Furthermore, inhibition of actin polymerization (Latrunculin A) and actin branching (CK-666) disrupted rotation and cryptic lamellipodia formation, highlighting the importance of actin organization.
December 6, 2025 at 4:43 AM
Furthermore, inhibition of actin polymerization (Latrunculin A) and actin branching (CK-666) disrupted rotation and cryptic lamellipodia formation, highlighting the importance of actin organization.
Issue 22 is complete
Explore our ToC: journals.biologists.com/jcs/issue/13...
Our cover image shows Cyri-b-knockout cells with actin-driven lamellipodia in blue and microtubules in orange. See article by Whitelaw, et al.
journals.biologists.com/jcs/article/...
Explore our ToC: journals.biologists.com/jcs/issue/13...
Our cover image shows Cyri-b-knockout cells with actin-driven lamellipodia in blue and microtubules in orange. See article by Whitelaw, et al.
journals.biologists.com/jcs/article/...
December 4, 2025 at 11:44 AM
Issue 22 is complete
Explore our ToC: journals.biologists.com/jcs/issue/13...
Our cover image shows Cyri-b-knockout cells with actin-driven lamellipodia in blue and microtubules in orange. See article by Whitelaw, et al.
journals.biologists.com/jcs/article/...
Explore our ToC: journals.biologists.com/jcs/issue/13...
Our cover image shows Cyri-b-knockout cells with actin-driven lamellipodia in blue and microtubules in orange. See article by Whitelaw, et al.
journals.biologists.com/jcs/article/...
Lamellipodia-saurus wishes everyone a happy Halloween
October 31, 2025 at 5:48 PM
Lamellipodia-saurus wishes everyone a happy Halloween
Force loading on molecular clutches governs the stability of cell lamellipodia https://www.biorxiv.org/content/10.1101/2025.10.10.679903v1
October 10, 2025 at 9:48 PM
Force loading on molecular clutches governs the stability of cell lamellipodia https://www.biorxiv.org/content/10.1101/2025.10.10.679903v1
Great to see our conclusions align with the beautiful work from @marija-matejcic.bsky.social in the Trepat lab 3D force mapping, Ca²⁺-triggered myosin-IIA meshwork dissolution, and neighbour lamellipodia generating traction. Great synergy, highlighting the role of tension in extrusion regulation.
September 12, 2025 at 11:15 AM
Great to see our conclusions align with the beautiful work from @marija-matejcic.bsky.social in the Trepat lab 3D force mapping, Ca²⁺-triggered myosin-IIA meshwork dissolution, and neighbour lamellipodia generating traction. Great synergy, highlighting the role of tension in extrusion regulation.
Not only that, the N-ter alone actually promoted CDR formation over lamellipodia formation when overexpressed in wild type cells - and increased 3D invasiveness. 9/n
August 2, 2025 at 1:27 PM
Not only that, the N-ter alone actually promoted CDR formation over lamellipodia formation when overexpressed in wild type cells - and increased 3D invasiveness. 9/n
The N-ter wasn't localised to FAs but suppressed lamellipodia formation overall and restored corner bias (and reduced motility in 2D but restored directionality). The C-ter promoted lamellipodia all over, with a bit of corner bias, and localised to the nucleus. 8/n
August 2, 2025 at 1:25 PM
The N-ter wasn't localised to FAs but suppressed lamellipodia formation overall and restored corner bias (and reduced motility in 2D but restored directionality). The C-ter promoted lamellipodia all over, with a bit of corner bias, and localised to the nucleus. 8/n
Even weirder, the two halves of paxillin - the LD motif containing N-ter and the LIM domain containing C-ter - had opposite effects on both CDR and lamellipodia formation, and could rescue the -/- lateral corner bias phenotype if co-expressed. 7/n
August 2, 2025 at 1:21 PM
Even weirder, the two halves of paxillin - the LD motif containing N-ter and the LIM domain containing C-ter - had opposite effects on both CDR and lamellipodia formation, and could rescue the -/- lateral corner bias phenotype if co-expressed. 7/n
But this corner bias does not happen right away! There's a first round of membrane ruffling and F-actin assembly with no bias - lateral and dorsal. Circular dorsal ruffles (CDRs) form on top and lamellipodia from all side regions in the first 5-15 min, then fall back if they can't adhere. 3/n
August 2, 2025 at 1:15 PM
But this corner bias does not happen right away! There's a first round of membrane ruffling and F-actin assembly with no bias - lateral and dorsal. Circular dorsal ruffles (CDRs) form on top and lamellipodia from all side regions in the first 5-15 min, then fall back if they can't adhere. 3/n
When cells have spread fully, adding a motogen like PDGF stimulates F-actin protrusion formation, and lamellipodia also have a corner localisation bias (i.e. in rhe vicinity of FAs). In the absence of paxillin, and lots of small FAs all around, cells lose corner lamellipodia bias. 2/n
August 2, 2025 at 1:12 PM
When cells have spread fully, adding a motogen like PDGF stimulates F-actin protrusion formation, and lamellipodia also have a corner localisation bias (i.e. in rhe vicinity of FAs). In the absence of paxillin, and lots of small FAs all around, cells lose corner lamellipodia bias. 2/n
The latest on the 🌊 complex from my PhD lab. Super cool experiments to unravel mechanistically how the WAVE complex templates lamellipodia in migrating cells. Congrats Muziyue!
