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mlacyphd.bsky.social
Mike Lacy
@mlacyphd.bsky.social
1.1K followers 1.1K following 140 posts
#OpenScience supporter, I work @Addgene.bsky.social curating DNA and web content. Previously worked at ASCB, publishing, Yale Biophysics PhD. He/him
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mlacyphd.bsky.social
Mike Lacy @mlacyphd.bsky.social · Nov 12
👋 Intro for new folks:
I'm a scientific curator at @addgene.bsky.social. You might see my blog posts or get emails from me about depositing plasmids. Our team mainly works behind the scenes cleaning up data, making/updating webpages, and more. I'm always on the lookout for cool new science to share!
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Reposted by Mike Lacy
addgene.bsky.social
Addgene @addgene.bsky.social · 7h
Chonnettia Jones, Addgene’s President & Executive Director, is one of Endpoints News’ 2025 Top Women in Biopharma R&D! She is driving Addgene’s growth to enable more discovery and innovation, powered by the sharing of scientific resources.
🔗
endpoints.news
Meet 20 women reshaping biopharma, from early discovery to patient advocacy
twp.ai
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Reposted by Mike Lacy
addgene.bsky.social
Addgene @addgene.bsky.social · 2d
New CRISPR libraries from the @johndoench.bsky.social and Root labs — Jacquere (human) and Julianna (mouse).
A perfect pairing for your next screen. 🍷🧀
https://twp.ai/9PX2kT
https://twp.ai/4irnYP
new at addgene
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Reposted by Mike Lacy
needhibhalla.bsky.social
Needhi Bhalla 💅🏽 @needhibhalla.bsky.social · 4d
There’s some things I disagree with here but this is absolutely on point: “Science moves faster when we write for each other rather than for gatekeepers...The transformation of scientific communication requires scientists willing to experiment and builders ready to support them.”
pracheeac.bsky.social
Prachee Avasthi @pracheeac.bsky.social · 4d
New Blog Post:
Scholarly Communication Is a Research Problem. This Means You.

pracheeac.substack.com/p/scholarly-...
Scholarly Communication Is a Research Problem. This Means You.
Scholarly Communication Is a Research Problem.
pracheeac.substack.com
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Reposted by Mike Lacy
scattercushion.bsky.social
Amanda Heidt @scattercushion.bsky.social · 6d
🧪New from me for @nature.com: Nearly every model organism used in research has it's own database, called a MOD. These resources underpin decades of discovery, but they're collectively struggling amid funding cuts.

www.nature.com/articles/d41...
Model organism databases face budget cuts and closures
Beyond the crucial data they contain, these digital archives have provided an important space for academic communities to exchange ideas and resources.
www.nature.com
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Reposted by Mike Lacy
allostericsite.bsky.social
Eric J. Perkins, PhD (he/him) @allostericsite.bsky.social · 6d
These plasmids from @promegacorporation.bsky.social are exceptional new offerings from Addgene for a few reasons:
- Since there is no MTA, they are available to industry customers (joining an ever-growing catalog subset: www.addgene.org/industry/)
- They are available in a DNA format!
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Reposted by Mike Lacy
addgene.bsky.social
Addgene @addgene.bsky.social · 7d
New AAV tools from @e11bio.bsky.social! PRISM uses protein-tag barcodes for high-throughput reconstruction of neurons and other cell-labeling applications. https://twp.ai/4iruEB
A 3D rendering of a network of neurons from a rectangular volume. Each neuron is filled with a slightly different color, making each cell body and its projections distinct from its neighbors.
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joachimgoedhart.bsky.social
Joachim Goedhart @joachimgoedhart.bsky.social · 8d
Identification of optimal fluorophores for use in the Drosophila embryo by Timothy E Saunders and team: www.biorxiv.org/content/10.1...
Figure 1: Fluorophore intensity in the Drosophila blastoderm (n.c. 14). Comparison of (A) green and (B) red fluorescence intensity using the same intensity scaling in n.c. 14. The fluorescence signal did not saturate. Shown are single imaging planes. Normalised histograms of fluorescence intensity for green (C) and red (D) fluorophores averaged across at least n=3 embryos per line. Scale bars = 20 μm
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johanduchene.bsky.social
Johan Duchêne @johanduchene.bsky.social · 9d
⚠️ Half of commercial antibodies miss their target!
So how do you pick the right one for your experiment?

