type" is encoded as discrete categories. Biologically, discrete categories are not always the most appropriate concept.(1/3)
doi.org/10.1038/s415...
doi.org/10.1038/s415...