In new @jcb.org study from Muziyue Wu et al. @oweinerlab.bsky.social, in vivo biochemistry sheds new light on how the WAVE complex templates the characteristic shape of lamellipodial protrusions. rupress.org/jcb/article/...
#Cytoskeleton #Actin #Migration #Motility #Biophysics
#Cytoskeleton #Actin #Migration #Motility #Biophysics
July 23, 2025 at 9:20 PM
The latest on the 🌊 complex from my PhD lab. Super cool experiments to unravel mechanistically how the WAVE complex templates lamellipodia in migrating cells. Congrats Muziyue!
WAVE complex forms linear arrays at negative membrane curvature to instruct #lamellipodia formation, say Muziyue Wu, Orion Weiner @oweinerlab.bsky.social and colleagues: rupress.org/jcb/article/...
#Biophysics #Cytoskeleton #Migration #Motility #Actin
#Biophysics #Cytoskeleton #Migration #Motility #Actin
July 23, 2025 at 6:45 PM
WAVE complex forms linear arrays at negative membrane curvature to instruct #lamellipodia formation, say Muziyue Wu, Orion Weiner @oweinerlab.bsky.social and colleagues: rupress.org/jcb/article/...
#Biophysics #Cytoskeleton #Migration #Motility #Actin
#Biophysics #Cytoskeleton #Migration #Motility #Actin
WAVE complex forms linear arrays at negative membrane curvature to instruct lamellipodia formation url: rupress.org/jcb/article-...
WAVE complex forms linear arrays at negative membrane curvature to instruct lamellipodia formation
The WAVE complex is an actin nucleator that regulates sheet-like lamellipodial protrusions. M. Wu et al. find that the WAVE complex is a core and limiting
rupress.org
July 16, 2025 at 2:21 PM
WAVE complex forms linear arrays at negative membrane curvature to instruct lamellipodia formation url: rupress.org/jcb/article-...
On cell extrusion in the #intestine!
After 80 years of observations, we finally took a deeper look thanks to 2D #organoids. We report:
3D #forces, #extrusion still only in the villus even without curvature, #lamellipodia generate 3D force.. and more. Have a look!
www.biorxiv.org/content/10.1...
After 80 years of observations, we finally took a deeper look thanks to 2D #organoids. We report:
3D #forces, #extrusion still only in the villus even without curvature, #lamellipodia generate 3D force.. and more. Have a look!
www.biorxiv.org/content/10.1...
July 4, 2025 at 10:21 AM
On cell extrusion in the #intestine!
After 80 years of observations, we finally took a deeper look thanks to 2D #organoids. We report:
3D #forces, #extrusion still only in the villus even without curvature, #lamellipodia generate 3D force.. and more. Have a look!
www.biorxiv.org/content/10.1...
After 80 years of observations, we finally took a deeper look thanks to 2D #organoids. We report:
3D #forces, #extrusion still only in the villus even without curvature, #lamellipodia generate 3D force.. and more. Have a look!
www.biorxiv.org/content/10.1...
Homodimerized Arpins: Binding to Arp2/3 Complexes with Positive Synergy to Inhibit Lamellipodia-Dependent Tumor Metastasis https://www.biorxiv.org/content/10.1101/2025.06.24.661301v1
June 24, 2025 at 9:48 PM
Homodimerized Arpins: Binding to Arp2/3 Complexes with Positive Synergy to Inhibit Lamellipodia-Dependent Tumor Metastasis https://www.biorxiv.org/content/10.1101/2025.06.24.661301v1
Homodimerized Arpins: Binding to Arp2/3 Complexes with Positive Synergy to Inhibit Lamellipodia-Dependent Tumor Metastasis https://www.biorxiv.org/content/10.1101/2025.06.24.661301v1
June 24, 2025 at 9:48 PM
Homodimerized Arpins: Binding to Arp2/3 Complexes with Positive Synergy to Inhibit Lamellipodia-Dependent Tumor Metastasis https://www.biorxiv.org/content/10.1101/2025.06.24.661301v1
Another #snoutscope imaging session at the UCSF Center for Advanced Light Microscopy! This time, with neutrophils! Courtesy of Patrick Zager in the Weiner lab.