Here’s the solution 👉
Antibody characterization data (all tested in KO cells) are now available at:
🔗 onlygoodantibodies.co.uk

Tested by @ycharos.bsky.social & @oga-community.bsky.social
OGA - Only Good Antibodies Community
onlygoodantibodies.co.uk
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Reposted by Mike Lacy
needhibhalla.bsky.social
Needhi Bhalla 💅🏽 @needhibhalla.bsky.social · 13d
"the benefits of NIH research in general are wide and diffuse. A very large number of advances—roughly one in two during the 21st century—are linked to NIH grants that are cut in our counterfactual scenario."
drugmonkey.bsky.social
Drug Monkey @drugmonkey.bsky.social · 13d
This may be the most important paper ever published about NIH funded research.

www.science.org/doi/10.1126/...
What if NIH had been 40% smaller?
Replaying history with less NIH funding shows widespread impacts on drug-linked research
www.science.org
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jeremymberg.bsky.social
Jeremy Berg @jeremymberg.bsky.social · 12d
This paper does a great job with a "It's a Wonderful Life" scenario about NIH, supposing the consequences of the bottom 40% of the funding NIH grants never existed.

www.science.org/doi/10.1126/...

tl:dr The world would lose a lot, but directly and indirectly
What if NIH had been 40% smaller?
Replaying history with less NIH funding shows widespread impacts on drug-linked research
www.science.org
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Reposted by Mike Lacy
ipiproteins.bsky.social
Institute for Protein Innovation @ipiproteins.bsky.social · 15d
Frequent user of epitope tags? Try ours!

IPI epitope tag antibodies & plasmids are now available through our partner @addgene.bsky.social!

buff.ly/YEuZDIp
Epitope tag antibody collection
Epitope tags are short amino acid sequences fused to a protein’s N- or C-terminus. When paired with an antibody that specifically recognizes this sequence, epit
buff.ly
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Reposted by Mike Lacy
prelights.bsky.social
preLights @prelights.bsky.social · 15d
preLights is a #preprint highlighting service—supported by @biologists.bsky.social—encouraging #ECRs to highlight and discuss preprints that spark interest or debate within the biological #community. #SciComm

Interested in joining? Our 'call for preLighters' is open for another week! ⬇️👀
prelights.bsky.social
preLights @prelights.bsky.social · Sep 1
🚨 New call for preLighters!🚨

Help us highlight the most exciting biological #preprints. Share your insights, build your profile, and be part of a supportive community! 🌍

Interested? Apply today & help shape the future of science publishing! 📢

More info: prelights.biologists.com/news/join-th...
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Reposted by Mike Lacy
addgene.bsky.social
Addgene @addgene.bsky.social · 16d
The expanded Promega Collection is here! 250+ new plasmids for cancer research, protein interactions, targeted degradation & more.
✔ Industry + academic access
✔ No MTA required
✔ Aliquots or stabs to fit your workflow
Check it out: https://twp.ai/4iqKEV
Explore the Expanded Promega Collection, with Addgene and Promega logos and the Blugene mascot holding a light bulb
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Reposted by Mike Lacy
labliston.bsky.social
Adrian Liston @labliston.bsky.social · 16d
My only regret is that we didn't go with the title "One buffer to rule them all, and in the darkness bind them", although "Squeaky clean staining" was a pretty good options too!
labliston.bsky.social
Adrian Liston @labliston.bsky.social · 19d
For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?

You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
currentprotocols.onlinelibrary.wiley.com
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Reposted by Mike Lacy
radinbio.bsky.social
Ivan Radin @radinbio.bsky.social · 23d
Do you know what happens when you touch a carnivorous sundew plant?
If the touch is strong and large enough, a cytosolic calcium wave will spread from the site of touch throughout the whole plant, but if you only touch one tentacle (see post below), the calcium wave will be local and less intense.
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Reposted by Mike Lacy
addgene.bsky.social
Addgene @addgene.bsky.social · 19d
New at Addgene!
Three new yeast toolkits are now available for the community. Details in thread ⬇️
https://twp.ai/4iqKvQ
New at Addgene!
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Reposted by Mike Lacy
rashmi-priya.bsky.social
Rashmi Priya @rashmi-priya.bsky.social · 19d
Development does not happen in isolation! Material properties, geometry, stiffness, fluid flows...can all steer how development unfolds. If you’re exploring these kinds of questions, we’d love to see your work @dev-journal.bsky.social
More info below ⬇️
dev-journal.bsky.social
Development @dev-journal.bsky.social · Sep 1
📢Call for papers. Submit your latest research to our upcoming special issue – The Extracellular Environment in Development, Regeneration and Stem Cells

Guest Editors: Alex Hughes and Rashmi Priya

📅 Deadline: 1 March 2026

journals.biologists.com/dev/pages/ex...

#DevBio
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Reposted by Mike Lacy
christlet.bsky.social
Christophe 🔬 L @christlet.bsky.social · 19d
It's #FluorescenceFriday! Another movie from our preprint, showing exocytosis of VAMP2-pHluorin (live-cell TIRF, blue) correlated with 2-color STORM of spectrins (green) and clathrin-coated pits (magenta).
www.biorxiv.org/content/10.1...
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mlacyphd.bsky.social
Mike Lacy @mlacyphd.bsky.social · 19d
👀"robust treadmilling requires an optimal phase separation strength to maintain high local concentration while preserving condensate fluidity. A weakened phase separation fails to stabilize the bundles, whereas overly cohesive condensates impede filament dynamics." #actin #phaseseparation
myosinactncrazy.bsky.social @myosinactncrazy.bsky.social · 19d
Phase separation strength controls actin filament treadmilling

www.biorxiv.org/content/10.1...
Phase separation strength controls actin filament treadmilling
Cellular motility relies on the dynamic turnover of actin filaments, which treadmill through continuous polymerization at the barbed end and disassembly at the pointed end. Yet, the underlying physica...
www.biorxiv.org
Reposted by Mike Lacy
gek0s.bsky.social
Georg Kosche @gek0s.bsky.social · 23d
Gene editing of single, targeted neurons in vivo is now feasible. We are proud to present our preprint for highly efficient single-cell electroporation using RNA. With @alex-fratzl.bsky.social, @munzlab.bsky.social, Botond Roska @iobswiss.bsky.social
#neuroskyence
www.biorxiv.org/content/10.1...
In vivo single-cell gene editing using RNA electroporation reveals sequential adaptation of cortical neurons to excitatory-inhibitory imbalance
The balance between excitatory and inhibitory neurotransmission is fundamental for normal brain function, yet the adaptation of individual neurons to disrupted excitatory-inhibitory balance is not wel...
www.biorxiv.org
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Reposted by Mike Lacy
addgene.bsky.social
Addgene @addgene.bsky.social · 22d
Baby KJ's metabolic disorder was treated through an intense six-month effort to create a personalized CRISPR base editor — made possible by the preceding nine years of base editing research. Our latest blog post lays out the timeline of the advances that went into this year's major milestone.
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Reposted by Mike Lacy
ludowaltman.bsky.social
Ludo Waltman @ludowaltman.bsky.social · 25d
"Open access policymaking should ... focus less on freely accessible publications (which are no doubt important) and more on the processes and forms of governance that go into good publishing."
samuelmoore.org
Samuel Moore @samuelmoore.org · 27d
I have an op-ed out today in @resprofnews.bsky.social introducing my new book on the commercialisation of open access publishing.
Make academic publishing a commons, not a market - Research Professional News
Commercialisation has thwarted the promise of openness—it’s time for new priorities, says Samuel Moore
www.researchprofessionalnews.com
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alleninstitute.org
Allen Institute @alleninstitute.org · 23d
What is open science? 🤔

To celebrate #OpenScienceWeek, we're sharing why we give away our data, protocols, and resources to everyone.

Featuring our #openscience friends at @chanzuckerberg.bsky.social, NASA, @addgene.bsky.social, and NIH.
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addgene.bsky.social
Addgene @addgene.bsky.social · 23d
New tools available!
Plasmids deposited by the Mingshan Xue Lab expand the optogenetics toolkit with potent, blue-shifted channelrhodopsins.
https://twp.ai/4iqCaZ
new at addgene
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Reposted by Mike Lacy
docsirianni.bsky.social
Rachael Sirianni, PhD @docsirianni.bsky.social · 24d
I am not a political pawn. I am a living, breathing human being who has dedicated her life to a scientific problem that I believe matters for human health. The article below tells our story; I hope you will read it and share it www.nytimes.com/2025/09/14/m...
Trump Is Shutting Down the War On Cancer
www.nytimes.com
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