Neutrophil plasma membranes are labeled to show 3D filopodia and lamellipodia dynamics at 1 volume/second.
Neutrophil plasma membranes are labeled to show 3D filopodia and lamellipodia dynamics at 1 volume/second.
June 24, 2025 at 1:31 AM
Another #snoutscope imaging session at the UCSF Center for Advanced Light Microscopy! This time, with neutrophils! Courtesy of Patrick Zager in the Weiner lab.
Neutrophil plasma membranes are labeled to show 3D filopodia and lamellipodia dynamics at 1 volume/second.
Neutrophil plasma membranes are labeled to show 3D filopodia and lamellipodia dynamics at 1 volume/second.
New study using a mechanical model to investigate how stress fiber and lamellipodia dynamics regulate collective cell migration reveals that strengthened stress fibers enhance cell rearrangements and stress alignment to promote coordinated movement. www.cell.com/biophysj/ful...
June 19, 2025 at 2:03 PM
New study using a mechanical model to investigate how stress fiber and lamellipodia dynamics regulate collective cell migration reveals that strengthened stress fibers enhance cell rearrangements and stress alignment to promote coordinated movement. www.cell.com/biophysj/ful...
Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics pubmed.ncbi.nlm.nih.gov/40475257/ #cryoem
June 8, 2025 at 2:53 AM
Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics pubmed.ncbi.nlm.nih.gov/40475257/ #cryoem
ICYMI: New online: UFMylation of ARPC4 facilitates lamellipodia formation and promotes cancer metastasis
UFMylation of ARPC4 facilitates lamellipodia formation and promotes cancer metastasis
Nature Structural & Molecular Biology, Published online: 26 May 2025; doi:10.1038/s41594-025-01577-7Cell migration is a prerequisite for cancer cell invasion and metastasis. The highlighted study shows that UFMylation of ARPC4 facilitates ARP2/3 complex-mediated lamellipodia formation, thereby promoting cancer cell migration, invasion and metastasis.
go.nature.com
May 27, 2025 at 11:49 AM
ICYMI: New online: UFMylation of ARPC4 facilitates lamellipodia formation and promotes cancer metastasis
New online: UFMylation of ARPC4 facilitates lamellipodia formation and promotes cancer metastasis
UFMylation of ARPC4 facilitates lamellipodia formation and promotes cancer metastasis
Nature Structural & Molecular Biology, Published online: 26 May 2025; doi:10.1038/s41594-025-01577-7Cell migration is a prerequisite for cancer cell invasion and metastasis. The highlighted study shows that UFMylation of ARPC4 facilitates ARP2/3 complex-mediated lamellipodia formation, thereby promoting cancer cell migration, invasion and metastasis.
go.nature.com
May 26, 2025 at 11:48 AM
New online: UFMylation of ARPC4 facilitates lamellipodia formation and promotes cancer metastasis
Ankrd1 Promotes Lamellipodia Formation and Cell Motility via Interaction with Talin-1 in Clear Cell Renal Cell Carcinoma
www.mdpi.com/1422-0067/26...
www.mdpi.com/1422-0067/26...
www.mdpi.com
May 20, 2025 at 5:23 PM
Ankrd1 Promotes Lamellipodia Formation and Cell Motility via Interaction with Talin-1 in Clear Cell Renal Cell Carcinoma
www.mdpi.com/1422-0067/26...
www.mdpi.com/1422-0067/26...
This method reveals actin patches, cables, rings (yeast), and cortex, filopodia, lamellipodia, stress fibers (cell lines). Notably, it captures nuclear actin filaments with high specificity highlighting IntAct-U-ExM’s power to resolve nanoscale actin architecture across species.
May 16, 2025 at 8:00 AM
This method reveals actin patches, cables, rings (yeast), and cortex, filopodia, lamellipodia, stress fibers (cell lines). Notably, it captures nuclear actin filaments with high specificity highlighting IntAct-U-ExM’s power to resolve nanoscale actin architecture across species.
Mono-Ubiquitylation-Dependent Rap2 Activation Regulates Lamellipodia Dynamics During Cell Migration https://www.biorxiv.org/content/10.1101/2025.04.25.650668v1
April 29, 2025 at 1:34 PM
Mono-Ubiquitylation-Dependent Rap2 Activation Regulates Lamellipodia Dynamics During Cell Migration https://www.biorxiv.org/content/10.1101/2025.04.25.650668v1
Mono-Ubiquitylation-Dependent Rap2 Activation Regulates Lamellipodia Dynamics During Cell Migration https://www.biorxiv.org/content/10.1101/2025.04.25.650668v1
April 29, 2025 at 1:34 PM
Mono-Ubiquitylation-Dependent Rap2 Activation Regulates Lamellipodia Dynamics During Cell Migration https://www.biorxiv.org/content/10.1101/2025.04.25.650668v1